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Cited by 116Metabolism, Diabetes, and CancerDiabetes, Cardiovascular Risks, and LipoproteinsAdipose Tissue and Metabolism

The DOI matches its Crossref record.

Additive effect of contractions and insulin on GLUT-4 translocation into the sarcolemma

Gao, J., Ren, J., Gulve, E. A., Holloszy, J. O.·1994·Journal of Applied Physiology
DOI 10.1152/jappl.1994.77.4.1597
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Abstract

The maximal effects of insulin and muscle contractions on glucose transport are additive. GLUT-4 is the major glucose transporter isoform expressed in skeletal muscle. Muscle contraction and insulin each induce translocation of GLUT-4 from intracellular sites into the plasma membrane. The purpose of this study was to test the hypothesis that the incremental effect of contractions and insulin on glucose transport is mediated by additivity of the maximal effects of these stimuli on GLUT-4 translocation into the sarcolemma. Anesthetized rats were given insulin by intravenous infusion to raise plasma insulin to 2,635 +/- 638 microU/ml. The gastrocnemius-plantaris-soleus group was stimulated to contract via the sciatic nerve by using a protocol that maximally activates glucose transport. After treatment with insulin, contractions, or insulin plus contractions or no treatment, the gastrocnemius-plantaris-soleus muscle group was dissected out and was subjected to subcellular fractionation to separate the plasma membrane and intracellular membrane fractions. Insulin induced a 70% increase and contractions induced a 113% increase in the GLUT-4 content of the plasma membrane fraction. The effects of insulin and contractions were additive, as evidenced by a 185% increase in the GLUT-4 content of the sarcolemmal fraction. This finding provides evidence that the incremental effect of maximally effective insulin and contractile stimuli on glucose transport is mediated by additivity of their effects on GLUT-4 translocation into the sarcolemma.

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Metadata

Authors
Gao, J., Ren, J., Gulve, E. A., & Holloszy, J. O.
Affiliations
Washington University in St. Louis (US)
ORCID
Gao, J.
Venue
Journal of Applied Physiology
Publisher
American Physiological Society
Published
1994-10-01
Volume
77
Issue
4
Pages
1597-1601
ISSN
  • 0161-7567 (ISSN-L)
  • 8750-7587
  • 1522-1601
DOI
10.1152/jappl.1994.77.4.1597
OpenAlex
W1864159588
Cited by
116
References
0
Citations by year
2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 5 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 6 · 2015: 7 · 2014: 4 · 2013: 3 · 2012: 2
FWCI
1.52
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institute on Aging (AG 00078)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK20579, DK-18986, R01DK018986)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
  • Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
Keywords
GLUT4, glucose transporter translocation, skeletal muscle glucose transport, insulin, muscle contraction, additive effects, sarcolemma, plasma membrane, subcellular fractionation
MeSH terms
Animals, Cell Fractionation, Cell Membrane, Electrophoresis, Polyacrylamide Gel, Glucose, Insulin, Intracellular Membranes, Male, Monosaccharide Transport Proteins, Muscle Contraction, Muscle Proteins, Sarcolemma, Blotting, Western, Rats, Wistar, Muscle, Skeletal, Glucose Transporter Type 4, Rats

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