Cited by 162Trauma, Hemostasis, Coagulopathy, ResuscitationHormonal and reproductive studiesAdrenal Hormones and Disorders

Male sex steroids are responsible for depressing macrophage immune function after trauma-hemorrhage

Wichmann, Matthias W., Ayala, Alfred, Chaudry, Irshad H.·1997·American Journal of Physiology-Cell Physiology

Abstract

Recent studies suggest beneficial effects of castration before soft tissue trauma and hemorrhagic shock on splenocyte immune functions. Nonetheless, it remains unknown whether this effect of testosterone depletion is limited to splenocytes or is a generalized effect on immune function. The present study was therefore carried out to determine whether androgen depletion before trauma-hemorrhage also has salutary effects on splenic and peritoneal macrophage as well as on Kupffer cell function, as indicated by interleukin (IL)-1 and IL-6 release. Male C3H/HeN mice were castrated or sham-castrated 2 wk before the experiment and were killed at 24 h after trauma-hemorrhage and resuscitation. Significant depression of macrophage IL-1 and IL-6 release was only observed in sham-castrated mice, as opposed to normal levels of cytokine release from castrated animals after trauma-hemorrhage. In addition, only sham-castrated animals showed significantly increased levels of IL-6 release from Kupffer cells, which is believed to contribute to the systemic inflammatory response to trauma-hemorrhage. These observations suggest that the beneficial effects of androgen depletion before trauma-hemorrhage are not limited to splenocyte immune functions but are more global in nature. These results in surgically castrated animals suggest that androgen-blocking agents should be studied for their potential to reverse the immunodepression associated with trauma-hemorrhage.

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Crossref · 10.1152/ajpcell.1997.273.4.c1335

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Authors
Wichmann, M. W., Ayala, A., & Chaudry, I. H.
Affiliations
Brown University (US)
Venue
American Journal of Physiology-Cell Physiology
Published
1997-10-01
Volume
273
Issue
4
Pages
C1335-C1340
ISSN
  • 0363-6143 (ISSN-L)
  • 1522-1563
OpenAlex
W2243947562
Cited by
162
References
25
Citations by year
2026: 2 · 2025: 4 · 2023: 3 · 2022: 4 · 2021: 2 · 2020: 5 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 2 · 2014: 3 · 2013: 4 · 2012: 6
FWCI
9.50
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Cell Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (R01-GM37127, R01GM037127)
Topics
  • Health Sciences › Medicine › Critical Care and Intensive Care Medicine › Trauma, Hemostasis, Coagulopathy, Resuscitation
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
Keywords
traumatic hemorrhage, castration, testosterone, macrophage function, Kupffer cells, splenic macrophages, peritoneal macrophages, interleukin-1, interleukin-6, immunodepression, systemic inflammatory response, hemorrhagic shock
MeSH terms
Androgens, Animals, Cell Line, Cells, Cultured, Hemorrhage, Interleukin-1, Kupffer Cells, Macrophages, Male, Mice, Inbred C3H, Orchiectomy, Spleen, Wounds and Injuries, Interleukin-6, Macrophages, Peritoneal, Mice

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