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Cited by 143Muscle metabolism and nutritionMuscle Physiology and DisordersCardiovascular and exercise physiology

The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.

Activation of mTORC1 signaling and protein synthesis in human muscle following blood flow restriction exercise is inhibited by rapamycin

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Gundermann, David M., Walker, Dillon K., Reidy, Paul T., Borack, Michael S., Dickinson, Jared M., Volpi, Elena, Rasmussen, Blake B.·2014·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00600.2013PMC PMC4116405
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Abstract

Restriction of blood flow to a contracting muscle during low-intensity resistance exercise (BFR exercise) stimulates mTORC1 signaling and protein synthesis in human muscle within 3 h postexercise. However, there is a lack of mechanistic data to provide a direct link between mTORC1 activation and protein synthesis in human skeletal muscle following BFR exercise. Therefore, the primary purpose of this study was to determine whether mTORC1 signaling is necessary for stimulating muscle protein synthesis after BFR exercise. A secondary aim was to describe the 24-h time course response in muscle protein synthesis and breakdown following BFR exercise. Sixteen healthy young men were randomized to one of two groups. Both the control (CON) and rapamycin (RAP) groups completed BFR exercise; however, RAP was administered 16 mg of the mTOR inhibitor rapamycin 1 h prior to BFR exercise. BFR exercise consisted of four sets of leg extension exercise at 20% of 1 RM. Muscle biopsies were collected from the vastus lateralis before exercise and at 3, 6, and 24 h after BFR exercise. Mixed-muscle protein fractional synthetic rate increased by 42% at 3 h postexercise and 69% at 24 h postexercise in CON, whereas this increase was inhibited in the RAP group. Phosphorylation of mTOR (Ser(2448)) and S6K1 (Thr(389)) was also increased in CON but inhibited in RAP. Mixed-muscle protein breakdown was not significantly different across time or groups. We conclude that activation of mTORC1 signaling and protein synthesis in human muscle following BFR exercise is inhibited in the presence of rapamycin.

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Crossref · 10.1152/ajpendo.00600.2013

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  1. Abe T, 2010, J Geriatr Phys Ther, 33, 34
  2. Abe T, 2004, Med Sci Sports Exerc, 36, S353
  3. Yokokawa Y, 2008, Biosci Trends, 2, 117

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Metadata

Authors
Gundermann, D. M., Walker, D. K., Reidy, P. T., Borack, M. S., Dickinson, J. M., Volpi, E., & Rasmussen, B. B.
Affiliations
  • Department of Nutrition and Metabolism,
  • The University of Texas Medical Branch at Galveston (US)
Corresponding author
Rasmussen, Blake B.
ORCID
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2014-05-15
Volume
306
Issue
10
Pages
E1198-E1204
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.00600.2013
PMC
PMC4116405
OpenAlex
W2140240000
Cited by
143
References
40
Citations by year
2026: 11 · 2025: 13 · 2024: 8 · 2023: 17 · 2022: 13 · 2021: 10 · 2020: 14 · 2019: 12 · 2018: 12 · 2017: 8 · 2016: 11 · 2015: 12 · 2014: 2
FWCI
5.48
Type
journal article
Language
en
Open access
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Institute of Clinical and Translational Sciences
  • National Institutes of Health
  • National Institute on Aging (P30AG024832)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases
  • National Institute of Child Health and Human Development
  • National Center for Advancing Translational Sciences
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Physiology (medical) › Cardiovascular and exercise physiology
Keywords
blood flow restriction exercise, mTORC1 signaling, muscle protein synthesis, rapamycin, muscle protein degradation, skeletal muscle, S6K1 phosphorylation, mTOR phosphorylation, vastus lateralis, resistance training, leg extension
MeSH terms
Mechanistic Target of Rapamycin Complex 1, Adolescent, Adult, Constriction, Humans, Male, Muscle Contraction, Regional Blood Flow, Protein Biosynthesis, Signal Transduction, Exercise, Muscle, Skeletal, Sirolimus, Multiprotein Complexes, Young Adult, TOR Serine-Threonine Kinases

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