The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood
Open accessAbstract
Enrichment of rare circulating tumor cells (CTCs) in blood is typically achieved using antibodies to epithelial cell adhesion molecule (EpCAM), with detection using cytokeratin (CK) antibodies. However, EpCAM and CK are not expressed in some tumors and can be downregulated during epithelial-to-mesenchymal transition. A micro-fluidic system, not limited to EpCAM or CK, was developed to use multiple antibodies for capture followed by detection using CEE-Enhanced (CE), a novel in situ staining method that fluorescently labels the capture antibodies bound to CTCs. Higher recovery of CTCs was demonstrated using antibody mixtures compared to anti-EpCAM. In addition, CK-positive breast cancer cells were found in 15 of 24 samples (63%; range 1–60 CTCs), while all samples contained additional CE-positive cells (range 1–41; median = 11; ). Thus, antibody mixtures against a range of cell surface antigens enables capture of more CTCs than anti-EpCAM alone and CE staining enables the detection of CK-negative CTCs.
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Allard, W. J., Matera, J., Miller, M. C., Repollet, M., Connelly, M. C., Rao, C., Tibbe, A. G. J., Uhr, J. W., & Terstappen, L. W. M. M. (2004) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-04-0378 · Cited by 2,542
The human keratins: biology and pathology
Moll, R., Divo, M., & Langbein, L. (2008) · Histochemistry and Cell Biology · doi:10.1007/s00418-008-0435-6 · Cited by 1,465
Attard, G., Swennenhuis, J. F., Olmos, D., Reid, A. H. M., Vickers, E., A'Hern, R., Levink, R., Coumans, F., Moreira, J., Riisnaes, R., et al. (2009) · Cancer Research · doi:10.1158/0008-5472.can-08-3667 · Cited by 533
Cited by
Krebs, M. G., Hou, J.-M., Sloane, R., Lancashire, L., Priest, L., Nonaka, D., Ward, T. H., Backen, A., Clack, G., Hughes, A., et al. (2012) · Journal of Thoracic Oncology · doi:10.1097/jto.0b013e31823c5c16 · Cited by 463
Technologies for circulating tumor cell separation from whole blood
Bankó, P., Lee, S. Y., Nagygyörgy, V., Zrínyi, M., Chae, C. H., Cho, D. H., & Telekes, A. (2019) · Journal of Hematology & Oncology · doi:10.1186/s13045-019-0735-4 · Cited by 358
Ignatiadis, M., Lee, M., & Jeffrey, S. S. (2015) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-14-1190 · Cited by 355
Circulating tumor cell isolation for cancer diagnosis and prognosis
Deng, Z., Wu, S., Wang, Y., & Shi, D. (2022) · eBioMedicine · doi:10.1016/j.ebiom.2022.104237 · Cited by 284
Isolation of Circulating Tumor Cells by Dielectrophoresis
Gascoyne, P., & Shim, S. (2014) · Cancers · doi:10.3390/cancers6010545 · Cited by 275
Metadata
- Authors
- Mikolajczyk, S. D., Millar, L. S., Tsinberg, P., Coutts, S. M., Zomorrodi, M., Pham, T., Bischoff, F. Z., & Pircher, T. J.
- Venue
- Journal of Oncology
- Publisher
- Hindawi Limited
- Published
- 2011
- Volume
- 2011
- Pages
- 1-10
- ISSN
- 1687-8450 (ISSN-L)
- 1687-8469
- OpenAlex
- W2147244189
- Cited by
- 254
- References
- 3
- FWCI
- 11.67
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Mikolajczyk, S. D., Millar, L. S., Tsinberg, P., Coutts, S. M., Zomorrodi, M., Pham, T., Bischoff, F. Z., & Pircher, T. J. (2011). Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood. Journal of Oncology. https://doi.org/10.1155/2011/252361
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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