Induced Pluripotent Stem Cell Generation Using a Single Lentiviral Stem Cell Cassette
Open accessAbstract
Induced pluripotent stem (iPS) cells can be generated using retroviral vectors expressing Oct4, Klf4, Sox2, and cMyc. Most prior studies have required multiple retroviral vectors for reprogramming, resulting in high numbers of genomic integrations in iPS cells and limiting their use for therapeutic applications. Here we describe the use of a single lentiviral vector expressing a “stem cell cassette” composed of the four transcription factors and a combination of 2A peptide and internal ribosome entry site technology, generating iPS cells from postnatal fibroblasts. iPS cells generated in this manner display embryonic stem cell-like morphology, express stem cell markers, and exhibit in vivo pluripotency, as evidenced by their ability to differentiate in teratoma assays and their robust contribution to mouse chimeras. Combining all factors into a single transcript achieves the most efficient reprogramming system to date and allows derivation of iPS cells with a single viral integration. The use of a single lentiviral vector for reprogramming represents a powerful laboratory tool and a significant step toward the application of iPS technology for clinical purposes.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Polycistronic technology reduces the requirement for multiple viral vectors to deliver four different transcription factors to a single-stem cell cassette.
“Polycistronic technology reduces the requirement for multiple viral vectors to deliver four different transcription factors, cutting this down to a single-stem cell cassette [101].”
From Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through (2022) · cites this paper as [101]
Fibroblasts from four previously diagnosed FAP individuals were reprogrammed using the STEMCCA system to generate three independent iPSC lines.
“Fibroblasts from four previously diagnosed FAP individuals were obtained from Coriell and reprogrammed using the STEMCCA system to generate three independent iPSC lines, as described [12, 18]”
From Modeling APC mutagenesis and familial adenomatous polyposis using human iPS cells (2018) · cites this paper as [18]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673 · see citing works
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221 · see citing works
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310 · see citing works
Generation of germline-competent induced pluripotent stem cells
Okita, K., Ichisaka, T., & Yamanaka, S. (2007) · Nature · doi:10.1038/nature05934 · Cited by 4,295 · see citing works
Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
Mikkelsen, T. S., Ku, M., Jaffe, D. B., Issac, B., Lieberman, E., Giannoukos, G., Alvarez, P., Brockman, W., Kim, T.-K., Koche, R. P., et al. (2007) · Nature · doi:10.1038/nature06008 · Cited by 4,129 · see citing works
Niwa, H., Miyazaki, J., & Smith, A. G. (2000) · Nature Genetics · doi:10.1038/74199 · Cited by 3,606 · see citing works
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
Wernig, M., Meissner, A., Foreman, R. K., Brambrink, T., Ku, M., Hochedlinger, K., Bernstein, B., & Jaenisch, R. (2007) · Nature · doi:10.1038/nature05944 · Cited by 2,669 · see citing works
Self-citationGenome-scale DNA methylation maps of pluripotent and differentiated cells
Meissner, A., Mikkelsen, T. S., Gu, H., Wernig, M., Hanna, J., Sivachenko, A., Zhang, X., Bernstein, B. E., Nusbaum, C., Jaffe, D. B., et al. (2008) · Nature · doi:10.1038/nature07107 · Cited by 2,536 · see citing works
Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors
Okita, K., Nakagawa, M., Hong, H., Ichisaka, T., & Yamanaka, S. (2008) · Science · doi:10.1126/science.1164270 · Cited by 2,002 · see citing works
Murry, C., & Keller, G. (2008) · Cell · doi:10.1016/j.cell.2008.02.008 · Cited by 1,805 · see citing works
Maherali, N., Sridharan, R., Xie, W., Utikal, J., Eminli, S., Arnold, K., Stadtfeld, M., Yachechko, R., Tchieu, J., Jaenisch, R., et al. (2007) · Cell stem cell · doi:10.1016/j.stem.2007.05.014 · Cited by 1,725 · see citing works
Self-citationInduced pluripotent stem cells generated without viral integration
Stadtfeld, M., Nagaya, M., Utikal, J., Weir, G., & Hochedlinger, K. (2008) · Science · doi:10.1126/science.1162494 · Cited by 1,683 · see citing works
Self-citationTreatment of Sickle Cell Anemia Mouse Model with iPS Cells Generated from Autologous Skin
Hanna, J. H., Wernig, M., Markoulaki, S., Sun, C., Meissner, A., Cassady, J. P., Beard, C., Brambrink, T., Wu, L.-C., Townes, T. M., et al. (2007) · Science · doi:10.1126/science.1152092 · Cited by 1,493 · see citing works
Dissecting direct reprogramming through integrative genomic analysis
Mikkelsen, T. S., Hanna, J. H., Zhang, X., Ku, M., Wernig, M., Schorderet, P., Bernstein, B., Jaenisch, R., Lander, E. S., & Meissner, A. (2008) · Nature · doi:10.1038/nature07056 · Cited by 1,472 · see citing works
Wernig, M., Zhao, J.-P., Pruszak, J., Hedlund, E., Fu, D., Soldner, F., Broccoli, V., Constantine‐Paton, M., Ole Isacson, A., & Jaenisch, R. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0801677105 · Cited by 1,205 · see citing works
Szymczak-Workman, A. L., Workman, C. J., Wang, Y., Vignali, K. M., Dilioglou, S., Vanin, E. F., & Vignali, D. A. A. (2004) · Nature Biotechnology · doi:10.1038/nbt957 · Cited by 1,160 · see citing works
Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells.
Brambrink, T., Foreman, R. K., Welstead, G., Lengner, C., Wernig, M., Suh, H., & Jaenisch, R. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2008.01.004 · Cited by 892 · see citing works
Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse
Stadtfeld, M., Maherali, N., Breault, D. T., & Hochedlinger, K. (2008) · Cell stem cell · doi:10.1016/j.stem.2008.02.001 · Cited by 823 · see citing works
Self-citationA drug-inducible transgenic system for direct reprogramming of multiple somatic cell types
Wernig, M., Lengner, C. J., Hanna, J. H., Lodato, M. A., Steine, E. J., Foreman, R. K., Staerk, J., Markoulaki, S., & Jaenisch, R. (2008) · Nature Biotechnology · doi:10.1038/nbt1483 · Cited by 437 · see citing works
Small Increases in the Level of Sox2 Trigger the Differentiation of Mouse Embryonic Stem Cells
Kopp, J. L., Ormsbee, B. D., Desler, M. M., & Rizzino, A. (2008) · Stem Cells · doi:10.1634/stemcells.2007-0951 · Cited by 338 · see citing works
Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection
Blelloch, R. H., Venere, M., Yen, J. S., & Ramalho‐Santos, M. (2007) · Cell stem cell · doi:10.1016/j.stem.2007.08.008 · Cited by 278 · see citing works
Complete correction of murine Artemis immunodeficiency by lentiviral vector-mediated gene transfer
Mostoslavsky, G., Fabian, A. J., Rooney, S., Alt, F. W., & Mulligan, R. C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0608130103 · Cited by 157 · see citing works
Self-citationChinnasamy, D., Milsom, M. D., Shaffer, J., Neuenfeldt, J., Shaaban, A. F., Margison, G. P., Fairbairn, L., & Chinnasamy, N. (2006) · Virology Journal · doi:10.1186/1743-422x-3-14 · Cited by 81 · see citing works
Dual-promoter lentiviral system allows inducible expression of noxious proteins in macrophages
Pan, H., Mostoslavsky, G., Eruslanov, E. B., Kotton, D. N., & Kramnik, I. (2007) · Journal of Immunological Methods · doi:10.1016/j.jim.2007.09.009 · Cited by 41 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Virus-free induction of pluripotency and subsequent excision of reprogramming factors
Kaji, K., Norrby, K., Paca, A., Mileikovsky, M., Mohseni, P., & Woltjen, K. (2009) · Nature · doi:10.1038/nature07864 · Cited by 1,252 · see citing works
Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency
Anokye‐Danso, F., Trivedi, C. M., Juhr, D., Gupta, M., Cui, Z., Tian, Y., Zhang, Y., Yang, W., Gruber, P. J., Epstein, J. A., et al. (2011) · Cell stem cell · doi:10.1016/j.stem.2011.03.001 · Cited by 1,206 · see citing works
The promise of induced pluripotent stem cells in research and therapy
Robinton, D., & Daley, G. (2012) · Nature · doi:10.1038/nature10761 · Cited by 1,152 · see citing works
Zhang, G., & Tandon, A. (2012) · Scientific Reports · doi:10.1038/srep00415 · Cited by 1,039 · see citing works
A Molecular Roadmap of Reprogramming Somatic Cells into iPS Cells
Polo, J. M., Anderssen, E., Walsh, R. M., Schwarz, B. A., Nefzger, C. M., Lim, S. M., Borkent, M., Apostolou, E., Alaei, S., Cloutier, J., et al. (2012) · Cell · doi:10.1016/j.cell.2012.11.039 · Cited by 861 · see citing works
Self-citationImmortalization eliminates a roadblock during cellular reprogramming into iPS cells
Utikal, J. S., Polo, J. M., Stadtfeld, M., Maherali, N., Kulalert, W., Walsh, R. M., Khalil, A., Rheinwald, J. G., & Hochedlinger, K. (2009) · Nature · doi:10.1038/nature08285 · Cited by 828 · see citing works
Self-citationInduced pluripotency: history, mechanisms, and applications.Preprint
Stadtfeld, M., & Hochedlinger, K. (2010) · Genes & Development · doi:10.1101/gad.1963910 · Cited by 851 · see citing works
Self-citationHuman iPSC-based modeling of late-onset disease via progerin-induced aging
Miller, J. D., Ganat, Y. M., Kishinevsky, S., Bowman, R. L., Liu, B., Tu, E. Y., Mandal, P. K., Vera, E., Shim, J.-W., Kriks, S., et al. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.11.006 · Cited by 777 · see citing works
Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells
Stadtfeld, M., Apostolou, E., Akutsu, H., Fukuda, A., Follett, P., Natesan, S., Kono, T., Shioda, T., & Hochedlinger, K. (2010) · Nature · doi:10.1038/nature09017 · Cited by 763 · see citing works
Self-citationBan, H., Nishishita, N., Fusaki, N., Tabata, T., Saeki, K., Shikamura, M., Takada, N., Inoue, M., Hasegawa, M., Kawamata, S., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1103509108 · Cited by 604 · see citing works
Direct reprogramming of mouse fibroblasts to neural progenitors
Kim, J., Efe, J. A., Zhu, S., Talantova, M. V., Xu, Y., Wang, S., Lipton, S. A., Zhang, K., & Ding, S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1103113108 · Cited by 568 · see citing works
Adenoviral gene delivery can reprogram human fibroblasts to induced pluripotent stem cells
Zhou, W., & Freed, C. R. (2009) · Stem Cells · doi:10.1002/stem.201 · Cited by 538 · see citing works
Deterministic direct reprogramming of somatic cells to pluripotency
Rais, Y., Zviran, A., Geula, S., Gafni, O., Chomsky, E., Viukov, S., Mansour, A. A., Caspi, I., Krupalnik, V., Zerbib, M., et al. (2013) · Nature · doi:10.1038/nature12587 · Cited by 522 · see citing works
Methods for making induced pluripotent stem cells: reprogramming à la carte
Gonzãlez, F., Boué, S., & Belmonte, J. C. I. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2937 · Cited by 504 · see citing works
SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients
Shcheglovitov, A., Shcheglovitova, O., Yazawa, M., Portmann, T., Shu, R., Sebastiano, V., Krawisz, A., Froehlich, W., Bernstein, J. A., Hallmayer, J. F., et al. (2013) · Nature · doi:10.1038/nature12618 · Cited by 470 · see citing works
Eminli, S., Foudi, A., Stadtfeld, M., Maherali, N., Ahfeldt, T., Mostoslavsky, G., Hock, H. R., & Hochedlinger, K. (2009) · Nature Genetics · doi:10.1038/ng.428 · Cited by 438 · see citing works
Self-citationNANOG-dependent function of TET1 and TET2 in establishment of pluripotency
Costa, Y., Ding, J., Theunissen, T. W., Faiola, F., Hore, T. A., Shliaha, P. V., Fidalgo, M., Saunders, A., Lawrence, M., Dietmann, S., et al. (2013) · Nature · doi:10.1038/nature11925 · Cited by 433 · see citing works
Somers, A., Jean, J.-C., Sommer, C. A., Omari, A., Ford, C. C., Mills, J. A., Ying, L., Sommer, A. G., Jean, J. M., Smith, B. W., et al. (2010) · Stem Cells · doi:10.1002/stem.495 · Cited by 423 · see citing works
Self-citationGuha, P., Morgan, J. W., Mostoslavsky, G., Rodrigues, N. P., & Boyd, A. S. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.01.006 · Cited by 368 · see citing works
Self-citationA Review of the Methods for Human iPSC Derivation
Malik, N., & Rao, M. S. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-348-0_3 · Cited by 355 · see citing works
Deconstructing transcriptional heterogeneity in pluripotent stem cells
Kumar, R. M., Cahan, P., Shalek, A. K., Satija, R., Jay DaleyKeyser, A., Li, H., Zhang, J., Pardee, K., Gennert, D., Trombetta, J. J., et al. (2014) · Nature · doi:10.1038/nature13920 · Cited by 356 · see citing works
Tgfβ Signal Inhibition Cooperates in the Induction of iPSCs and Replaces Sox2 and cMyc
Maherali, N., & Hochedlinger, K. (2009) · Current Biology · doi:10.1016/j.cub.2009.08.025 · Cited by 348 · see citing works
Self-citationRecent Advances in Lentiviral Vector Development and Applications
Mátrai, J., Chuah, M., & VandenDriessche, T. (2010) · Molecular Therapy · doi:10.1038/mt.2009.319 · Cited by 349 · see citing works
Progress toward the clinical application of patient-specific pluripotent stem cells
Kiskinis, E., & Eggan, K. (2010) · Journal of Clinical Investigation · doi:10.1172/jci40553 · Cited by 339 · see citing works
Wakao, S., Kitada, M., Kuroda, Y., Shigemoto, T., Matsuse, D., Akashi, H., Tanimura, Y., Tsuchiyama, K., Kikuchi, T., Goda, M., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1100816108 · Cited by 315 · see citing works
Tucker, B. A., Park, I., Qi, S. D., Klassen, H. J., Jiang, C., Yao, J., Redenti, S., Daley, G. Q., & Young, M. J. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018992 · Cited by 311 · see citing works
Sebastiano, V., Maeder, M. L., Angstman, J. F., Haddad, B., Khayter, C., Yeo, D. T., Goodwin, M. J., Hawkins, J. S., Ramirez, C. L., Batista, L. F. Z., et al. (2011) · Stem Cells · doi:10.1002/stem.718 · Cited by 307 · see citing works
Stem Cells and Cell Therapies in Lung Biology and Lung Diseases
Weiss, D. J., Kolls, J. K., Ortiz, L. A., Panoskaltsis‐Mortari, A., & Prockop, D. J. (2008) · Proceedings of the American Thoracic Society · doi:10.1513/pats.200804-037dw · Cited by 308 · see citing works
Progress in understanding reprogramming to the induced pluripotent state
Plath, K., & Lowry, W. E. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2955 · Cited by 285 · see citing works
Papapetrou, E. P., Tomishima, M. J., Chambers, S. M., Mica, Y., Reed, E., Menon, J., Tabar, V., Mo, Q., Studer, L., & Sadelain, M. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0904825106 · Cited by 286 · see citing works
Stadtfeld, M., Apostolou, E., Ferrari, F., Choi, J., Walsh, R. M., Chen, T., Ooi, S. K. T., Kim, S. Y., Bestor, T. H., Shioda, T., et al. (2012) · Nature Genetics · doi:10.1038/ng.1110 · Cited by 281 · see citing works
Self-citationFemale human iPS cells retain an inactive X-chromosome
Tchieu, J., Kuoy, E., Chin, M. H., Trinh, H., Patterson, M., Sherman, S. P., Aimiuwu, O., Lindgren, A. G., Hakimian, S., Zack, J., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.06.024 · Cited by 294 · see citing works
Wörsdörfer, P., Dalda, N., Kern, A., Krüger, S., Wagner, N., Kwok, C. K., Henke, E., & Ergün, S. (2019) · Scientific Reports · doi:10.1038/s41598-019-52204-7 · Cited by 276 · see citing works
Sommer, C., Sommer, A. G., Longmire, T. A., Christodoulou, C., Thomas, D. D., Gostissa, M., Alt, F. W., Murphy, G. J., Kotton, D. N., & Mostoslavsky, G. (2009) · Stem Cells · doi:10.1002/stem.255 · Cited by 269 · see citing works
Self-citationBatista, L. F. Z., Pech, M. F., Zhong, F. L., Nguyen, H. N., Xie, K. T., Zaug, A. J., Crary, S. M., Choi, J., Sebastiano, V., Cherry, A. M., et al. (2011) · Nature · doi:10.1038/nature10084 · Cited by 260 · see citing works
Ehrlich, M., Mozafari, S., Glatza, M., Starost, L., Velychko, S., Hallmann, A.-L., Cui, Q.-L., Schambach, A., Kim, K.-P., Bachelin, C., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1614412114 · Cited by 257 · see citing works
Yakubov, E., Rechavi, G., Rozenblatt, S., & Givol, D. (2010) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2010.02.150 · Cited by 240 · see citing works
Liang, Q., Fujita, R., Yamashita, T., Angulo, S. L., Rhinn, H., Rhee, D. B., Doege, C. A., Chau, L., Aubry, L., Vanti, W. B., et al. (2011) · Cell · doi:10.1016/j.cell.2011.07.007 · Cited by 230 · see citing works
RetractedInduced Pluripotent Stem Cells: Problems and Advantages when Applying them in Regenerative Medicine
Medvedev, S., Shevchenko, A. I., & Zakian, S. (2010) · Acta Naturae · doi:10.32607/20758251-2010-2-2-18-27 · Cited by 220 · see citing works
Buecker, C., Chen, H.-H., Polo, J. M., Daheron, L. M., Bu, L., Barakat, T. S., Okwieka, P., Porter, A. C. G., Gribnau, J. H., Hochedlinger, K., et al. (2010) · Cell stem cell · doi:10.1016/j.stem.2010.05.003 · Cited by 221 · see citing works
Self-citationChang, C.-W., Lai, Y.-S., Pawlik, K. M., Liu, K.-M., Sun, C.-W., Li, C., Schoeb, T., & Townes, T. (2009) · Stem Cells · doi:10.1002/stem.39 · Cited by 229 · see citing works
Ye, L., Chang, J. C., Lin, C., Sun, X.-F., Yu, J.-W., & Kan, Y. (2009) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.0904689106 · Cited by 211 · see citing works
Sebastiano, V., Zhen, H. H., Haddad, B., Bashkirova, E., Melo, S. P., Wang, P., Leung, T. L., Siprashvili, Z., Tichy, A., Li, J., et al. (2014) · Science Translational Medicine · doi:10.1126/scitranslmed.3009540 · Cited by 213 · see citing works
Application of Induced Pluripotent Stem (iPS) Cells in Periodontal Tissue Regeneration
Duan, X.-J., Tu, Q., Zhang, J., Ye, J.-H., Sommer, C., Mostoslavsky, G., Kaplan, D., Yang, P.-S., & Chen, J. (2011) · Journal of Cellular Physiology · doi:10.1002/jcp.22316 · Cited by 206 · see citing works
Self-citationA reprogrammable mouse strain from gene-targeted embryonic stem cells
Stadtfeld, M., Maherali, N., Borkent, M., & Hochedlinger, K. (2009) · Nature Methods · doi:10.1038/nmeth.1409 · Cited by 208 · see citing works
Self-citationOmole, A. E., & Fakoya, A. O. J. (2018) · PeerJ · doi:10.7717/peerj.4370 · Cited by 207 · see citing works
Stem Cell Therapy for Vascular Regeneration: Adult, Embryonic, and Induced Pluripotent Stem Cells
Leeper, N. J., Hunter, A. L., & Cooke, J. P. (2010) · Circulation · doi:10.1161/circulationaha.109.881441 · Cited by 195 · see citing works
Terrenoire, C., Wang, K., Tung, K. W. C., Chung, W. K., Pass, R. H., Lu, J. T., Jean, J., Omari, A., Sampson, K. J., Kotton, D. N., et al. (2012) · The Journal of General Physiology · doi:10.1085/jgp.201210899 · Cited by 195 · see citing works
Self-citationStreckfuss-Bömeke, K., Wolf, F., Azizian, A., Stauske, M., Tiburcy, M., Wagner, S., Hübscher, D., Dressel, R., Chen, S., Jende, J., et al. (2013) · European Heart Journal · doi:10.1093/eurheartj/ehs203 · Cited by 175 · see citing works
Wang, L., Du, Y., Ward, J. M., Shimbo, T., Lackford, B. L., Zheng, X., Miao, Y., Zhou, B., Han, L., Fargo, D. C., et al. (2014) · Cell stem cell · doi:10.1016/j.stem.2014.02.013 · Cited by 175 · see citing works
Novak, A., Barad, L., Zeevi‐Levin, N., Shick, R., Shtrichman, R., Lorber, A., Itskovitz‐Eldor, J., & Binah, O. (2011) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2011.01476.x · Cited by 178 · see citing works
DNA Damage Responses in Human Induced Pluripotent Stem Cells and Embryonic Stem Cells
Momčilović, O., Knobloch, L., Fornsaglio, J. L., Varum, S., Easley, C. A., & Schatten, G. P. (2010) · PLoS ONE · doi:10.1371/journal.pone.0013410 · Cited by 174 · see citing works
MBD3/NuRD Facilitates Induction of Pluripotency in a Context-Dependent Manner
dos Santos, R. L., Tosti, L., Radzisheuskaya, A., Caballero, I. M., Kaji, K., Hendrich, B., & Silva, J. (2014) · Cell stem cell · doi:10.1016/j.stem.2014.04.019 · Cited by 172 · see citing works
Phage‐Enabled Nanomedicine: From Probes to Therapeutics in Precision Medicine
Sunderland, K. S., Yang, M., & Mao, C. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201606181 · Cited by 171 · see citing works
Stem Cells and Cell Therapies in Lung Biology and Lung Diseases
Weiss, D. J., Bertoncello, I., Borok, Z., Kim, C., Panoskaltsis‐Mortari, A., Reynolds, S., Rojas, M., Stripp, B. R., Warburton, D., & Prockop, D. J. (2011) · Proceedings of the American Thoracic Society · doi:10.1513/pats.201012-071dw · Cited by 170 · see citing works
A DNA Repair Complex Functions as an Oct4/Sox2 Coactivator in Embryonic Stem Cells
Fong, Y. W., Inouye, C. J., Yamaguchi, T., Cattoglio, C., Grubisic, I., & Tjian, R. (2011) · Cell · doi:10.1016/j.cell.2011.08.038 · Cited by 164 · see citing works
Molecular Analyses of Human Induced Pluripotent Stem Cells and Embryonic Stem Cells
Chin, M. H., Pellegrini, M. A., Plath, K., & Lowry, W. E. (2010) · Cell stem cell · doi:10.1016/j.stem.2010.06.019 · Cited by 160 · see citing works
Ye, J., Xu, Y., Gao, J., Yan, S., Zhao, J., Tu, Q., Zhang, J., Duan, X.-J., Sommer, C., Mostoslavsky, G., et al. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.03.053 · Cited by 158 · see citing works
Self-citationFeeder-Free Derivation of Human Induced Pluripotent Stem Cells with Messenger RNA
Warren, L., Ni, Y., Wang, J., & Guo, X. (2012) · Scientific Reports · doi:10.1038/srep00657 · Cited by 154 · see citing works
Etchegaray, J., Chávez, L., Huang, Y., Ross, K. N., Choi, J., Martínez-Pastor, B., Walsh, R. M., Sommer, C., Lienhard, M., Gladden, A. D., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3147 · Cited by 155 · see citing works
Self-citationSmith, A. S. T., Macadangdang, J., Leung, W., Laflamme, M., & Kim, D.-H. (2016) · Biotechnology Advances · doi:10.1016/j.biotechadv.2016.12.002 · Cited by 137 · see citing works
Cell-based transplantation strategies to promote plasticity following spinal cord injury
Ruff, C. A., Wilcox, J. T., & Fehlings, M. G. (2011) · Experimental Neurology · doi:10.1016/j.expneurol.2011.02.010 · Cited by 149 · see citing works
Denton, K. R., Lei, L., Grenier, J. M., Rodionov, V., Blackstone, C., & Li, X.-J. (2014) · Stem Cells · doi:10.1002/stem.1569 · Cited by 133 · see citing works
Genomic instability in induced stem cells
Pasi, C. E., Dereli-Oz, A., Negrini, S., Friedli, M., Fragola, G., Lombardo, A., Houwe, G. V., Naldini, L., Casola, S. S., Testa, G., et al. (2011) · Cell Death and Differentiation · doi:10.1038/cdd.2011.9 · Cited by 146 · see citing works
Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke
Wei, L., Wei, Z. Z., Jiang, M. Q., Mohamad, O., & Yu, S. P. (2017) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2017.03.003 · Cited by 143 · see citing works
Widespread mitotic bookmarking by histone marks and transcription factors in pluripotent stem cells
Liu, Y.-Y., Pelham-Webb, B., Giammartino, D. C. D., Li, J.-X., Kim, D., Kita, K., Saiz, N., Garg, V., Doane, A., Giannakakou, P., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.04.067 · Cited by 142 · see citing works
Induced Pluripotent Stem Cells: Reprogramming Platforms and Applications in Cell Replacement Therapy
Abbar, A. A., Ngai, S. C., Nograles, N., Alhaji, S. Y., & Abdullah, S. (2020) · BioResearch open access · doi:10.1089/biores.2019.0046 · Cited by 136 · see citing works
piggyBac-ing models and new therapeutic strategies
Woodard, L. E., & Wilson, M. H. (2015) · Trends in biotechnology · doi:10.1016/j.tibtech.2015.06.009 · Cited by 133 · see citing works
Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs
Velychko, S., Adachi, K., Kim, K.-P., Hou, Y., MacCarthy, C. M., Wu, G., & Schöler, H. R. (2019) · Cell stem cell · doi:10.1016/j.stem.2019.10.002 · Cited by 131 · see citing works
Motion of VAPB molecules reveals ER–mitochondria contact site subdomains
Obara, C. J., Nixon‐Abell, J., Moore, A. S., Riccio, F., Hoffman, D. P., Shtengel, G. E., Xu, C. S., Schaefer, K., Pasolli, H. A., Masson, J., et al. (2024) · Nature · doi:10.1038/s41586-023-06956-y · Cited by 130 · see citing works
Preconditioning and Stem Cell Survival
Haider, H. K., & Ashraf, M. (2009) · Journal of Cardiovascular Translational Research · doi:10.1007/s12265-009-9161-2 · Cited by 129 · see citing works
Pamies, D. (2018) · ALTEX · doi:10.14573/altex.1710081 · Cited by 127 · see citing works
Smg6/Est1 licenses embryonic stem cell differentiation via nonsense‐mediated mRNA decay
Li, T., Shi, Y. X., Wang, P., Guachalla, L. M., Sun, B., Jörß, T., Chen, Y., Groth, M., Krueger, A., Platzer, M., et al. (2015) · The EMBO Journal · doi:10.15252/embj.201489947 · Cited by 121 · see citing works
Assessing iPSC reprogramming methods for their suitability in translational medicine
Rao, M. S., & Malik, N. (2012) · Journal of Cellular Biochemistry · doi:10.1002/jcb.24183 · Cited by 126 · see citing works
Chou, S. T., Byrska‐Bishop, M. S., Tober, J. M., Yao, Y., VanDorn, D., Opalinska, J., Mills, J. A., Choi, J. K., Speck, N. A., Gadue, P. J., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1211175109 · Cited by 125 · see citing works
Global epigenetic changes during somatic cell reprogramming to iPS cells
Mattout, A., Biran, A., & Meshorer, E. (2011) · Journal of Molecular Cell Biology · doi:10.1093/jmcb/mjr028 · Cited by 124 · see citing works
Pelham‐Webb, B., Polyzos, A., Wojenski, L., Kloetgen, A., Li, J., Giammartino, D. C. D., Sakellaropoulos, T., Tsirigos, A., Core, L. J., & Apostolou, E. (2021) · Molecular Cell · doi:10.1016/j.molcel.2021.02.032 · Cited by 118 · see citing works
Pawlak, M., & Jaenisch, R. (2011) · Genes & Development · doi:10.1101/gad.2039011 · Cited by 119 · see citing works
New developments in lentiviral vector design, production and purification
Segura, M. M., Mangion, M., Gaillet, B., & Garnier, A. (2013) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2013.779249 · Cited by 126 · see citing works
Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity
Wu, C., & Dunbar, C. E. (2011) · Frontiers of Medicine · doi:10.1007/s11684-011-0159-1 · Cited by 114 · see citing works
ALS-Linked Mutations Enlarge TDP-43-Enriched Neuronal RNA Granules in the Dendritic Arbor
Liu‐Yesucevitz, L., Lin, A. Y., Ebata, A., Boon, J. Y., Reid, W., Xu, Y.-F., Kobrin, K. L., Murphy, G. J., Petrucelli, L., & Wolozin, B. L. (2014) · Journal of Neuroscience · doi:10.1523/jneurosci.2350-13.2014 · Cited by 113 · see citing works
Self-citationInduced pluripotent stem cells: a new revolution for clinical neurology?
Mattis, V. B., & Svendsen, C. N. (2011) · The Lancet Neurology · doi:10.1016/s1474-4422(11)70022-9 · Cited by 112 · see citing works
Salvarani, N., Crasto, S., Miragoli, M., Bertero, A., Paulis, M., Kunderfranco, P., Serio, S., Forni, A., Lucarelli, C., Ferro, M. D., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-09929-w · Cited by 113 · see citing works
Wang, Y., Tian, Y., Morley, M. P., Lu, M. M., DeMayo, F. J., Olson, E. N., & Morrisey, E. E. (2013) · Developmental Cell · doi:10.1016/j.devcel.2013.01.012 · Cited by 111 · see citing works
Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage
Bar‐Nur, O., Verheul, C., Sommer, A. G., Brumbaugh, J., Schwarz, B. A., Lipchina, I., Huebner, A. J., Mostoslavsky, G., & Hochedlinger, K. (2015) · Nature Biotechnology · doi:10.1038/nbt.3247 · Cited by 109 · see citing works
Self-citationTsix RNA and the Germline Factor, PRDM14, Link X Reactivation and Stem Cell Reprogramming
Payer, B., Rosenberg, M. A., Yamaji, M., Yabuta, Y., Koyanagi‐Aoi, M., Hayashi, K., Yamanaka, S., Saitou, M., & Lee, J. T. (2013) · Molecular Cell · doi:10.1016/j.molcel.2013.10.023 · Cited by 109 · see citing works
All Roads Lead to Induced Pluripotent Stem Cells: The Technologies of iPSC Generation
Hu, K. (2014) · Stem Cells and Development · doi:10.1089/scd.2013.0620 · Cited by 105 · see citing works
Functional Consequences of Human Induced Pluripotent Stem Cell Therapy
Xiong, Q., Ye, L., Zhang, P., Lepley, M. A., Tian, J., Li, J., Zhang, L., Swingen, C., Vaughan, J. T., Kaufman, D. S., et al. (2013) · Circulation · doi:10.1161/circulationaha.112.000641 · Cited by 104 · see citing works
Disrupted neuronal maturation in Angelman syndrome-derived induced pluripotent stem cells
Fink, J. J., Robinson, T. M., Germain, N. D., Sirois, C. L., Bolduc, K. A., Ward, A. J., Rigo, F., Chamberlain, S. J., & Levine, E. S. (2017) · Nature Communications · doi:10.1038/ncomms15038 · Cited by 102 · see citing works
Dectin-1–Dependent LC3 Recruitment to Phagosomes Enhances Fungicidal Activity in Macrophages
Tam, J. M., Mansour, M. K., Khan, N. S., Seward, M., Puranam, S., Tanne, A., Sokolovska, A., Becker, C. E., Acharya, M., Baird, M. A., et al. (2014) · The Journal of Infectious Diseases · doi:10.1093/infdis/jiu290 · Cited by 101 · see citing works
Germain, N. D., Chen, P.-F., Plocik, A. M., Glatt-Deeley, H. R., Brown, J. D., Fink, J. J., Bolduc, K. A., Robinson, T. M., Levine, E. S., Reiter, L. T., et al. (2014) · Molecular Autism · doi:10.1186/2040-2392-5-44 · Cited by 101 · see citing works
Meng, X., Neises, A., Su, R. J., Payne, K. J., Ritter, L. M., Gridley, D. S., Wang, J., Sheng, M. H. ‐., Lau, K. ‐. W., Baylink, D. J., et al. (2011) · Molecular Therapy · doi:10.1038/mt.2011.258 · Cited by 97 · see citing works
Lentiviral Vectors: Their Molecular Design, Safety, and Use in Laboratory and Preclinical Research
Dropulić, B. (2011) · Human Gene Therapy · doi:10.1089/hum.2011.058 · Cited by 97 · see citing works
Su, R. J., Baylink, D. J., Neises, A., Kiroyan, J. B., Meng, X., Payne, K. J., Tschudy-Seney, B., Duan, Y., Appleby, N. C., Kearns‐Jonker, M., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0064496 · Cited by 96 · see citing works
Demestre, M., Orth, M., Föhr, K. J., Achberger, K., Ludolph, A. C., Liebau, S., & Boeckers, T. M. (2015) · Stem Cell Research · doi:10.1016/j.scr.2015.07.005 · Cited by 95 · see citing works
AID stabilizes stem-cell phenotype by removing epigenetic memory of pluripotency genes
Kumar, R., DiMenna, L. J., Schrode, N., Liu, T.-C., Franck, P., Descalzo, S. M., Hadjantonakis, A., Zarrin, A. A., Chaudhuri, J., Elemento, O., et al. (2013) · Nature · doi:10.1038/nature12299 · Cited by 94 · see citing works
Ho, R., Papp, B., Hoffman, J. A., Merrill, B. J., & Plath, K. (2013) · Cell Reports · doi:10.1016/j.celrep.2013.05.015 · Cited by 92 · see citing works
Pakzad, M., Totonchi, M., Taei, A., Seifinejad, A., Hassani, S., & Baharvand, H. (2009) · Stem Cell Reviews and Reports · doi:10.1007/s12015-009-9103-z · Cited by 92 · see citing works
Panicker, L. M., Miller, D., Awad, O., Bose, V., Lun, Y., Park, T. S., Zambidis, E. T., Sgambato, J. A., & Feldman, R. A. (2014) · Stem Cells · doi:10.1002/stem.1732 · Cited by 92 · see citing works
The role of DNA repair in the pluripotency and differentiation of human stem cells
Rocha, C. R. R., Lerner, L. K., Okamoto, O. K., Marchetto, M. C., & Menck, C. F. M. (2012) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2012.09.001 · Cited by 93 · see citing works
- Authors
- Sommer, C. A., Stadtfeld, M., Murphy, G. J., Hochedlinger, K., Kotton, D. N., & Mostoslavsky, G.
- Affiliations
- Boston University (US)
- Massachusetts General Hospital (US)
- Harvard University (US)
- Corresponding author
- Mostoslavsky, Gustavo
- ORCID
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-03-01
- Volume
- 27
- Issue
- 3
- Pages
- 543-549
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- PMC
- PMC4848035
- OpenAlex
- W2038815755
- Cited by
- 676
- References
- 24
- Citations by year
- 2026: 6 · 2025: 13 · 2024: 16 · 2023: 15 · 2022: 18 · 2021: 23 · 2020: 30 · 2019: 35 · 2018: 42 · 2017: 41 · 2016: 41 · 2015: 49 · 2014: 61 · 2013: 65 · 2012: 66
- FWCI
- 21.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Stem Cells (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- V Foundation for Cancer Research
- National Institutes of Health (1dp2od003266-01)
- Harvard Stem Cell Institute
- National Heart, Lung, and Blood Institute (P01HL047049)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R21HD060308)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- induced pluripotent stem cells, lentiviral vectors, Oct4, KLF4, SOX2, c-Myc, 2A peptide, internal ribosome entry site, fibroblast reprogramming, chromosomal integration, teratoma assay, mouse chimeras, pluripotency
- MeSH terms
- Kruppel-Like Factor 4, Animals, Cells, Cultured, Genetic Vectors, Blotting, Southern, Lentivirus, Proto-Oncogene Proteins c-myc, Reverse Transcriptase Polymerase Chain Reaction, Pluripotent Stem Cells, Octamer Transcription Factor-3, Mice, Kruppel-Like Transcription Factors, SOXB1 Transcription Factors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
