The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Reinforcement versus Fluidization in Cytoskeletal Mechanoresponsiveness
Open accessAbstract
Every adherent eukaryotic cell exerts appreciable traction forces upon its substrate. Moreover, every resident cell within the heart, great vessels, bladder, gut or lung routinely experiences large periodic stretches. As an acute response to such stretches the cytoskeleton can stiffen, increase traction forces and reinforce, as reported by some, or can soften and fluidize, as reported more recently by our laboratory, but in any given circumstance it remains unknown which response might prevail or why. Using a novel nanotechnology, we show here that in loading conditions expected in most physiological circumstances the localized reinforcement response fails to scale up to the level of homogeneous cell stretch; fluidization trumps reinforcement. Whereas the reinforcement response is known to be mediated by upstream mechanosensing and downstream signaling, results presented here show the fluidization response to be altogether novel: it is a direct physical effect of mechanical force acting upon a structural lattice that is soft and fragile. Cytoskeletal softness and fragility, we argue, is consistent with early evolutionary adaptations of the eukaryotic cell to material properties of a soft inert microenvironment.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Cell locomotion and focal adhesions are regulated by substrate flexibility
Pelham, R. J., & Wang, Y.-L. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.13661 · Cited by 3,027
Cells lying on a bed of microneedles: An approach to isolate mechanical force
Tan, J. L., Tien, J., Pirone, D. M., Gray, D. S., Bhadriraju, K., & Chen, C. S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0235407100 · Cited by 1,962
Silicone Rubber Substrata: A New Wrinkle in the Study of Cell Locomotion
Harris, A. K., Wild, P., & Stopak, D. (1980) · Science · doi:10.1126/science.6987736 · Cited by 1,348
Stresses at the Cell-to-Substrate Interface during Locomotion of Fibroblasts
Dembo, M., & Wang, Y.-L. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)77386-8 · Cited by 1,287
Scaling the Microrheology of Living Cells
Fabry, B., Maksym, G. N., Butler, J. P., Glogauer, M., Navajas, D., & Fredberg, J. J. (2001) · Physical Review Letters · doi:10.1103/physrevlett.87.148102 · Cited by 1,261
Self-citationExtracellular Matrix Rigidity Causes Strengthening of Integrin–Cytoskeleton Linkages
Choquet, D., Felsenfeld, D. P., & Sheetz, M. P. (1997) · Cell · doi:10.1016/s0092-8674(00)81856-5 · Cited by 1,245
Traction fields, moments, and strain energy that cells exert on their surroundings
Butler, J. P., Tolić-Nørrelykke, I. M., Fabry, B., & Fredberg, J. J. (2002) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00270.2001 · Cited by 1,043
Self-citationNonequilibrium Mechanics of Active Cytoskeletal Networks
Mizuno, D., Tardin, C., Schmidt, C. F., & MacKintosh, F. C. (2007) · Science · doi:10.1126/science.1134404 · Cited by 951
Force mapping in epithelial cell migration
du Roure, O., Saez, A., Buguin, A., Austin, R. H., Chavrier, P., Siberzan, P., & Ladoux, B. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408482102 · Cited by 779
Cited by
Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
Huang, G., Li, F., Zhao, X., Ma, Y., Li, Y., Lin, M., Jin, G., Lu, T. J., Genin, G. M., & Xu, F. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00094 · Cited by 825
Tensegrity, cellular biophysics, and the mechanics of living systems
Ingber, D. E., Wang, N., & Stamenović, D. (2014) · Reports on Progress in Physics · doi:10.1088/0034-4885/77/4/046603 · Cited by 485
Linear and Nonlinear Rheology of Living Cells
Kollmannsberger, P., & Fabry, B. (2011) · Annual Review of Materials Research · doi:10.1146/annurev-matsci-062910-100351 · Cited by 420
Focal adhesions, stress fibers and mechanical tension
Burridge, K., & Guilluy, C. (2016) · Experimental Cell Research · doi:10.1016/j.yexcr.2015.10.029 · Cited by 409
Investigating cell mechanics with atomic force microscopy
Haase, K., & Pelling, A. E. (2015) · Journal of The Royal Society Interface · doi:10.1098/rsif.2014.0970 · Cited by 396
Metadata
- Authors
- Krishnan, R., Park, C. Y., Lin, Y.-C., Mead, J., Jaspers, R. T., Trepat, X., Lenormand, G., Tambe, D., Smolensky, A. V., Knoll, A. H., Butler, J. P., & Fredberg, J. J.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2009-05-08
- Volume
- 4
- Issue
- 5
- Pages
- e5486
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2113123074
- Cited by
- 263
- References
- 33
- FWCI
- 7.92
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Reinforcement versus Fluidization in Cytoskeletal Mechanoresponsiveness. https://app.cochat.ai/papers/10.1371/journal.pone.0005486
- The paper
- Krishnan, R., Park, C. Y., Lin, Y.-C., Mead, J., Jaspers, R. T., Trepat, X., Lenormand, G., Tambe, D., Smolensky, A. V., Knoll, A. H., Butler, J. P., & Fredberg, J. J. (2009). Reinforcement versus Fluidization in Cytoskeletal Mechanoresponsiveness. PLoS ONE. https://doi.org/10.1371/journal.pone.0005486
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
