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Cited by 29Pluripotent Stem Cells ResearchMesenchymal stem cell researchAnimal Genetics and Reproduction

Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues

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Pessôa, Laís Vicari de Figueiredo, Pires, Pedro Ratto Lisboa, del Collado, Maite, Pieri, Naira Caroline Godoy, Recchia, Kaiana, Souza, Aline Fernanda, Perecin, Felipe, da Silveira, Juliano Coelho +4 more·2019·Stem Cells International
DOI 10.1155/2019/1393791PMC PMC6525926
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Abstract

Introduction. Pluripotent stem cells are believed to have greater clinical potential than mesenchymal stem cells due to their ability to differentiate into almost any cell type of an organism, and since 2006, the generation of patient-specific induced pluripotent stem cells (iPSCs) has become possible in multiple species. Objectives. We hypothesize that different cell types respond differently to the reprogramming process; thus, the goals of this study were to isolate and characterize equine adult and fetal cells and induce these cells to pluripotency for future regenerative and translational purposes. Methods. Adult equine fibroblasts (eFibros) and mesenchymal cells derived from the bone marrow (eBMmsc), adipose tissue (eADmsc), and umbilical cord tissue (eUCmsc) were isolated, their multipotency was characterized, and the cells were induced in vitro into pluripotency (eiPSCs). eiPSCs were generated through a lentiviral system using the factors OCT4, SOX2, c-MYC, and KLF4. The morphology and in vitro pluripotency maintenance potential (alkaline phosphatase detection, embryoid body formation, in vitro spontaneous differentiation, and expression of pluripotency markers) of the eiPSCs were characterized. Additionally, a miRNA profile analysis of the mesenchymal and eiPSCs was performed. Results. Multipotent cells were successfully isolated, but the eBMmsc failed to generate eiPSCs. The eADmsc-, eUCmsc-, and eFibros-derived iPSCs were positive for alkaline phosphatase, OCT4 and NANOG, were exclusively dependent on bFGF, and formed embryoid bodies. The miRNA profile revealed a segregated pattern between the eiPSCs and multipotent controls: the levels of miR-302/367 and the miR-92 family were increased in the eiPSCs, while the levels of miR-23, miR-27, and miR-30, as well as the let-7 family were increased in the nonpluripotent cells. Conclusions. We were able to generate bFGF-dependent iPSCs from eADmsc, eUCmsc, and eFibros with human OSKM, and the miRNA profile revealed that clonal lines may respond differently to the reprogramming process.

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  • In horse cell reprogramming, miR-23 expression level was decreased in induced pluripotent stem cells, which can recognize breed characteristics of horses.

    “the expression level of miR-23 was decreased in induced pluripotent stem cells (iPSCs), which can recognize the breed characteristics of horses.”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [104]

    The Results (section 3.3.2; page not provided) report that miR-23a was downregulated in equine iPSCs derived from umbilical-cord cells relative to the source cells. The paper does not say that miR-23 expression can identify horse breed characteristics; its discussion of breed concerns possible differences in cell proliferation rates (section 4.1). (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • miR-23a and miR-23b share a common target and have consistent functions, affecting skeletal muscle differentiation by inhibiting TrxR1 and participating in skeletal muscle generation.

    “miR-23a and miR-23b share a common target and have consistent functions, which can affect skeletal muscle differentiation by inhibiting Thioredoxin reductase 1 (TrxR1) and participate in skeletal muscle generation [[104], [105], [106]].”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [104]

    The Results (section 3.3, page unavailable) report that equine miR-23a and miR-23b were both downregulated in induced pluripotent stem cells. Our copy does not address whether they share a target, inhibit TrxR1, or affect skeletal muscle differentiation; searches for TrxR1 and skeletal muscle differentiation found no supporting passage. (LLM, conf=0.72)

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Authors
Pessôa, L. V. D. F., Pires, P. R. L., del Collado, M., Pieri, N. C. G., Recchia, K., Souza, A. F., Perecin, F., da Silveira, J. C., de Andrade, A. F. C., Ambrosio, C. E., Bressan, F. F., & Meirelles, F. V.
Affiliations
  • Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga 13635-000, Brazil
  • Universidade de São Paulo (BR)
  • Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Pirassununga 13635-000, Brazil
ORCID
Venue
Stem Cells International
Publisher
Wiley
Published
2019-05-02
Volume
2019
Pages
1-15
ISSN
  • 1687-966X (ISSN-L)
  • 1687-9678
DOI
10.1155/2019/1393791
PMC
PMC6525926
OpenAlex
W2942759009
Cited by
29
References
3
Citations by year
2026: 3 · 2025: 5 · 2024: 5 · 2023: 4 · 2022: 2 · 2021: 4 · 2020: 5 · 2019: 1
FWCI
0.99
Type
journal article
Language
en
Open access
Licence
Locations
  • Stem Cells International (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (2013/08135-2, 23038.006964/2014-43, 2015/26818-5, 2014/22887-0, 2013/17955-3, 2012/50533-2, 2008/57877-3)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (2013/08135-2, 23038.006964/2014-43, 2015/26818-5, 2014/22887-0, 2013/17955-3, 2012/50533-2, 2008/57877-3)
  • CEPID-CTC (2013/08135-2, 23038.006964/2014-43, 2015/26818-5, 2014/22887-0, 2013/17955-3, 2012/50533-2, 2008/57877-3)
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (2013/08135-2, 23038.006964/2014-43, 2015/26818-5, 2014/22887-0, 2013/17955-3, 2012/50533-2, 2008/57877-3)
  • National Institute of Science and Technology in Cell Therapy (2013/08135-2, 23038.006964/2014-43, 2015/26818-5, 2014/22887-0, 2013/17955-3, 2012/50533-2, 2008/57877-3)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
Keywords
iPSC reprogramming, miRNA expression profiling, mesenchymal stem cells, adipose-derived stem cells, umbilical cord-derived stem cells, bone marrow mesenchymal stem cells, OSKM factors, Oct4, Nanog, embryoid body formation, stem cell markers

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