Cited by 60Neuropeptides and Animal PhysiologyGastrointestinal motility and disordersRegulation of Appetite and Obesity

Urocortin 2 Acts Centrally to Delay Gastric Emptying through Sympathetic Pathways while CRF and Urocortin 1 Inhibitory Actions Are Vagal Dependent in Rats

Czimmer, József, Million, Mulugeta, Taché, Yvette·2006·American Journal of Physiology-Gastrointestinal and Liver Physiology

Abstract

We characterized the influence of the selective corticotropin-releasing factor 2 (CRF(2)) receptor agonist human urocortin 2 (Ucn 2), injected intracisternally, on gastric emptying and its mechanism of action compared with intracisternal CRF or urocortin (Ucn 1) in conscious rats. The methylcellulose phenol red solution was gavaged 20 min after peptide injection, and gastric emptying was measured 20 min later. The intracisternal injection of Ucn 2 (0.1 and 1 microg) and Ucn 1 (1 microg) decreased gastric emptying to 37.8 +/- 6.9%, 23.1 +/- 8.6%, and 21.6 +/- 5.9%, respectively, compared with 58.4 +/- 3.8% after intracisternal vehicle. At lower doses, Ucn 2 (0.03 microg) and Ucn 1 (0.1 microg) had no effect. The CRF(2) antagonist astressin(2)-B (3 microg ic) antagonized intracisternal Ucn 2 (0.1 microg) and CRF (0.3 microg)-induced inhibition of gastric emptying. Vagotomy enhanced intracisternal Ucn 2 (0.1 or 1 microg)-induced inhibition of gastric emptying compared with sham-operated group, whereas it blocked intracisternal CRF (1 microg) inhibitory action (45.5 +/- 8.4% vs. 9.7 +/- 9.7%). Sympathetic blockade by bretylium prevented intracisternal and intracerebroventricular Ucn 2-induced delayed gastric emptying, whereas it did not influence intravenous Ucn 2-, intracisternal CRF-, and intracisternal Ucn 1-induced inhibition of gastric emptying. Prazosin abolished the intracisternal Ucn 2 inhibitory effect, whereas yohimbine and propranolol did not. None of the pretreatments modified basal gastric emptying. These data indicate that intracisternal Ucn 2 induced a central CRF(2)-mediated inhibition of gastric emptying involving sympathetic alpha(1)-adrenergic mechanisms independent from the vagus contrasting with the vagal-dependent inhibitory actions of CRF and Ucn 1.

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Crossref · 10.1152/ajpgi.00289.2005

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  • In the hypothalamus, PFOS caused upregulation of urocortin 2, an endogenous ligand for CRFR2 that suppresses feeding behavior and inhibits gastric emptying.

    “In the hypothalamus, PFOS caused upregulation of urocortin 2, an endogenous ligand for corticotropin releasing factor receptor 2 (CRFR2) that has been shown to suppress feeding behavior and inhibit gastric emptying”

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Authors
Czimmer, J., Million, M., & Taché, Y.
Affiliations
University of California, Los Angeles (US)
Venue
American Journal of Physiology-Gastrointestinal and Liver Physiology
Published
2006-03
Volume
290
Issue
3
Pages
G511-G518
ISSN
  • 0193-1857 (ISSN-L)
  • 1522-1547
OpenAlex
W2056625607
Cited by
59
References
52
Citations by year
2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 4 · 2019: 1 · 2018: 5 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 3 · 2012: 3
FWCI
1.56
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Gastrointestinal and Liver Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Diabetes and Digestive and Kidney Diseases (DK 41301, R01DK033061, R01 DK 33061, P30DK041301)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
Keywords
urocortin 2, urocortin 1, corticotropin-releasing factor, gastric emptying, vagus nerve, sympathetic nervous system, alpha1-adrenoceptors, vagotomy, prazosin, central nervous system, stress response, gastrointestinal motility, rats, sympathoinhibition
MeSH terms
Adrenergic alpha-Antagonists, Adrenergic beta-Antagonists, Animals, Bretylium Compounds, Corticotropin-Releasing Hormone, Gastric Emptying, Humans, Male, Peptide Fragments, Prazosin, Sympathetic Nervous System, Vagotomy, Vagus Nerve, Yohimbine, Rats, Urocortins

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