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Additive effect of contractions and insulin on GLUT-4 translocation into the sarcolemma
Abstract
The maximal effects of insulin and muscle contractions on glucose transport are additive. GLUT-4 is the major glucose transporter isoform expressed in skeletal muscle. Muscle contraction and insulin each induce translocation of GLUT-4 from intracellular sites into the plasma membrane. The purpose of this study was to test the hypothesis that the incremental effect of contractions and insulin on glucose transport is mediated by additivity of the maximal effects of these stimuli on GLUT-4 translocation into the sarcolemma. Anesthetized rats were given insulin by intravenous infusion to raise plasma insulin to 2,635 +/- 638 microU/ml. The gastrocnemius-plantaris-soleus group was stimulated to contract via the sciatic nerve by using a protocol that maximally activates glucose transport. After treatment with insulin, contractions, or insulin plus contractions or no treatment, the gastrocnemius-plantaris-soleus muscle group was dissected out and was subjected to subcellular fractionation to separate the plasma membrane and intracellular membrane fractions. Insulin induced a 70% increase and contractions induced a 113% increase in the GLUT-4 content of the plasma membrane fraction. The effects of insulin and contractions were additive, as evidenced by a 185% increase in the GLUT-4 content of the sarcolemmal fraction. This finding provides evidence that the incremental effect of maximally effective insulin and contractile stimuli on glucose transport is mediated by additivity of their effects on GLUT-4 translocation into the sarcolemma.
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“3) Insulin is additive to contraction in stimulating glucose uptake [109,110].”
From AMPK and Exercise: Glucose Uptake and Insulin Sensitivity (2013) · cites this paper as [110]
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Exercise, Glucose Transport, and Insulin Sensitivity
Goodyear, L. J., & Kahn, B. B. (1998) · Annual Review of Medicine · doi:10.1146/annurev.med.49.1.235 · Cited by 1,056
Cardiovascular Effects and Benefits of Exercise
Nystoriak, M. A., & Bhatnagar, A. (2018) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2018.00135 · Cited by 968
Invited Review: Effects of acute exercise and exercise training on insulin resistance
Henriksen, E. J. (2002) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01219.2001 · Cited by 535
“The translocation mechanism for increasing plasma membrane GLUT-4 also functions in response to contractions (37, 41), hypoxia (15), nitric oxide (33), and bradykinin (2, 107).”
BackgroundLund, S., Holman, G. D., Schmitz, O., & Pedersen, O. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.13.5817 · Cited by 492
Exercise regulation of glucose transport in skeletal muscle.
Hayashi, T., Wojtaszewski, J., & Goodyear, L. (1997) · American Journal of Physiology · doi:10.1152/ajpendo.1997.273.6.e1039 · Cited by 394
“Thus the results of two studies suggest that the increase in GLUT-4 at the plasma membrane is the sole mechanism for the increase in glucose transport (31, 75), whereas the two other studies raise the possibility that exercise increases glucose transporter turnover number (9, 23), a process that is frequently referred…”
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Metadata
- Authors
- Gao, J., Ren, J., Gulve, E. A., & Holloszy, J. O.
- Affiliations
- Washington University in St. Louis (US)
- ORCID
- Gao, J.
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 1994-10-01
- Volume
- 77
- Issue
- 4
- Pages
- 1597-1601
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W1864159588
- Cited by
- 116
- References
- 0
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 5 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 6 · 2015: 7 · 2014: 4 · 2013: 3 · 2012: 2
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (AG 00078)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK20579, DK-18986, R01DK018986)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Keywords
- GLUT4, glucose transporter translocation, skeletal muscle glucose transport, insulin, muscle contraction, additive effects, sarcolemma, plasma membrane, subcellular fractionation
- MeSH terms
- Animals, Cell Fractionation, Cell Membrane, Electrophoresis, Polyacrylamide Gel, Glucose, Insulin, Intracellular Membranes, Male, Monosaccharide Transport Proteins, Muscle Contraction, Muscle Proteins, Sarcolemma, Blotting, Western, Rats, Wistar, Muscle, Skeletal, Glucose Transporter Type 4, Rats
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