A method for assaying small amounts of antidiuretic substances with notes on some properties of vasopressin
Abstract
An intravenous method of high precision for bioassay of antidiuretic substances at low concentrations is described. Sensitivity is high and occurrence of nonspecific antidiuresis is largely avoided. It uses the rat, maintained under constant water load and ethanol anesthesia. Antidiuretic activity of commercial vasopressin in solution, studied under varying conditions of temperature and pH, withstood short boiling at any pH but was reduced after longer boiling, especially in alkaline solutions. Loss of pressor activity did not parallel that of antidiuretic activity at all pH levels. Vasopressin solutions lyophilized or frozen for long periods lost no antidiuretic activity. There was appreciable loss of activity on standing at room temperature for 72 hours. Plasma samples containing vasopressin may be acidified, boiled and centrifuged while the antidiuretic activity remains intact in the supernatant fluid.
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The antidiuretic bioassay was capable of high sensitivity and precision.
“The antidiuretic bioassay was capable of high sensitivity and precision”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [29]
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“By the late 1950s, antidiuretic assays could measure vaso-pressin at doses as low as 4 l U (10 pg) (29), a sensitivity achieved by injecting rats with alcohol to suppress endogenous vasopressin secretion and by maintaining a constant water load to stabilise the preparation for repeated measurements.”
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- Authors
- Dettelbach, H. R.
- Affiliations
- From the Division of Biological Research, G. D. Searle & Company, Chicago, Illinois
- Corresponding author
- Dettelbach, Harold R.
- Venue
- American Journal of Physiology-Legacy Content
- Publisher
- American Physiological Society
- Published
- 1958-01-31
- Volume
- 192
- Issue
- 2
- Pages
- 379-386
- ISSN
- 0002-9513 (ISSN-L)
- OpenAlex
- W2264868047
- Cited by
- 33
- References
- 0
- Citations by year
- 2016: 1 · 2015: 1
- FWCI
- 6.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Legacy Content (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Electrolyte and hormonal disorders
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- vasopressin, bioassay, antidiuretic activity, pressor activity, pH and temperature effects, thermal stability, freeze-drying, plasma samples, water load
- MeSH terms
- Arginine Vasopressin, Biological Assay, Humans, Vasopressins
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