The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Elevation in cytosolic free calcium concentration early in myocardial ischemia in perfused rat heart.
Abstract
Changes in cytosolic free calcium concentration during myocardial ischemia were measured by 19F NMR in 5FBAPTA-loaded perfused rat hearts. The hearts were perfused with Krebs-Henseleit buffer containing 5 microM of the acetoxymethyl ester of 5FBAPTA, which was hydrolyzed by cytosolic esterases to achieve cytosolic concentrations of 5FBAPTA of 0.12 to 0.65 mM. Cytosolic free calcium concentrations were calculated as the product of the ratio of peak areas for bound and free 5FBAPTA in the NMR spectra and the dissociation constant (708 nM). The basal cytosolic calcium concentration, measured in potassium or magnesium arrested hearts, was 252 nM, and the time-average calcium concentration in beating hearts was 630 nM. Following the onset of total ischemia, there was no immediate substantial change in cytosolic calcium despite a rapid decline in creatine phosphate and ATP and a marked increase in inorganic phosphate as monitored by 31P NMR, but by 10 minutes, there was a substantial increase in free calcium concentration. The ratio of peak areas of bound and free 5FBAPTA returned to the preischemic value during reperfusion, and there was no detectable loss of 5FBAPTA from the heart. Creatine phosphate was also restored to its preischemic level during reperfusion. These results indicate that cytosolic free calcium increases during ischemia and is not immediately associated with lethal injury. This increase in cytosolic calcium may activate degradative enzymes that eventually could compromise myocyte viability.
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Cited by
Oxygen-derived free radicals and myocardial reperfusion injury: An overview
Bolli, R. (1991) · Cardiovascular Drugs and Therapy · doi:10.1007/bf00054747 · Cited by 295
Golbach, L. A., Portelli, L. A., Savelkoul, H. F. J., Terwel, S. R., Kuster, N., de Vries, R. B. M., & Verburg-van Kemenade, B. M. L. (2016) · Environment International · doi:10.1016/j.envint.2016.01.014 · Cited by 70
Bround, M. J., Abay, E., Huo, J., Havens, J. R., York, A. J., Bers, D. M., & Molkentin, J. D. (2024) · Scientific Reports · doi:10.1038/s41598-024-57340-3 · Cited by 20
Metadata
- Authors
- Steenbergen, C., Murphy, E., Levy, L., & London, R. E.
- Venue
- Circulation Research
- Published
- 1987-05
- Volume
- 60
- Issue
- 5
- Pages
- 700-707
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- OpenAlex
- W2166977259
- Cited by
- 499
- References
- 20
- FWCI
- 23.89
- Type
- journal article
- Language
- en
- Open access
- closed
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- Steenbergen, C., Murphy, E., Levy, L., & London, R. E. (1987). Elevation in cytosolic free calcium concentration early in myocardial ischemia in perfused rat heart. Circulation Research. https://doi.org/10.1161/01.res.60.5.700
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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