Collectin-11 Is an Important Modulator of Retinal Pigment Epithelial Cell Phagocytosis and Cytokine Production
Open accessAbstract
In this paper, we report previously unknown roles for collectin-11 (CL-11, a soluble C-type lectin) in modulating the retinal pigment epithelial (RPE) cell functions of phagocytosis and cytokine production. We found that CL-11 and its carbohydrate ligand are expressed in both the murine and human neural retina; these resemble each other in terms of RPE and photoreceptor cells. Functional analysis of murine RPE cells showed that CL-11 facilitates the opsonophagocytosis of photoreceptor outer segments and apoptotic cells, and also upregulates IL-10 production. Mechanistic analysis revealed that calreticulin on the RPE cells is required for CL-11-mediated opsonophagocytosis whereas signal-regulatory protein α and mannosyl residues on the cells are involved in the CL-11-mediated upregulation of IL-10 production. This study is the first to demonstrate the role of CL-11 and the molecular mechanisms involved in modulating RPE cell phagocytosis and cytokine production. It provides a new insight into retinal health and disease and has implications for other phagocytic cells.
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- Authors
- Dong, X., Wu, W., Ma, L., Liu, C., Bhuckory, M. B., Wang, L., Nandrot, E. F., Xu, H., Li, K., Liu, Y., & Zhou, W.
- Affiliations
- Sun Yat-sen University (CN)
- Guy's Hospital (GB)
- Second Affiliated Hospital of Xi'an Jiaotong University (CN)
- Queen's University Belfast (GB)
- Institut de la Vision (FR)
- Centre National de la Recherche Scientifique (FR)
- ORCID
- Venue
- Journal of Innate Immunity
- Publisher
- S. Karger AG
- Published
- 2017
- Volume
- 9
- Issue
- 6
- Pages
- 529-545
- ISSN
- 1662-811X (ISSN-L)
- 1662-8128
- PMC
- PMC6738776
- OpenAlex
- W2742249326
- Cited by
- 23
- References
- 33
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2023: 5 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 1
- FWCI
- 1.60
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Innate Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Research Portal (King's College London) (publishedVersion, other-oa)
- HAL (submittedVersion, other-oa)
- Research Portal (Queen's University Belfast) (submittedVersion)
- Newcastle University ePrints (Newcastle Univesity)
- Funders
- National Science Foundation
- National Natural Science Foundation of China
- Medical Research Council (MR/L020254/1, MR/J006742/1, MR/M012263/1)
- Topics
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Life Sciences › Immunology and Microbiology › Immunology › Biomarkers in Disease Mechanisms
- Keywords
- collectin-11, retinal pigment epithelium, phagocytosis, opsonophagocytosis, photoreceptor outer segments, efferocytosis, IL-10, calreticulin, SIRPα, mannosyl residues, C-type lectin, cytokine production, retinal health
- MeSH terms
- Animals, Cells, Cultured, Humans, Mice, Inbred C57BL, Retina, Retinal Pigments, Up-Regulation, Interleukin-10, Mice, Knockout, Collectins, Calreticulin, Mice, Retinal Pigment Epithelium, Cytophagocytosis
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