Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry
Open accessAbstract
Chlorella ellipsoidea and Chlorococcum infusionum, promising microalgae for biodiesel feedstock production, were treated with ethylenediaminetetraacetate (EDTA) and phosphorous to induce stress which was then followed by flow cytometry to study the enhanced intracellular neutral lipid content. Treatment resulted in up to a threefold increase in total lipid content of Chlorella (41.8±1.9% at 16 days of incubation period) and more than twofold increases in Chlorococcum (31.3±1.0% at 18 days of incubation period) under phosphorous starvation in the culture. It was observed that maximum biomass yields in Chlorella and Chlorococcum were 1.56±0.06 and 2.17±0.12 g/L at 1.5 g/L of phosphorous after 20 and 18 days of incubation periods, respectively. The qualitative analyses of neutral lipid bodies under stress conditions were performed by confocal microscopy and revealed bright golden-yellow lipid droplets in stress exposed cells. Significant increase of monounsaturated fatty acids under the nutrient limited conditions was suitable to produce biodiesel. The maximum biomass (g/L) and lipid content (% dry cell weight) at different stresses showed significant results (p<0.05) by single-factor Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT).
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Under phosphorus starvation, total lipid content increased threefold in Chloroidium ellipsoideum and twofold in Chlorococcum infusionum.
“For example, under phosphorus starvation, the content of total lipids increased threefold in Chloroidium ellipsoideum (CUH/Al/MW-189), and twofold in Chlorococcum infusionum (CUH/Al/MW-190) [42].”
From Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae (2023) · cites this paper as [42]
Section 3.2 reports increased total lipids under reduced phosphorus: 2.7-fold in Chlorella ellipsoidea (CUH/Al/MW-189) and 2.5-fold in Chlorococcum infusionum (CUH/Al/MW-190). The citing sentence captures the general increase but calls the first organism Chloroidium ellipsoideum, whereas section 2.1 names it Chlorella ellipsoidea. It also rounds the second increase down to twofold and describes both treatments as starvation, although Chlorella received 0.15 g/L phosphorus. (LLM, conf=0.88)
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“For example, under phosphorus starvation, the content of total lipids increased threefold in Chloroidium ellipsoideum (CUH/ Al/MW-189), and twofold in Chlorococcum infusionum (CUH/Al/MW-190) [42].”
Background
- Authors
- Satpati, G. G., Gorain, P. C., & Pal, R.
- Affiliations
- University of Calcutta (IN)
- Corresponding author
- Pal, Ruma
- Venue
- Advances in Biology
- Publisher
- Hindawi Limited
- Published
- 2016-01-17
- Volume
- 2016
- Pages
- 1-12
- ISSN
- 2314-7563 (ISSN-L)
- 2356-6582
- OpenAlex
- W2293374331
- Cited by
- 33
- References
- 41
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 4 · 2020: 5 · 2019: 5 · 2018: 2 · 2017: 1 · 2016: 2
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Status
- Retracted
- Update notices
- retraction (2026-06-08)
- Open access
- hybrid
- doi.org
- Locations
- Advances in Biology (publishedVersion, cc-by)
- Funders
- Department of Science and Technology (DST), New Delhi (DST/IS-STAC/CO2-SR-166/13(G))
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Chlorella ellipsoidea, microalgae, biodiesel feedstock, neutral lipid accumulation, phosphorus deficiency, phosphorus limitation, biomass yield, lipid droplets, monounsaturated fatty acids, confocal microscopy
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