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Cited by 61Diabetes, Cardiovascular Risks, and LipoproteinsNitric Oxide and Endothelin EffectsAdvanced Glycation End Products research

Hydrolase activities in the rat aorta. I. Effects of diabetes mellitus and insulin treatment

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Wolinsky, H, Goldfischer, S, Capron, L, Capron, F, Coltoff-Schiller, B, Kasak, L·1978·Circulation Research
DOI 10.1161/01.res.42.6.821
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Abstract

Vascular disease in diabetics could arise in part from altered vessel wall catebolism. Specific activities of hydrolases in aortic smooth muscle cells from rats with streptozotocin-induced diabetes were measured. Enyzmes included: neutral alpha-glucosidase, alpha-mannosidase, and lysosomal N-acetyl beta-glucosaminidase, beta-galactosidase, cathepsin C, acid alpha-glucosidase, and acid cholesteryl esterase. After 4,8, and 11 weeks of diabetes, activities of all enzymes studied were decreased significantly in diabetic vessels, decreases ranging from 15% for cathepsin C to 62% for alpha-mannosidase. After 3 weeks of diabetes, insulin treatment for 1 week restored enzyme levels to normal. After 7 weeks of diabetes, 1 week of insulin treatment did not restore enzyme levels fully to normal (acid cholesteryl esterase was unchanged); 4 weeks of insulin did. Acid phosphatase and N-acetyl beta-glucosaminidase activities were reduced markedly in histochemical studies of diabetic aortas at all time periods and were restored by insulin treatment. Alloxan-induced diabetes gave results similar to those with streptozotocin. Significant decreases of aortic hydrolase activities, including those of lysosomes, occur in experimental diabetes mellitus and could contribute to accumulation of substrates in vascular smooth muscle cells.

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Research works printed but not linked: 4

  1. Stout RW, Bierman EL, Bmnzell JD: Atherosclerosis and disorders of lipid metabolism in diabetes. In Diabetes: Its Physiological and Biochemical Basis, edited by J. Vallance-Owen, Baltimore, t.-iversity Park Press, 1975, pp 125-170
  2. Fowler S, Wolinsky H: Lysosomes in vascular smooth muscle cells. In Vascular Smooth Muscle, Handbook of Physiology, edited by D Bohr, AP Somlyo, HV Sparks, American Physiological Society (in press)
  3. Peters TJ, Takano T, de Duve C: Subcellular fractionation studies on cells isolated from normal and atherosclerotic aorta. In Atherogenesis: Initiating Factors, Ciba Foundation Symposium 12, edited by R Porter, J Knight. New York, American Elsevier, 1973, pp 197-222
  4. Florkin M, Stolz EH (eds): Comprehensive Biochemistry, ed 2, vol 13. Amsterdam, Elsevier Publishing Co, 1965

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Authors
Wolinsky, H., Goldfischer, S., Capron, L., Capron, F., Coltoff-Schiller, B., & Kasak, L.
ORCID
Capron, L
Venue
Circulation Research
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
1978-06
Volume
42
Issue
6
Pages
821-831
ISSN
  • 0009-7330 (ISSN-L)
  • 1524-4571
DOI
10.1161/01.res.42.6.821
OpenAlex
W2063806926
Cited by
61
References
27
Citations by year
2019: 1 · 2018: 1 · 2014: 2 · 2013: 1 · 2012: 1
FWCI
7.25
Type
journal article
Language
en
Open access
Locations
  • Circulation Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Public Health Service
  • National Heart, Lung, and Blood Institute
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
Keywords
hydrolase activity, aortic smooth muscle cells, diabetes mellitus, insulin therapy, streptozotocin-induced diabetes, alloxan-induced diabetes, lysosomal enzymes, N-acetyl-beta-D-glucosaminidase, cathepsin C, alpha-mannosidase, acid phosphatase, beta-galactosidase, acid alpha-glucosidase, neutral alpha-glucosidase, substrate accumulation
MeSH terms
Alloxan, Animals, Aorta, Blood Glucose, Body Weight, Diabetes Mellitus, Experimental, Diabetic Angiopathies, DNA, Hydrolases, Insulin, Kinetics, Male, Streptozocin, Time Factors, Rats

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