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Cited by 331

The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.

Hypoxia induces type II NOS gene expression in pulmonary artery endothelial cells via HIF-1

Palmer, Lisa A., Semenza, Gregg L., Stoler, Mark H., Johns, Roger A.·1998·American Journal of Physiology-Lung Cellular and Molecular Physiology
DOI 10.1152/ajplung.1998.274.2.l212
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Abstract

Type II nitric oxide synthase (NOS) is upregulated in the pulmonary vasculature in a chronic hypoxia model of pulmonary hypertension. In situ hybridization analysis demonstrates that type II NOS RNA is increased in the endothelium as well as in the vascular smooth muscle in the lung. The current studies examine the role of hypoxia-inducible factor (HIF)-1 in regulating type II NOS gene expression in response to hypoxia in pulmonary artery endothelial cells. Northern blot analyses demonstrate a twofold increase in HIF-1α but not in HIF-1β RNA with hypoxia in vivo and in vitro. Electrophoretic mobility shift assays show the induction of specific DNA binding activity when endothelial cells were subjected to hypoxia. This DNA binding complex was identified as HIF-1 using antibodies directed against HIF-1α and HIF-1β. Transient transfection of endothelial cells resulted in a 2.7-fold increase in type II NOS promoter activity in response to hypoxia compared with nonhypoxic controls. Mutation or deletion of the HIF-1 site eliminated the response to hypoxia. These results demonstrate that HIF-1 is essential for the hypoxic regulation of type II NOS gene transcription in pulmonary endothelium.

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Metadata

Authors
Palmer, L. A., Semenza, G. L., Stoler, M. H., & Johns, R. A.
Venue
American Journal of Physiology-Lung Cellular and Molecular Physiology
Publisher
American Physiological Society
Published
1998-02-01
Volume
274
Issue
2
Pages
L212-L219
ISSN
  • 1040-0605 (ISSN-L)
  • 1522-1504
DOI
10.1152/ajplung.1998.274.2.l212
OpenAlex
W2140753483
Cited by
331
References
31
FWCI
19.51
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Hypoxia induces type II NOS gene expression in pulmonary artery endothelial cells via HIF-1. https://app.cochat.ai/papers/10.1152/ajplung.1998.274.2.l212
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Palmer, L. A., Semenza, G. L., Stoler, M. H., & Johns, R. A. (1998). Hypoxia induces type II NOS gene expression in pulmonary artery endothelial cells via HIF-1. American Journal of Physiology-Lung Cellular and Molecular Physiology. https://doi.org/10.1152/ajplung.1998.274.2.l212

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.