Cited by 15Ginkgo biloba and Cashew ApplicationsCholinesterase and Neurodegenerative DiseasesMedicinal Plants and Neuroprotection

Biochemical Constituent of Ginkgo biloba (Seed) 80% Methanol Extract Inhibits Cholinesterase Enzymes in Javanese Medaka (Oryzias javanicus) Model

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Hassan, Ibrahim, Wan Ibrahim, Wan Norhamidah, Yusuf, Ferdaus Mohamat, Ahmad, Siti Aqlima, Ahmad, Syahida·2020·Journal of Toxicology

Abstract

Background. Pathophysiological changes leading to the death of nerve cells present in the brain and spinal cord are referred to as neurodegenerative diseases. Presently, treatment of these diseases is not effective and encounters many challenges due to the cost of drug and side effects. Thus, the search for the alternative agents to replace synthetic drugs is in high demand. Therefore, the aim of this study is to evaluate the anticholinesterase properties of Ginkgo biloba seed. Methods. The seed was extracted with 80% methanol. Toxicity studies and evaluation of anticholinesterase activities were carried out in adult Javanese medaka (Oryzias javanicus). Phytochemical study to identify the bioactive lead constituents of the crude extract was also carried out using high performance liquid chromatography (HPLC). Results. The result shows activities with high significant differences at P<0.001 between the treated and nontreated groups. A bioactive compound (vitaxin) was identified with the aid of HPLC method. Conclusion. The presence of bioactive compound vitaxin is among the major secondary metabolites that contribute to increasing activities of this plant extract. High anticholinesterase activities and low toxicity effect of this plant show its benefit to be used as natural medicine or supplements.

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  1. [13]Ngamniyom A. Sriyapai T. Sriyapai P. Panyarachun B. Introduction of encysted metacercarial Stephanostomum sp. in Javanese ricefish (Oryzias javanicus) and bacterial diversity of encysts from mangrove swamps of Trang Province, Thailand Songklanakarin Journal of Science & Technology 2020 42 1

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Authors
Hassan, I., Wan Ibrahim, W. N., Yusuf, F. M., Ahmad, S. A., & Ahmad, S.
Affiliations
  • Universiti Putra Malaysia (MY)
  • Department of Biology, Faculty of Science, Universiti Putra Malaysia, UPM Serdang, Seri Kembangan 43400, Selangor, Malaysia
  • Department of Environmental Science, Faculty of Environmental Study, Universiti Putra Malaysia, UPM Serdang, Seri Kembangan 43400, Selangor, Malaysia
Corresponding author
Ahmad, Syahida
Venue
Journal of Toxicology
Publisher
Wiley
Published
2020-09-23
Volume
2020
Pages
1-11
ISSN
  • 1687-8191 (ISSN-L)
  • 1687-8205
OpenAlex
W3088687834
Cited by
15
References
45
Citations by year
2026: 3 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 1
FWCI
2.49
Type
journal article
Language
en
Open access
Locations
  • Journal of Toxicology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Greater South Information System
Funders
Ministry of Higher Education, Malaysia (TD-FRGS/2/2013/UPM/02/1/2)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Ginkgo biloba and Cashew Applications
  • Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
Keywords
Ginkgo biloba seeds, anticholinesterase activity, cholinesterase inhibition, neurodegenerative diseases, plant extract toxicity, phytochemical constituents, secondary metabolites, natural medicine

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