The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Apelin is necessary for the maintenance of insulin sensitivity
Open accessAbstract
The recently discovered peptide apelin is known to be involved in the maintenance of insulin sensitivity. However, questions persist regarding its precise role in the chronic setting. Fasting glucose, insulin, and adiponectin levels were determined on mice with generalized deficiency of apelin (APKO). Additionally, insulin (ITT) and glucose tolerance tests (GTT) were performed. To assess the impact of exogenously delivered apelin on insulin sensitivity, osmotic pumps containing pyroglutamated apelin-13 or saline were implanted in APKO mice for 4 wk. Following the infusion, ITT/GTTs were repeated and the animals euthanized. Soleus muscles were harvested and homogenized in lysis buffer, and insulin-induced Akt phosphorylation was determined by Western blotting. Apelin-13 infusion and ITTs/GTTs were also performed in obese diabetic db/db mice. To probe the underlying mechanism for apelin's effects, apelin-13 was also delivered to cultured C 2 C 12 myotubes. 2-[3 H]deoxyglucose uptake and Akt phosphorylation were assessed in the presence of various inhibitors. APKO mice had diminished insulin sensitivity, were hyperinsulinemic, and had decreased adiponectin levels. Soleus lysates had decreased insulin-induced Akt phosphorylation. Administration of apelin to APKO and db/db mice resulted in improved insulin sensitivity. In C 2 C 12 myotubes, apelin increased glucose uptake and Akt phosphorylation. These events were fully abrogated by pertussis toxin, compound C, and siRNA knockdown of AMPKα1 but only partially diminished by LY-294002 and not at all by l-NAME. We conclude that apelin is necessary for the maintenance of insulin sensitivity in vivo. Apelin's effects on glucose uptake and Akt phosphorylation are in part mediated by a G i and AMPK-dependent pathway.
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Cites
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The exerkine apelin reverses age-associated sarcopenia
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The apelin receptor APJ: journey from an orphan to a multifaceted regulator of homeostasis
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Metadata
- Authors
- Yue, P., Jin, H., Aillaud, M., Deng, A. C., Azuma, J., Asagami, T., Kundu, R. K., Reaven, G. M., Quertermous, T., & Tsao, P. S.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2010-01
- Volume
- 298
- Issue
- 1
- Pages
- E59-E67
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2096979174
- Cited by
- 277
- References
- 44
- FWCI
- 9.82
- Type
- journal article
- Language
- en
- Open access
- green
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- Yue, P., Jin, H., Aillaud, M., Deng, A. C., Azuma, J., Asagami, T., Kundu, R. K., Reaven, G. M., Quertermous, T., & Tsao, P. S. (2010). Apelin is necessary for the maintenance of insulin sensitivity. American Journal of Physiology-Endocrinology and Metabolism. https://doi.org/10.1152/ajpendo.00385.2009
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