Cited by 286Proteoglycans and glycosaminoglycans researchCoronary Interventions and DiagnosticsAtherosclerosis and Cardiovascular Diseases

Differential Accumulation of Proteoglycans and Hyaluronan in Culprit Lesions: Insights Into Plaque Erosion

Kolodgie, Frank D., Burke, Allen P., Farb, Andrew, Weber, Deena K., Kutys, Robert, Wight, Thomas N., Virmani, Renu·2002·Arteriosclerosis, Thrombosis, and Vascular Biology

Abstract

Objective— The importance of the extracellular matrix molecules versican, biglycan, decorin, and hyaluronan in plaque instability has not been recognized. Methods and Results— Coronary lesions with acute thrombi and stable plaques were examined for the accumulation and distribution of specific proteoglycans and hyaluronan at culprit sites. The cell surface receptor for hyaluronan, CD44, and smooth muscle (SM) cell maturation markers were also assessed. Proteoglycans and hyaluronan accumulated in distinct patterns depending on plaque type. The fibrous cap of stable lesions was enriched in versican and biglycan, with considerably less staining for decorin and hyaluronan, whereas picrosirius red revealed a heavy accumulation of collagen type I. In contrast, intense staining for hyaluronan and versican was found in erosions at the plaque/thrombus interface, with weak staining for biglycan and decorin; collagen content was predominantly type III. Rupture sites showed little immunoreactivity for proteoglycans or hyaluronan. CD44 was localized along the plaque/thrombus interface in erosions, whereas in ruptures and stable plaques, it was mostly confined to inflammatory cells. Positive immunostaining for immature SM cells (SM myosin heavy chain SM1 and SMemb) was present in stable and eroded plaques, whereas the presence of SM2 and smoothelin was weak or nonexistent. Conclusions— Specific accumulation of versican, hyaluronan, and CD44 at the sites of plaque erosion implicates an involvement of these molecules in events associated with acute coronary thrombosis.

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Crossref · 10.1161/01.atv.0000034021.92658.4c

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  • In plaque erosion, VSMCs are often abundant, amidst a proteoglycan-rich ECM, enriched in type III collagen, versican and hyaluronan.

    “but VSMCs are often abundant, amidst a proteoglycan-rich ECM, enriched in type III collagen, versican and hyaluronan 193 .”

    From Vascular smooth muscle cells in atherosclerosis (2019) · cites this paper as [193]

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Authors
Kolodgie, F. D., Burke, A. P., Farb, A., Weber, D. K., Kutys, R., Wight, T. N., & Virmani, R.
Affiliations
Hope Heart Institute (US)
Venue
Arteriosclerosis, Thrombosis, and Vascular Biology
Published
2002-10
Volume
22
Issue
10
Pages
1642-1648
ISSN
  • 1079-5642 (ISSN-L)
  • 1524-4636
OpenAlex
W2119761763
Cited by
286
References
44
Citations by year
2026: 6 · 2025: 15 · 2024: 12 · 2023: 13 · 2022: 10 · 2021: 17 · 2020: 17 · 2019: 14 · 2018: 16 · 2017: 12 · 2016: 8 · 2015: 10 · 2014: 12 · 2013: 13 · 2012: 11
FWCI
3.41
Type
journal article
Language
en
Open access
closed
Locations
  • Arteriosclerosis Thrombosis and Vascular Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (R01 HL61799-02, R01 HL18645, R01HL061799)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Health Sciences › Medicine › Surgery › Coronary Interventions and Diagnostics
  • Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
Keywords
proteoglycans, hyaluronic acid, versican, biglycan, decorin, CD44, plaque erosion, plaque rupture, acute coronary thrombosis, coronary artery disease, extracellular matrix, type I collagen, type III collagen, fibrous cap
MeSH terms
Adult, Cell Differentiation, Coronary Artery Disease, Coronary Thrombosis, Extracellular Matrix, Female, Humans, Hyaluronic Acid, Immunohistochemistry, Male, Middle Aged, Muscle, Smooth, Vascular, Myocardium, Phenotype, Chondroitin Sulfate Proteoglycans, Proteoglycans, Biomarkers, Extracellular Matrix Proteins, Death, Sudden, Cardiac, Hyaluronan Receptors, Lectins, C-Type, Versicans, Decorin, Biglycan

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