Corticotropin–releasing factor receptors in human pituitary gland: autoradiographic localization
Abstract
Corticotropin-releasing factor (CRF) receptors were localized in the human pituitary gland by an in vitro labeling light microscopic autoradiographic technique using an 125I-labeled analog of ovine CRF substituted with norleucine and tyrosine at amino acid residues 21 and 32, respectively. Specific binding sites for CRF were observed in the anterior lobe while no specific binding sites for CRF were present in the posterior pituitary lobe. The CRF binding sites in the anterior lobe occurred in clusters which predominated in the anteromedial portion of the lobe. This pattern of localization of CRF binding sites resembles previously reported distributions of corticotrophs in anterior pituitary. These data support the physiological role of endogenous CRF in regulating hormone secretion from the anterior lobe of the human pituitary.
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Both CRH and AVP act on their own receptors on corticotrope cells in the anterior pituitary to stimulate ACTH production and release, with different or even similar potencies.
“Namely, it has been indicated that both CRH and AVP act on their own receptors (28–32) on corticotrope cells in anterior pituitary to stimulate adrenocorticotropic hormone (ACTH) production and release with different (26, 33–41) or even similar potencies (42).”
From Influence of arginine vasopressin on the ultradian dynamics of Hypothalamic-Pituitary-Adrenal axis (2022) · cites this paper as [29]
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- Authors
- De Souza, E. B., Perrin, M. H., Whitehouse, P. J., Rivier, J., Vale, W., & Kuhar, M. J.
- Affiliations
- Salk Institute for Biological Studies (US)
- Johns Hopkins University (US)
- Venue
- Neuroendocrinology
- Publisher
- S. Karger AG
- Published
- 2008-03-28
- Volume
- 40
- Issue
- 5
- Pages
- 419-422
- ISSN
- 0028-3835 (ISSN-L)
- 1423-0194
- OpenAlex
- W2088988828
- Cited by
- 66
- References
- 0
- Citations by year
- 2022: 1 · 2014: 1
- FWCI
- 0.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroendocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (2p01am026741-06, 2k05mh000053-11, 5r01mh025951-13)
- National Institute of Mental Health (MH25951, MH 00053)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- corticotropin-releasing factor, CRF receptors, human pituitary, anterior pituitary, neurohypophysis, corticotrophs, hormone secretion regulation, receptor localization
- MeSH terms
- Autoradiography, Binding, Competitive, Corticotropin-Releasing Hormone, Humans, Peptide Fragments, Pituitary Gland, Pituitary Gland, Anterior, Receptors, Cell Surface, Tissue Distribution, Receptors, Corticotropin-Releasing Hormone
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