The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Role of glycocalyx in leukocyte-endothelial cell adhesion
Abstract
The binding of fluorescently labeled microspheres (FLMs, 0.1-μm diameter) coated with antibody (1a29) to ICAM-1 was studied in postcapillary venules during topical application of the chemoattractant N-formylmethionyl-leucyl-phenylalanine (fMLP). FLM adhesion to endothelial cells (ECs) increased dramatically from 50 to 150 spheres per 100-μm length of venule after superfusion of the mesentery with fMLP and equaled or exceeded levels of leukocyte (WBC) adhesion. Removal of the EC glycocalyx by micropipette infusion of the venule with heparinase increased FLM-EC adhesion to levels attained with fMLP. Subsequent application of fMLP did not increase FLM adhesion further, suggesting that the FLMs saturated all ICAM-1 binding sites. Perfusion with heparinase after suffusion with fMLP significantly increased FLM-EC adhesion above levels attained with fMLP. However, WBC adhesion fell because of possible removal of selectins necessary to maintain WBC rolling at the wall. It is concluded that the glycocalyx serves as a barrier to adhesion and that its shedding during natural activation of ECs may be an essential part of the inflammatory response.
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Cites
Adhesion receptors of the immune system
Springer, T. A. (1990) · Nature · doi:10.1038/346425a0 · Cited by 6,477
Giuffrè, L., Cordey, A.-S., Monai, N., Tardy, Y., Schapira, M., & Spertini, O. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.136.4.945 · Cited by 122
Cited by
Bergan, J. J., Schmid-Schönbein, G. W., Smith, P. D. C., Nicolaides, A. N., Boisseau, M. R., & Eklof, B. (2006) · New England Journal of Medicine · doi:10.1056/nejmra055289 · Cited by 933
Microvascular Rheology and Hemodynamics
LIPOWSKY, H. H. (2005) · Microcirculation · doi:10.1080/10739680590894966 · Cited by 414
Self-citationInvestigating cell mechanics with atomic force microscopy
Haase, K., & Pelling, A. E. (2015) · Journal of The Royal Society Interface · doi:10.1098/rsif.2014.0970 · Cited by 396
Metadata
- Authors
- Mulivor, A. W., & Lipowsky, H. H.
- Venue
- American Journal of Physiology-Heart and Circulatory Physiology
- Publisher
- American Physiological Society
- Published
- 2002-10-01
- Volume
- 283
- Issue
- 4
- Pages
- H1282-H1291
- ISSN
- 0363-6135 (ISSN-L)
- 1522-1539
- OpenAlex
- W2153870838
- Cited by
- 328
- References
- 2
- FWCI
- 3.69
- Type
- journal article
- Language
- en
- Open access
- closed
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- Mulivor, A. W., & Lipowsky, H. H. (2002). Role of glycocalyx in leukocyte-endothelial cell adhesion. American Journal of Physiology-Heart and Circulatory Physiology. https://doi.org/10.1152/ajpheart.00117.2002
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
