Myosin heavy chain isoform expression in the failing and nonfailing human heart
Abstract
Abstract —In the heart, the relative proportions of the 2 forms of the motor protein myosin heavy chain (MyHC) have been shown to be affected by a wide variety of pathological and physiological stimuli. Hearts that express the faster MyHC motor protein, α, produce more power than those expressing the slower MyHC motor protein, β, leading to the hypothesis that MyHC isoforms play a major role in the determination of cardiac contractility. We showed previously that a significant amount of αMyHC mRNA is expressed in nonfailing human ventricular myocardium and that αMyHC mRNA expression is decreased 15-fold in end-stage failing left ventricles. In the present study, we determined the MyHC protein isoform content of human heart samples of known MyHC mRNA composition. We demonstrate that αMyHC protein was easily detectable in 12 nonfailing hearts. αMyHC protein represented 7.2±3.2% of total MyHC protein (compared with ≈35% of the MyHC mRNA), suggesting that translational regulation may be operative; in contrast, there was effectively no detectable αMyHC protein in the left ventricles of 10 end-stage failing human hearts.
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Rabbit ventricles predominantly express beta-MHC (88% to >95%), similar to human ventricles (>90-95%).
“Rabbit ventricles predominately express β-MHC (88% to >95%) (Hamilton & Ianuzzo, 1991; James, et al., 2005; Suzuki, et al., 2009) similar to humans (>90–95%) (Miyata, et al., 2000; Reiser, et al., 2001).”
From Small and large animal models in cardiac contraction research: advantages and disadvantages (2014) · cites this paper as Miyata, et al., 2000
Canine ventricular myocardium predominantly expresses slow beta-MHC (>80-100%), similar to humans (>90-95%).
“The canine ventricular myocardium predominately expresses slow β-MHC (>80–100%) similar to humans (>90–95%) (Fuller, Bicer, Hamlin, Yamaguchi, & Reiser, 2007; Hamilton & Ianuzzo, 1991; Miyata, et al., 2000; Reiser, et al., 2001).”
From Small and large animal models in cardiac contraction research: advantages and disadvantages (2014) · cites this paper as Miyata, et al., 2000
Swine ventricles predominantly express beta-MHC (~100%, 87% sub-epicardium, 97% sub-endocardium), similar to humans (>90-95%).
“Swine ventricles predominately express β-MHC (~100%, 87% sub-epicardium, 97% sub-endocardium) similar to humans’ (>90–95%) (Hamilton & Ianuzzo, 1991; Locher, et al., 2011; Miyata, et al., 2000; Reiser, et al., 2001; Stelzer, et al., 2008).”
From Small and large animal models in cardiac contraction research: advantages and disadvantages (2014) · cites this paper as Miyata, et al., 2000
The predominant myosin heavy chain isoform in sheep is slow beta-MHC (~100%), similar to humans (>90-95%).
“The predominate myosin heavy chain isoform is the slow β-MHC in sheep (~100%) similar to humans (>90–95%) (Hamilton & Ianuzzo, 1991; Miyata, et al., 2000; Reiser, et al., 2001).”
From Small and large animal models in cardiac contraction research: advantages and disadvantages (2014) · cites this paper as Miyata, et al., 2000
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- Authors
- Miyata, S., Minobe, W., Bristow, M. R., & Leinwand, L. A.
- Affiliations
- From the Department of Molecular, Cellular and Developmental Biology (S.M., L.A.L.), University of Colorado (Boulder); and Division of Cardiology (W.M., M.B.), University of Colorado Health Science Center (Denver).
- University of Colorado Boulder (US)
- Venue
- Circulation Research
- Published
- 2000-03-03
- Volume
- 86
- Issue
- 4
- Pages
- 386-390
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- OpenAlex
- W2119706727
- Cited by
- 538
- References
- 38
- Citations by year
- 2026: 8 · 2025: 28 · 2024: 9 · 2023: 24 · 2022: 23 · 2021: 19 · 2020: 23 · 2019: 16 · 2018: 24 · 2017: 22 · 2016: 35 · 2015: 22 · 2014: 25 · 2013: 25 · 2012: 22
- FWCI
- 12.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Circulation Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Heart Association
- National Institutes of Health
- National Heart, Lung, and Blood Institute (HL-50560)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Function and Risk Factors
- Keywords
- myosin heavy chain, alpha-myosin heavy chain, beta-myosin heavy chain, heart failure, translational regulation, cardiac contractility, left ventricle, ventricular myocardium, mRNA-protein correlation
- MeSH terms
- Adolescent, Adult, Cardiac Output, Low, Female, Humans, Male, Middle Aged, Reference Values, RNA, Messenger, Tissue Distribution, Myosin Heavy Chains, Protein Isoforms
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