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Cited by 4Antibiotic Resistance in BacteriaAntibiotic Use and ResistancePharmaceutical and Antibiotic Environmental Impacts

Plasmid-mediated quinolone resistance determinants in clinical bacterial pathogens isolated from the Western Region of Ghana: a cross-sectional study

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Sah, Andrews Kwabena, Feglo, Patrick Kwame·2022·Pan African Medical Journal
DOI 10.11604/pamj.2022.43.207.33734PMC PMC10024564
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Abstract

Introduction: quinolones are critically important antibiotics that are reserved for treating very severe infections caused by multidrug-resistant bacterial pathogens. However, their indiscriminate uses have resulted in an increased number of resistant strains in many parts of the world including Ghana. We determined the quinolone resistance profile of Gram-negative bacterial pathogens and characterized the underlying molecular determinants of resistance. Methods: Gram-negative pathogens obtained from clinical specimens at three hospital laboratories were tested for resistance to quinolones and other commonly used antibiotics. ESBL production among the Enterobacterial isolates was confirmed using the combined disc diffusion method. We then used PCR to determine seven types of plasmid-mediated quinolone resistance genes present in the isolates resistant to nalidixic acid and ciprofloxacin. Results: in this study, 29.5% of the isolates were resistant to ciprofloxacin, with the highest of 50% among E. coli resistance to the other quinolones was levofloxacin (24.4%), norfloxacin (24.9%), and nalidixic acid (38.9%). Significant proportions of the quinolone-resistant isolates were ESBL producers (P-values < 0.001). The aac(6´)-Ib-cr, qnrS, oqxA, and qepA genes were present in 43 (89.6%), 27 (56.3%), 23 (47.9%), and one (2.1%) of the isolates, respectively. None of the isolates tested positive to qnrA, qnrB, and oqxB genes. The presence of the aac(6´)-Ib-cr gene positively correlated with resistance to ceftriaxone, cefotaxime, and gentamicin (P-values < 0.05). Conclusion: high proportions of Gram-negative bacterial isolates were resistant to quinolones and most of these isolates possessed multiple PMQR genes. There is a need to implement measures to limit the spread of these organisms.

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  • In six human studies, E. coli exhibited MDR, with prevalence ranging from 41.5% to 99.6%.

    “In six “human studies”, E. coli exhibited MDR, with the prevalence ranging from 41.5% to 99.6% [18,23,27,34,36,41].”

    From A Systematic Review on Antimicrobial Resistance in Ghana from a One Health Perspective (2024) · cites this paper as [23]

    The Results report resistance to individual antibiotics among E. coli isolates, but do not give an E. coli multidrug-resistance (MDR) prevalence. Searches for MDR, 41.5%, and 99.6% found no passage establishing that this study contributes to the claimed range across six human studies. (LLM, conf=0.73)

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Cited by

Sorted by citations

Authors
Sah, A. K., & Feglo, P. K.
Affiliations
Kwame Nkrumah University of Science and Technology (GH)
Corresponding author
Feglo, Patrick Kwame
ORCID
Feglo, Patrick Kwame
Venue
Pan African Medical Journal
Publisher
Pan African Medical Journal
Published
2022
Volume
43
Pages
207-207
ISSN
1937-8688 (ISSN-L)
DOI
10.11604/pamj.2022.43.207.33734
PMC
PMC10024564
OpenAlex
W4312661138
Cited by
4
References
2
Citations by year
2026: 2 · 2025: 1 · 2024: 1
FWCI
0.16
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Pan African Medical Journal (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Kwame Nkrumah University of Science and Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antibiotic Use and Resistance
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
Keywords
plasmid-mediated quinolone resistance, quinolone resistance genes, Gram-negative bacteria, Escherichia coli, extended-spectrum beta-lactamase, aac(6')-Ib-cr, qnrS, oqxA, qepA, ciprofloxacin resistance, nalidixic acid resistance, multidrug resistance, clinical isolates, Western Region of Ghana
MeSH terms
Anti-Bacterial Agents, Ciprofloxacin, Cross-Sectional Studies, Escherichia coli, Ghana, Humans, Microbial Sensitivity Tests, Nalidixic Acid, Plasmids, Quinolones, Drug Resistance, Bacterial

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