Donor age and long-term culture affect differentiation and proliferation of human bone marrow mesenchymal stem cells
Abstract
Bone marrow-derived human mesenchymal stem cells (BM-hMSCs) represent a promising cell-based therapy for a number of degenerative conditions. Many applications require cell expansion and involve the treatment of diseases and conditions found in an aging population. Therefore, the effects of donor age and long-term passage must be clarified. In this study, the effects of donor age and long-term passage on the morphology, proliferation potential, characteristics, mesodermal differentiation ability, and transdifferentiation potential of hMSCs towards neurogenic lineage were evaluated. Cells from child donors (0-12 years, n = 6) maintained their fibroblast-like morphology up to higher passages and proliferated in a greater number than those from adult (25-50 years, n = 6) and old (over 60 years, n = 6) donors. Adipogenic, osteogenic, and neurogenic differentiation potential decreased with age, while chondrogenic potential did not change. Long-term passage affected the morphology and proliferation of hMSCs from all ages. With increasing passage number, proliferation rate decreased and cells lost their typical morphology. Expression levels of neural markers (β III tubulin and NSE) and topo II isoforms in populations of nondifferentiated hMSCs were investigated by reverse transcription polymerase chain reaction analysis. While neural marker and topo IIβ expression levels increased due to increasing passage number in adult hMSCs compared to child hMSCs, topo IIα decreased in both. These results indicated that, even under highly standardized culture conditions, donor age and long-term passage have effects on hMSC characteristics, which should be taken into account prior to stem cell-based therapies.
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Older donors of BM-MSCs have lower osteogenic, adipogenic and neurogenic capacities.
“with older donors of BM-MSCs having lower proliferation rates [246], [249] and osteogenic [246], [248], adipogenic and neurogenic [248] capacities.”
From Mesenchymal stem cell secretome for regenerative medicine: Where do we stand? (2025) · cites this paper as [248]
Donor age and sex can affect MSC activity and thus the properties of MSC-secretome.
“In addition, the number of MSC passage [246], [247], [248] and the donor variability [246], [247], [248] cannot be ignored since MSC activity and, thus, the properties of MSC-secretome can be affected by donor age and sex [246], [248], [249]”
From Mesenchymal stem cell secretome for regenerative medicine: Where do we stand? (2025) · cites this paper as [248]
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- Authors
- Zaim, M., Karaman, S., Cetin, G., & Isik, S.
- Affiliations
- Fatih University (TR)
- Şişli Etfal Eğitim ve Araştırma Hastanesi (TR)
- Venue
- Annals of Hematology
- Published
- 2012-03-08
- Volume
- 91
- Issue
- 8
- Pages
- 1175-1186
- ISSN
- 0939-5555 (ISSN-L)
- 1432-0584
- OpenAlex
- W1978468353
- Cited by
- 294
- References
- 41
- Citations by year
- 2026: 8 · 2025: 15 · 2024: 15 · 2023: 24 · 2022: 19 · 2021: 35 · 2020: 27 · 2019: 23 · 2018: 19 · 2017: 28 · 2016: 23 · 2015: 18 · 2014: 24 · 2013: 12 · 2012: 4
- FWCI
- 8.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Annals of Hematology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- mesenchymal stem cells, bone marrow, donor age, cellular aging, cell proliferation, multilineage differentiation, neurogenic differentiation, transdifferentiation, topoisomerase II, neural markers, stem cell therapy
- MeSH terms
- Adult, Age Factors, Aged, Aging, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Child, Child, Preschool, Female, Humans, Male, Middle Aged, Time Factors, Tissue Donors, Cell Proliferation, Mesenchymal Stem Cells, Primary Cell Culture
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