The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
In vivo gene transfer and expression in normal uninjured blood vessels using replication-deficient recombinant adenovirus vectors.
Open accessAbstract
Replication-deficient recombinant adenovirus vectors do not require target cell replication for transfer and expression of exogenous genes and thus may be useful for in vivo gene therapy in the endothelium. To evaluate the feasibility of adenovirus-mediated gene transfer in vivo in normal intact blood vessels, adenovirus vectors containing the Escherichia coli lacZ gene or a human alpha 1-antitrypsin (alpha 1AT) cDNA were injected in vivo into the lumen of an occluded vessel segment of sheep jugular vein and/or carotid artery. After 15 minutes of incubation, circulation was restored; the vessels were harvested 1-28 days later and evaluated for gene transfer and expression. Three days after in vivo exposure to the lacZ adenovirus vector, the endothelium of jugular veins and carotid arteries expressed beta-galactosidase. Exposure of jugular veins and carotid arteries in vivo to the alpha 1AT adenovirus vector resulted in the expression of alpha 1AT mRNA transcripts detected by Northern analysis and in the synthesis and secretion of alpha 1AT detected by ex vivo [35S]methionine labeling. Expression with the adenovirus vectors was efficient and easily detectable 1-14 days after injection, with maximum expression at 7 days. Expression was no longer evident at 28 days. Thus, adenovirus vectors are capable of transferring exogenous genes to the endothelium of normal arteries and veins with expression for at least 2 weeks, suggesting that these vectors have the potential for a variety of cardiovascular experimental and clinical applications.
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Cites
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Adenovirus-Mediated Transfer of a Recombinant α1-Antitrypsin Gene to the Lung Epithelium in Vivo
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Self-citationWidespread long-term gene transfer to mouse skeletal muscles and heart.
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Site-Specific Gene Expression in Vivo by Direct Gene Transfer into the Arterial Wall
Nabel, E. G., Plautz, G., & Nabel, G. J. (1990) · Science · doi:10.1126/science.2119055 · Cited by 569
Palmer, T. D., Rosman, G. J., Osborne, W. R., & Miller, A. D. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.4.1330 · Cited by 479
Adenovirus–mediated in vivo gene transfer and expression in normal rat liver
Jaffe, H. A., Danel, C., Longenecker, G., Metzger, M., Setoguchi, Y., Rosenfeld, M. A., Gant, T. W., Thorgeirsson, S. S., Stratford-Perricaudet, L. D., Perricaudet, M., et al. (1992) · Nature Genetics · doi:10.1038/ng0892-372 · Cited by 449
Self-citationRecombinant Gene Expression in Vivo Within Endothelial Cells of the Arterial Wall
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MEASUREMENT OF ACTIVITY OF SINGLE MOLECULES OF β-D-GALACTOSIDASE
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Implantation of Vascular Grafts Lined with Genetically Modified Endothelial Cells
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Cited by
Mack, C. A., Patel, S. R., Schwarz, E. A., Zanzonico, P., Hahn, R. T., Ilercil, A., Devereux, R. B., Goldsmith, S. J., Christian, T. F., Sanborn, T. A., et al. (1998) · The Journal of Thoracic and Cardiovascular Surgery · doi:10.1016/s0022-5223(98)70455-6 · Cited by 305
Self-citationGenetic Modification of the Lung Directed Toward Treatment of Human Disease
Sondhi, D., Stiles, K. M., De, B. P., & Crystal, R. G. (2017) · Human Gene Therapy · doi:10.1089/hum.2016.152 · Cited by 36
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Metadata
- Authors
- Lemarchand, P., Jones, M., Yamada, I., & Crystal, R. G.
- Venue
- Circulation Research
- Published
- 1993-05
- Volume
- 72
- Issue
- 5
- Pages
- 1132-1138
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- OpenAlex
- W2144217904
- Cited by
- 257
- References
- 19
- FWCI
- 17.80
- Type
- journal article
- Language
- en
- Open access
- green
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- Lemarchand, P., Jones, M., Yamada, I., & Crystal, R. G. (1993). In vivo gene transfer and expression in normal uninjured blood vessels using replication-deficient recombinant adenovirus vectors. Circulation Research. https://doi.org/10.1161/01.res.72.5.1132
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