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Angiogenic growth factor mRNA responses in muscle to a single bout of exercise
Abstract
A major adaptation to exercise is new capillary formation in skeletal muscle. On the basis of angiogenesis in tumors and during development, several angiogenic growth factors may be involved, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and transforming growth factor-beta 1 (TGF-beta 1). In 9-wk-old female Wistar rats, mRNA expression for these three growth factors in gastrocnemius muscle was examined by quantitative Northern analysis after a single 1-h run at 15 or 20 m/min at 10 degrees incline in room air. A third group ran at 15 m/min in 12% O2, and resting control groups were included at inspired O2 fractions of 0.21 and 0.12. Exercise significantly increased mRNA levels two- to fourfold, which was evident over the first 4 h postexercise; by 8 and 24 h, mRNA levels returned to baseline. For all three factors, mRNA levels were significantly higher after exercise at 20 than at 15 m/min. Hypoxia at rest doubled VEGF and TGF-beta 1 message but had no effect on bFGF. Hypoxic exercise further raised VEGF mRNA levels but had no effect on the other factors. We suggest that VEGF, bFGF, and TGF-beta 1 may be involved in the angiogenic response to exercise and that reduced intracellular PO2 (as occurs during normoxic exercise) may be part of the stimulus to such growth factor production.
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“This paper demonstrated that a single bout of exercise can increase VEGF1 and TGFβ1 expression in muscle to promote the angiogenic response to exercise.”
From Exerkines in health, resilience and disease (2022) · cites this paper as [198]
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Role of Extracellular Matrix in Adaptation of Tendon and Skeletal Muscle to Mechanical Loading
Kjær, M. (2004) · Physiological Reviews · doi:10.1152/physrev.00031.2003 · Cited by 1,649
Exerkines in health, resilience and disease
Chow, L. S., Gerszten, R. E., Taylor, J. M., Pedersen, B. K., van Praag, H., Trappe, S., Febbraio, M. A., Galis, Z. S., Gao, Y., Haus, J. M., et al. (2022) · Nature Reviews Endocrinology · doi:10.1038/s41574-022-00641-2 · Cited by 927
Couffinhal, T., Silver, M., Zheng, L., Kearney, M., Witzenbichler, B., & Isner, J. M. (1998) · Europe PMC (PubMed Central) · Cited by 672
What makes vessels grow with exercise training?
Prior, B. M., Yang, H. T., & Terjung, R. L. (2004) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00035.2004 · Cited by 525
“The importance of hypoxia as an obvious candidate for the initiation of angiogenesis in active muscle during training was reinforced by data showing that hypoxia enhanced the exercise-induced upregulation of VEGF in rat muscle (10).”
Background
Metadata
- Authors
- Breen, E. C., Johnson, E. C., Wagner, H., Tseng, H. M., Sung, L. A., & Wagner, P. D.
- Affiliations
- University of California San Diego (US)
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 1996-07-01
- Volume
- 81
- Issue
- 1
- Pages
- 355-361
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W2137609684
- Cited by
- 302
- References
- 0
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 5 · 2021: 8 · 2020: 7 · 2019: 6 · 2018: 5 · 2017: 5 · 2016: 15 · 2015: 21 · 2014: 13 · 2013: 13 · 2012: 13
- FWCI
- 22.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl046910-04)
- National Heart, Lung, and Blood Institute (P01HL017731, 2-PO1-HL-17731, 5-RO1-HL-46910)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Cardiovascular and exercise physiology
- Health Sciences › Medicine › Physiology (medical) › Exercise and Physiological Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Keywords
- exercise-induced angiogenesis, angiogenic factors, vascular endothelial growth factor, basic fibroblast growth factor, TGF-β1, hypoxic exercise, hypoxia, intracellular oxygen tension, mRNA expression, gastrocnemius, single bout exercise
- MeSH terms
- Animals, Hypoxia, Physical Exertion, Female, Half-Life, RNA, Messenger, Blotting, Northern, Transforming Growth Factor beta, Fibroblast Growth Factor 2, Endothelial Growth Factors, Rats, Wistar, In Situ Hybridization, Muscle, Skeletal, Rats
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