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Cited by 331Biomedical and Chemical ResearchCancer, Hypoxia, and MetabolismRedox biology and oxidative stress

The DOI matches its Crossref record; 684 of the 685 references it cites were found in a registry or an index.

Defining Physiological Normoxia for Improved Translation of Cell Physiology to Animal Models and Humans

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Keeley, Thomas P., Mann, Giovanni E.·2019·Physiological Reviews
DOI 10.1152/physrev.00041.2017
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Abstract

The extensive oxygen gradient between the air we breathe (Po 2 ~21 kPa) and its ultimate distribution within mitochondria (as low as ~0.5-1 kPa) is testament to the efforts expended in limiting its inherent toxicity. It has long been recognized that cell culture undertaken under room air conditions falls short of replicating this protection in vitro. Despite this, difficulty in accurately determining the appropriate O 2 levels in which to culture cells, coupled with a lack of the technology to replicate and maintain a physiological O 2 environment in vitro, has hindered addressing this issue thus far. In this review, we aim to address the current understanding of tissue Po 2 distribution in vivo and summarize the attempts made to replicate these conditions in vitro. The state-of-the-art techniques employed to accurately determine O 2 levels, as well as the issues associated with reproducing physiological O 2 levels in vitro, are also critically reviewed. We aim to provide the framework for researchers to undertake cell culture under O 2 levels relevant to specific tissues and organs. We envisage that this review will facilitate a paradigm shift, enabling translation of findings under physiological conditions in vitro to disease pathology and the design of novel therapeutics.

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Metadata

Authors
Keeley, T. P., & Mann, G. E.
Affiliations
King's College London (GB)
ORCID
Venue
Physiological Reviews
Publisher
American Physiological Society
Published
2019-01-01
Volume
99
Issue
1
Pages
161-234
ISSN
  • 0031-9333 (ISSN-L)
  • 1522-1210
DOI
10.1152/physrev.00041.2017
OpenAlex
W2804335534
Cited by
329
References
685
Citations by year
2026: 38 · 2025: 47 · 2024: 57 · 2023: 53 · 2022: 51 · 2021: 32 · 2020: 31 · 2019: 19
FWCI
4.62
Type
journal article
Language
en
Open access
Locations
  • Physiological Reviews (publishedVersion)
  • PubMed (publishedVersion)
  • Research Portal (King's College London) (submittedVersion, other-oa)
Funders
  • British Heart Foundation (BHF) (FS/13/66/30445, PG/13/1/29801)
  • Heart Research UK (NET RG2633)
Topics
  • Health Sciences › Medicine › Physiology (medical) › Biomedical and Chemical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
tissue oxygen tension, oxygen gradients, oxygen toxicity
MeSH terms
Air, Animals, Cell Physiological Phenomena, Humans, Mitochondria, Oxygen, Oxygen Consumption, Models, Animal

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