Linear dependence of muscle phosphocreatine kinetics on total creatine content
Abstract
Phosphorus nuclear magnetic resonance (NMR) spectra and twitch tension were recorded during stimulation of gastrocnemius muscles of pentobarbital sodium-anesthetized rats which had been fed the creatine analogue beta-guanidinopropionic acid (beta-GPA, 2% diet) for periods from 0 (control) to 8 wk. Total creatine content of unstimulated muscles decreased by 42, 67, 82, and 88% compared with controls after 2-, 4-, 6-, and 8-wk feeding, respectively. The staircase effect observed in control muscles during 8 min of twitch stimulation at 0.25, 0.5, and 0.75 Hz was reduced after 2- to 8-wk beta-GPA feeding. However, after 6- to 8-wk feeding, the twitch force at the end of 8 min of stimulation was not different from controls. The time constant for phosphocreatine (PCr) changes at the onset of and during recovery after stimulation was proportional to total creatine content. The relationship between PCr content and twitch rate times force at the end of stimulation was linear, with slope proportional to total creatine content. PCr content in beta-GPA-fed animals was transiently greater during recovery than before stimulation, suggesting a regulatory effect of the inorganic phosphate released by hydrolysis of phosphorylated beta-GPA. The results are consistent with linear models of respiratory control in which the creatine kinase reaction acts as a simple buffer of adenine nucleotide levels.
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A lower ATP cost of force production would blunt the changes in intramuscular substrates and metabolites that stimulate mitochondrial respiration (e.g. PCr, ADP, Pi).
“A lower ATP cost of force production would blunt the changes in intramuscular substrates and metabolites that stimulate mitochondrial respiration (e.g. PCr, ADP, Pi) [67, 68], and could explain the lower oxygen cost of exercise.”
From Dietary Nitrate Supplementation and Exercise Performance (2014) · cites this paper as [68]
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- Authors
- Meyer, R. A.
- Affiliations
- Michigan State University (US)
- Corresponding author
- Meyer, R. A.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 1989-12-01
- Volume
- 257
- Issue
- 6
- Pages
- C1149-C1157
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2472778196
- Cited by
- 103
- References
- 12
- Citations by year
- 2026: 1 · 2023: 4 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 4 · 2016: 4 · 2015: 2 · 2014: 5 · 2013: 3 · 2012: 3
- FWCI
- 0.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR-38972)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- creatine content, beta-guanidinopropionic acid, gastrocnemius, staircase effect, twitch force, inorganic phosphate, creatine kinase, respiratory control
- MeSH terms
- Adenosine Diphosphate, Adenosine Triphosphate, Animals, Creatine, Electric Stimulation, Guanidines, Hydrogen-Ion Concentration, Kinetics, Male, Muscles, Magnetic Resonance Spectroscopy, Phosphocreatine, Propionates, Rats, Inbred Strains, Reference Values, Time Factors, Rats
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