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Cited by 573Nitric Oxide and Endothelin EffectsRedox biology and oxidative stressNeutrophil, Myeloperoxidase and Oxidative Mechanisms

The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.

Distinct Subcellular Localizations of Nox1 and Nox4 in Vascular Smooth Muscle Cells

Hilenski, Lula L., Clempus, Roza E., Quinn, Mark T., Lambeth, J. David, Griendling, Kathy K.·2004·Arteriosclerosis, Thrombosis, and Vascular Biology
DOI 10.1161/01.atv.0000112024.13727.2c
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Abstract

Objective— Reactive oxygen species (ROS) that act as signaling molecules in vascular smooth muscle cells (VSMC) and contribute to growth, hypertrophy, and migration in atherogenesis are produced by multi-subunit NAD(P)H oxidases. Nox1 and Nox4, two homologues to the phagocytic NAD(P)H subunit gp91 phox , both generate ROS in VSMC but differ in their response to growth factors. We hypothesize that the opposing functions of Nox1 and Nox4 are reflected in their differential subcellular locations. Methods and Results— We used immunofluorescence to visualize the NAD(P)H subunits Nox1, Nox4, and p22 phox in cultured rat and human VSMC. Optical sectioning using confocal microscopy showed that Nox1 is co-localized with caveolin in punctate patches on the surface and along the cellular margins, whereas Nox4 is co-localized with vinculin in focal adhesions. These immunocytochemical distributions are supported by membrane fractionation experiments. Interestingly, p22 phox , a membrane subunit that interacts with the Nox proteins, is found in surface labeling and in focal adhesions in patterns similar to Nox1 and Nox4, respectively. Conclusions— The differential roles of Nox1 and Nox4 in VSMC may be correlated with their differential compartmentalization in specific signaling domains in the membrane and focal adhesions.

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Crossref · 10.1161/01.atv.0000112024.13727.2c

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Research works printed but not linked: 7

  1. 10.1161/res.86.5.494 · DOI
  2. 10.1161/res.88.9.888 · DOI
  3. 10.1161/atvb.22.1.21 · DOI
  4. 2002, Circulation, 106, II
  5. 2003, FASEB J, 17, A1243.
  6. 2002, Circulation, 106, II
  7. 10.1161/hyp.32.3.459 · DOI

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Metadata

Authors
Hilenski, L. L., Clempus, R. E., Quinn, M. T., Lambeth, J. D., & Griendling, K. K.
Affiliations
Emory University (US)
ORCID
Venue
Arteriosclerosis, Thrombosis, and Vascular Biology
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2004-04
Volume
24
Issue
4
Pages
677-683
ISSN
  • 1079-5642 (ISSN-L)
  • 1524-4636
DOI
10.1161/01.atv.0000112024.13727.2c
OpenAlex
W2115239727
Cited by
573
References
39
Citations by year
2026: 2 · 2025: 3 · 2024: 5 · 2023: 15 · 2022: 9 · 2021: 13 · 2020: 14 · 2019: 21 · 2018: 22 · 2017: 25 · 2016: 17 · 2015: 23 · 2014: 36 · 2013: 39 · 2012: 53
FWCI
16.48
Type
journal article
Language
en
Open access
closed
Locations
  • Arteriosclerosis Thrombosis and Vascular Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (HL 58000, HL38206, HL58863, P01HL058000, R01HL058863)
Topics
  • Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords
NOX1, NOX4, NADPH oxidase, reactive oxygen species, vascular smooth muscle cells, subcellular localization, p22phox, caveolin, focal adhesions, vinculin, membrane compartmentalization, atherogenesis
MeSH terms
NADPH Oxidase 1, NADPH Oxidase 4, Animals, Cell Division, Cell Fractionation, Cells, Cultured, Cytoskeleton, Humans, Male, Microscopy, Fluorescence, Muscle, Smooth, Vascular, NADH, NADPH Oxidoreductases, NADPH Dehydrogenase, Oxidation-Reduction, Phosphoproteins, Vinculin, Cellular Senescence, Rats, Sprague-Dawley, Reactive Oxygen Species, Microscopy, Confocal, NADPH Oxidases, Protein Subunits, Caveolae, Focal Adhesions, Caveolins, Membrane Transport Proteins, Myocytes, Smooth Muscle, Macromolecular Substances, Caveolin 1, Rats

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