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Cited by 287

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Inhibition of Myosin Phosphatase by Upregulated Rho-Kinase Plays a Key Role for Coronary Artery Spasm in a Porcine Model With Interleukin-1β

Kandabashi, Tadashi, Shimokawa, Hiroaki, Miyata, Kenji, Kunihiro, Ikuko, Kawano, Yoji, Fukata, Yuko, Higo, Taiki, Egashira, Kensuke, Takahashi, Shosuke, Kaibuchi, Kozo, Takeshita, Akira·2000·Circulation
DOI 10.1161/01.cir.101.11.1319
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Abstract

Background —We recently demonstrated that the Rho-kinase–mediated pathway plays an important role for coronary artery spasm in our porcine model with interleukin-1β (IL-1β). In this study, we examined whether or not Rho-kinase is upregulated at the spastic site and if so, how it induces vascular smooth muscle hypercontraction. Methods and Results —Segments of the left porcine coronary artery were chronically treated from the adventitia with IL-1β–bound microbeads. Two weeks after the operation, as reported previously, intracoronary serotonin repeatedly induced coronary hypercontractions at the IL-1β–treated site both in vivo and in vitro, which were markedly inhibited by Y-27632, one of the specific inhibitors of Rho-kinase. Reverse transcription–polymerase chain reaction analysis demonstrated that the expression of Rho-kinase mRNA was significantly increased in the spastic compared with the control segment. Western blot analysis showed that during the serotonin-induced contractions, the extent of phosphorylation of the myosin-binding subunit of myosin phosphatase (MBS), one of the major substrates of Rho-kinase, was significantly greater in the spastic than in the control segment and that the increase in MBS phosphorylations was also markedly inhibited by Y-27632. There was a highly significant correlation between the extent of MBS phosphorylations and that of contractions. Conclusions —These results indicate that Rho-kinase is upregulated at the spastic site and plays a key role in inducing vascular smooth muscle hypercontraction by inhibiting myosin phosphatase through the phosphorylation of MBS in our porcine model.

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Metadata

Authors
Kandabashi, T., Shimokawa, H., Miyata, K., Kunihiro, I., Kawano, Y., Fukata, Y., Higo, T., Egashira, K., Takahashi, S., Kaibuchi, K., & Takeshita, A.
Venue
Circulation
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2000-03-21
Volume
101
Issue
11
Pages
1319-1323
ISSN
  • 0009-7322 (ISSN-L)
  • 1524-4539
DOI
10.1161/01.cir.101.11.1319
OpenAlex
W2107920372
Cited by
287
References
29
FWCI
6.53
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Inhibition of Myosin Phosphatase by Upregulated Rho-Kinase Plays a Key Role for Coronary Artery Spasm in a Porcine Model With Interleukin-1β. https://app.cochat.ai/papers/10.1161/01.cir.101.11.1319
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Kandabashi, T., Shimokawa, H., Miyata, K., Kunihiro, I., Kawano, Y., Fukata, Y., Higo, T., Egashira, K., Takahashi, S., Kaibuchi, K., & Takeshita, A. (2000). Inhibition of Myosin Phosphatase by Upregulated Rho-Kinase Plays a Key Role for Coronary Artery Spasm in a Porcine Model With Interleukin-1β. Circulation. https://doi.org/10.1161/01.cir.101.11.1319

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.