Corticotropin-releasing hormone stimulates SGK-1 kinase expression in cultured hippocampal neurons via CRH-R1
Abstract
Corticotropin-releasing hormone (CRH) has been shown to exhibit various functions in hippocampus. In the present study, we examined the effect of CRH on the expression of serum/glucocorticoid-inducible protein kinase-1 (SGK-1), a novel protein kinase, in primary cultured hippocampal neurons. A dose-dependent increase in mRNA and protein levels of SGK-1 as well as frequency of SGK-1-positive neurons occurred upon exposure to CRH (1 pmol/l to 10 nmol/l). These effects can be reversed by the specific CRH-R1 antagonist antalarmin but not by the CRH-R2 antagonist astressin 2B. Blocking adenylate cyclase (AC) activity with SQ22536 and PKA with H89 completely prevented CRH-induced mRNA and protein expression of SGK-1. Blockage of PLC or PKC did not block CRH-induced SGK-1 expression. Our results suggest that CRH act on CRH-R1 to stimulate SGK-1 mRNA and protein expression in cultured hippocampal neurons via a mechanism that is involved in AC/PKA signaling pathways.
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Cerebral SGK1 is upregulated by glucocorticoids, corticotropin releasing hormone, hyperactivity, intracranial self-stimulation, chronic escalating morphine regimen, and clozapine.
“Cerebral SGK1 is upregulated by glucocorticoids (Sato et al. 2008;Sarabdjitsingh et al. 2010), corticotropin releasing hormone (Sheng et al. 2008), hyperactivity (Kalinichev et al. 2008), intracranial self-stimulation (Huguet et al. 2009), chronic escalating morphine regimen (Befort et al. 2008) and administration of the antipsychotic drug clozapine (Robbins et al. 2008).”
From Significance of SGK1 in the regulation of neuronal function (2010) · cites this paper as Sheng et al. 2008
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Significance of SGK1 in the regulation of neuronal function
Lang, F., Strutz-Seebohm, N., Seebohm, G., & Lang, U. E. (2010) · The Journal of Physiology · doi:10.1113/jphysiol.2010.190926 · Cited by 170 · see citing works
Evidence for the role of corticotropin-releasing factor in major depressive disorder
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Molecular and cell signaling targets for PTSD pathophysiology and pharmacotherapy
Hauger, R. L., Olivares-Reyes, J. A., Dautzenberg, F. M., Lohr, J. B., Braun, S., & Oakley, R. H. (2012) · Neuropharmacology · doi:10.1016/j.neuropharm.2011.11.007 · Cited by 71 · see citing works
- Authors
- Sheng, H., Sun, T., Cong, B., He, P., Zhang, Y., Yan, J., Lu, C., & Ni, X.
- Affiliations
- Second Military Medical University (CN)
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2008-10
- Volume
- 295
- Issue
- 4
- Pages
- E938-E946
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2118909779
- Cited by
- 21
- References
- 3
- Citations by year
- 2024: 1 · 2022: 1 · 2020: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 3
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- corticotropin-releasing factor, SGK1, CRH receptor 1, hippocampal neurons, antalarmin, adenylate cyclase, protein kinase A, gene expression regulation, stress signaling
- MeSH terms
- CRF Receptor, Type 1, Serum-Glucocorticoid Regulated Kinases, Cyclic AMP, Animals, Animals, Newborn, Cells, Cultured, Corticotropin-Releasing Hormone, Enzyme Inhibitors, Fluorescent Antibody Technique, Hippocampus, Neurons, Protein Kinase C, RNA, RNA, Messenger, Stimulation, Chemical, Blotting, Western, Rats, Sprague-Dawley, Protein Serine-Threonine Kinases, Cyclic AMP-Dependent Protein Kinases, Immediate-Early Proteins, Receptors, Corticotropin-Releasing Hormone, Reverse Transcriptase Polymerase Chain Reaction, Rats, Phospholipase C beta
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