The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Alterations of Choline Phospholipid Metabolism in Ovarian Tumor Progression
Abstract
Recent characterization of abnormal phosphatidylcholine metabolism in tumor cells by nuclear magnetic resonance (NMR) has identified novel fingerprints of tumor progression that are potentially useful as clinical diagnostic indicators. In the present study, we analyzed the concentrations of phosphatidylcholine metabolites, activities of phosphocholine-producing enzymes, and uptake of [methyl-14C]choline in human epithelial ovarian carcinoma cell lines (EOC) compared with normal or immortalized ovary epithelial cells (EONT). Quantification of phosphatidylcholine metabolites contributing to the 1H NMR total choline resonance (3.20-3.24 ppm) revealed intracellular [phosphocholine] and [total choline] of 2.3 ± 0.9 and 5.2 ± 2.4 nmol/106 cells, respectively, with a glycerophosphocholine/phosphocholine ratio of 0.95 ± 0.93 in EONT cells; average [phosphocholine] was 3- to 8-fold higher in EOC cells (P < 0.0001), becoming the predominant phosphatidylcholine metabolite, whereas average glycerophosphocholine/phosphocholine values decreased significantly to ≤0.2. Two-dimensional {phosphocholine/total choline, [total choline]} and {glycerophosphocholine/total choline, [total choline]} maps allowed separate clustering of EOC from EONT cells (P < 0.0001, 95% confidence limits). Rates of choline kinase activity in EOC cells were 12- to 24-fold higher (P < 0.03) than those in EONT cells (basal rate, 0.5 ± 0.1 nmol/106 cells/h), accounting for a consistently elevated (5- to 15-fold) [methyl-14C]choline uptake after 1-hour incubation (P < 0.0001). The overall activity of phosphatidylcholine-specific phospholipase C and phospholipase D was also higher (∼5-fold) in EOC cells, suggesting that both biosynthetic and catabolic pathways of the phosphatidylcholine cycle likely contribute to phosphocholine accumulation. Evidence of abnormal phosphatidylcholine metabolism might have implications in EOC biology and might provide an avenue to the development of noninvasive clinical tools for EOC diagnosis and treatment follow-up.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Jemal, A., Tiwari, R. C., Murray, T., Ghafoor, A., Samuels, A., Ward, E., Feuer, E. J., & Thun, M. J. (2004) · CA: A Cancer Journal for Clinicians · doi:10.3322/canjclin.54.1.8 · Cited by 3,984
Rubin, M., & Stephen, C. D. (1993) · Cited by 3,149
Reactivity of a monoclonal antibody with human ovarian carcinoma.
Bast, R. C., Feeney, M., Lazarus, H., Nadler, L. M., Colvin, R. B., & Knapp, R. C. (1981) · Journal of Clinical Investigation · doi:10.1172/jci110380 · Cited by 1,661
One Hundred and Twenty-Seven Cultured Human Tumor Cell Lines Producing Tumors in Nude Mice23
Fogh, J., Fogh, J. M., & Orfeo, T. (1977) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/59.1.221 · Cited by 1,599
Studies of human tumors by MRS: A review
Negendank, W. (1992) · NMR in Biomedicine · doi:10.1002/nbm.1940050518 · Cited by 660
Tumour phospholipid metabolism
Podo, F. (1999) · NMR in Biomedicine · doi:10.1002/(sici)1099-1492(199911)12:7<413::aid-nbm587>3.0.co;2-u · Cited by 579
Self-citationAboagye, E. O., & Bhujwalla, Z. M. (1999) · PubMed · Cited by 546
Hamilton, T. C., Young, R. C., McKoy, W. M., Grotzinger, K. R., Green, J. A., Chu, E. W., Whang‐Peng, J., Rogan, A. M., Green, W. R., & Ozols, R. F. (1983) · PubMed · Cited by 539
Molecular Causes of the Aberrant Choline Phospholipid Metabolism in Breast Cancer
Glunde, K., Jie, C., & Bhujwalla, Z. M. (2004) · Cancer Research · doi:10.1158/0008-5472.can-03-3829 · Cited by 380
Phospholipid biosynthesis in mammalian cells
Vance, J. E., & Vance, D. E. (2004) · Biochemistry and Cell Biology · doi:10.1139/o03-073 · Cited by 373
Cited by
Serum metabolomics for early diagnosis of esophageal squamous cell carcinoma by UHPLC-QTOF/MS
Wang, J., Zhang, T., Shen, X., Liu, J., Zhao, D., Sun, Y., Wang, L., Liu, Y., Gong, X., Liu, Y., et al. (2016) · Metabolomics · doi:10.1007/s11306-016-1050-5 · Cited by 373
Pascucci, B., Lemma, T., Iorio, E., Giovannini, S., Vaz, B., Iavarone, I., Calcagnile, A., Narciso, L., Degan, P., Podo, F., et al. (2012) · Aging Cell · doi:10.1111/j.1474-9726.2012.00815.x · Cited by 104
Self-citationYip, K. W., Mao, X., Au, P. Y. B., Hedley, D. W., Chow, S., Dalili, S., Mocanu, J. D., Bastianutto, C., Schimmer, A., & Liu, F.-F. (2006) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-06-0536 · Cited by 99
Backshall, A., Alferez, D., Teichert, F., Wilson, I. D., Wilkinson, R. W., Goodlad, R. A., & Keun, H. C. (2009) · Journal of Proteome Research · doi:10.1021/pr800793w · Cited by 42
Cancer survival classification using integrated data sets and intermediate information
Kim, S., Park, T., & Kon, M. (2014) · Artificial Intelligence in Medicine · doi:10.1016/j.artmed.2014.06.003 · Cited by 25
Metadata
- Authors
- Iorio, E., Mezzanzanica, D., Alberti, P., Spadaro, F., Ramoni, C., D'Ascenzo, S., Millimaggi, D., Pavan, A., Dolo, V., Canevari, S., & Podo, F.
- Venue
- Cancer Research
- Published
- 2005-10-15
- Volume
- 65
- Issue
- 20
- Pages
- 9369-9376
- ISSN
- 0008-5472 (ISSN-L)
- 1538-7445
- OpenAlex
- W2154129829
- Cited by
- 309
- References
- 30
- FWCI
- 2.45
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Alterations of Choline Phospholipid Metabolism in Ovarian Tumor Progression. https://app.cochat.ai/papers/10.1158/0008-5472.can-05-1146
- The paper
- Iorio, E., Mezzanzanica, D., Alberti, P., Spadaro, F., Ramoni, C., D'Ascenzo, S., Millimaggi, D., Pavan, A., Dolo, V., Canevari, S., & Podo, F. (2005). Alterations of Choline Phospholipid Metabolism in Ovarian Tumor Progression. Cancer Research. https://doi.org/10.1158/0008-5472.can-05-1146
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
