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Cited by 71Cardiovascular and exercise physiologyMuscle metabolism and nutritionMetabolism, Diabetes, and Cancer

Control of glycolysis in contracting skeletal muscle. I. Turning it on

Crowther, Gregory J., Carey, Michael F., Kemper, William F., Conley, Kevin E.·2002·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.2002.282.1.e67
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Abstract

Why does the onset of glycolytic flux in muscle lag the start of exercise? We tested the hypothesis that both elevated metabolite levels and muscle activity are required for flux to begin. Glycolytic flux was determined from changes in muscle pH, phosphocreatine concentration, and P i concentration ([P i]) as measured by 31 P magnetic resonance spectroscopy. Eight subjects performed rapid ankle dorsiflexions to ∼45% of maximal voluntary contraction force under ischemia at a rate of 1 contraction/s. Subjects completed two bouts of exercise separated by 1 min of ischemic rest. Glycolytic flux was activated by 27 s in the first bout, ceased during the ischemic rest period, and was activated more quickly in the second bout. Because the onset in both bouts occurred at approximately the same [P i], ADP concentration, and AMP concentration, the activation of glycolysis appears to be related to the elevation of these metabolite concentrations. However, because no glycolytic flux occurred at rest, even when metabolite levels were high, both muscle activity and elevated metabolites are needed to turn on this pathway. We conclude that the delayed onset of glycolytic flux during exercise reflects the time needed to raise metabolites to flux-activating levels.

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  • Sprint exercises rapidly deplete intramuscular phosphocreatine (PCr) and increase inorganic phosphate levels, which then activate anaerobic glycolysis.

    “Sprint exercises rapidly deplete intramuscular phosphocreatine (PCr) and increase inorganic phosphate levels, which then activate anaerobic glycolysis31.”

    From Evaluating bioenergetic pathway contributions from single to multiple sprints (2024) · cites this paper as [31]

  • The capacity for PCr resynthesis, muscular buffering mechanisms, and aerobic fitness can help delay the negative effects of glycolytic by-products such as H+ accumulation and lactate.

    “The capacity for PCr resynthesis, muscular buffering mechanisms, and aerobic fitness can help delay the negative effects of glycolytic by-products such as H+accumulation and lactate16,18,31.”

    From Evaluating bioenergetic pathway contributions from single to multiple sprints (2024) · cites this paper as [31]

  • Glycolytic ATP production enhances peak sprint performance during early sprints.

    “Despite this, glycolytic ATP production is known to enhance peak sprint performance during early sprints, which highlights the paradox of whether maximal glycolytic activity improves overall RSA performance20,31,36.”

    From Evaluating bioenergetic pathway contributions from single to multiple sprints (2024) · cites this paper as [31]

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Authors
Crowther, G. J., Carey, M. F., Kemper, W. F., & Conley, K. E.
Affiliations
  • Physiology and Biophysics, and
  • Departments of Radiology,
  • University of Washington Medical Center (US)
ORCID
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2002-01-01
Volume
282
Issue
1
Pages
E67-E73
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.2002.282.1.e67
OpenAlex
W4230107734
Cited by
71
References
35
Citations by year
2026: 1 · 2025: 3 · 2024: 3 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 2 · 2016: 1 · 2015: 5 · 2014: 1 · 2013: 1 · 2012: 6
FWCI
12.17
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01ar045184-05)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR41928, AR-45184, R01AR041928)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords
glycolysis, skeletal muscle, exercise onset, glycolytic flux, muscle ischemia, phosphocreatine, inorganic phosphate, muscle pH, ADP, AMP, muscle activity, 31P NMR, ankle dorsiflexion
MeSH terms
Adenosine Triphosphate, Adult, Glycogen, Glycolysis, Humans, Male, Middle Aged, Muscle Contraction, Magnetic Resonance Spectroscopy, Phosphocreatine, Phosphorus, Exercise, Muscle, Skeletal

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