Myeloid Cell–associated Resistance to PD-1/PD-L1 Blockade in Urothelial Cancer Revealed Through Bulk and Single-cell RNA Sequencing

Wang, Li; Sfakianos, John P.; Beaumont, Kristin G.; Akturk, Guray; Horowitz, Amir; Sebra, Robert P.; Farkas, Adam M.; Gnjatic, Sacha; Hake, Austin; Izadmehr, Sudeh; Wiklund, Peter; Oh, William K.; Szabo, Peter M.; Wind-Rotolo, Megan; Unsal-Kacmaz, Keziban; Yao, Xin; Schadt, Eric; Sharma, Padmanee; Bhardwaj, Nina; Zhu, Jun; Galsky, Matthew D.

doi:10.1158/1078-0432.ccr-20-4574

Myeloid Cell–associated Resistance to PD-1/PD-L1 Blockade in Urothelial Cancer Revealed Through Bulk and Single-cell RNA Sequencing

Purpose: To define dominant molecular and cellular features associated with PD-1/PD-L1 blockade resistance in metastatic urothelial cancer. Experimental Design: We pursued an unbiased approach using bulk RNA sequencing data from two clinical trials to discover (IMvigor 210) and validate (CheckMat…