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A human airway smooth muscle cell line that retains physiological responsiveness
Abstract
We report the development of a nontransformed line of human airway smooth muscle cells retaining smooth muscle-specific contractile protein expression and physiological responsiveness to agonists implicated in inflammatory airway diseases. Specific responses to histamine, leukotrienes, bradykinin, platelet-activating factor, substance P, and thromboxane analogues are demonstrated as well as functional coupling to beta-adrenergic receptors. The cell line was characterized using indirect immunofluorescence, as well as electrophoretic separation and immunoblot analysis of smooth muscle-specific actin. Functional responses were assessed by measurements of cytosolic calcium and stimulation of adenosine 3',5'-cyclic monophosphate production. The cells retain their responsiveness over many population doublings and should be a useful model to examine specific receptor-effector mechanisms, as well as the effects of neurohumoral agents on the regulation of airway smooth muscle growth and differentiation.
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Metadata
- Authors
- Panettieri, R. A., Murray, R. K., DePalo, L. R., Yadvish, P. A., & Kotlikoff, M. I.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 1989-02-01
- Volume
- 256
- Issue
- 2
- Pages
- C329-C335
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W1875615308
- Cited by
- 325
- References
- 0
- FWCI
- 2.64
- Type
- journal article
- Language
- en
- Open access
- closed
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- Panettieri, R. A., Murray, R. K., DePalo, L. R., Yadvish, P. A., & Kotlikoff, M. I. (1989). A human airway smooth muscle cell line that retains physiological responsiveness. American Journal of Physiology-Cell Physiology. https://doi.org/10.1152/ajpcell.1989.256.2.c329
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