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Cited by 123Nitric Oxide and Endothelin EffectsThermoregulation and physiological responsesCardiovascular and exercise physiology

New approach to measure cutaneous microvascular function: an improved test of NO-mediated vasodilation by thermal hyperemia

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Choi, Patricia J., Brunt, Vienna E., Fujii, Naoto, Minson, Christopher T.·2014·Journal of Applied Physiology
DOI 10.1152/japplphysiol.01397.2013PMC PMC4122693
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Abstract

Cutaneous hyperemia in response to rapid skin local heating to 42°C has been used extensively to assess microvascular function. However, the response is dependent on both nitric oxide (NO) and endothelial-derived hyperpolarizing factors (EDHFs), and increases cutaneous vascular conductance (CVC) to ∼90–95% maximum in healthy subjects, preventing the study of potential means to improve cutaneous function. We sought to identify an improved protocol for isolating NO-dependent dilation. We compared nine heating protocols (combinations of three target temperatures: 36°C, 39°C, and 42°C, and three rates of heating: 0.1°C/s, 0.1°C/10 s, 0.1°C/min) in order to select two protocols to study in more depth ( protocol 1; N = 6). Then, CVC was measured at four microdialysis sites receiving: 1) lactated Ringer solution (Control), 2) 50-mM tetraethylammonium (TEA) to inhibit EDHFs, 3) 20-mM nitro-L-arginine methyl ester (L-NAME) to inhibit NO synthase, and 4) TEA+L-NAME, in response to local heating either to 39°C at 0.1°C/s ( protocol 2; N = 10) or 42°C at 0.1°C/min ( protocol 3; N = 8). Rapid heating to 39°C increased CVC to 43.1 ± 5.2%CVCmax (Control), which was attenuated by L-NAME (11.4 ± 2.8%CVCmax; P < 0.001) such that 82.8 ± 4.2% of the plateau was attributable to NO. During gradual heating, 81.5 ± 3.3% of vasodilation was attributable to NO at 40°C, but at 42°C only 32.7 ± 7.8% of vasodilation was attributable to NO. TEA+L-NAME attenuated CVC beyond L-NAME at temperatures >40°C (43.4 ± 4.5%CVCmax at 42°C, P < 0.001 vs. L-NAME), suggesting a role of EDHFs at higher temperatures. Our findings suggest local heating to 39°C offers an improved approach for isolating NO-dependent dilation and/or assessing perturbations that may improve microvascular function.

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  • Impaired endothelium-dependent vasodilation results not only in impaired macrovascular function, but also in microvascular dysfunction, as measured by retinal artery vasodilation and heat-induced skin hyperemia.

    “Impaired endothelium-dependent vasodilation results not only in impaired macrovascular function, but also in microvascular dysfunction, as measured by retinal artery vasodilation and heat-induced skin hyperemia.”

    From The Vasculature in Prediabetes (2018) · cites this paper as [106]

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Authors
Choi, P. J., Brunt, V. E., Fujii, N., & Minson, C. T.
Affiliations
University of Oregon (US)
Corresponding author
Minson, Christopher T.
ORCID
Venue
Journal of Applied Physiology
Publisher
American Physiological Society
Published
2014-08-01
Volume
117
Issue
3
Pages
277-283
ISSN
  • 0161-7567 (ISSN-L)
  • 8750-7587
  • 1522-1601
DOI
10.1152/japplphysiol.01397.2013
PMC
PMC4122693
OpenAlex
W2128986918
Cited by
123
References
46
Citations by year
2026: 12 · 2025: 8 · 2024: 9 · 2023: 12 · 2022: 12 · 2021: 13 · 2020: 17 · 2019: 9 · 2018: 8 · 2017: 6 · 2016: 8 · 2015: 6 · 2014: 3
FWCI
5.11
Type
journal article
Language
en
Open access
Locations
  • Journal of Applied Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (HL081671, R01HL081671)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Health Sciences › Medicine › Physiology › Thermoregulation and physiological responses
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
Keywords
cutaneous microvascular function, nitric oxide, cutaneous vascular conductance, tetraethylammonium, L-NAME, NO synthase inhibition, microdialysis, vasodilation, heating rate, endothelium-dependent vasodilation
MeSH terms
Adult, Female, Hot Temperature, Humans, Hyperemia, Male, Nitric Oxide, Regional Blood Flow, Skin, Skin Physiological Phenomena, Vasodilation, Microdialysis, NG-Nitroarginine Methyl Ester, Microvessels, Young Adult

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