The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Elevated Levels of S -Nitrosoalbumin in Preeclampsia Plasma
Abstract
Abstract —The availability of nitric oxide (NO), which is required for the normal regulation of vascular tone, may be decreased in preeclampsia, thus contributing to the vascular pathogenesis of this pregnancy disorder. Because ascorbate is essential for the decomposition of S -nitrothiols and the release of NO, we speculated that the ascorbate deficiency typical of preeclampsia plasma might result in decreased rates of decomposition of S -nitrosothiols. We tested the hypothesis that total S -nitrosothiol and S -nitrosoalbumin concentrations are increased in preeclampsia plasma, reflecting a decreased release of NO from these major reservoirs of NO. Gestationally matched plasma samples were obtained (before labor or intravenous MgSO 4 ) from 21 women with preeclampsia and 21 women with normal pregnancy, and plasma samples were also obtained from 12 nonpregnant women of similar age and body mass index during the follicular phase of the menstrual cycle. All were nonsmokers. The assay included ultraviolet-induced decomposition of S -nitrosothiols to liberate NO captured by a florigenic reagent, 4,5-diaminofluoresceine, to produce diaminofluoresceine-Triazole. Preeclampsia plasma contained significantly higher concentrations of total S -nitrosothiols (11.1±2.9 nmol/mL) than normal pregnancy samples (9.4±1.5 nmol/mL). Even greater differences were found between preeclampsia plasma and plasma samples from normal pregnancies and nonpregnant women (294±110, 186±25, and 151±25 pmol/mg protein, respectively) when S -nitrosothiol content was expressed per milligram protein. The albumin fraction contained 49.4% of total plasma S -nitrosothiols in the control samples and 53.7% and 56.8% of plasma S -nitrosothiols in normal pregnancy and preeclampsia, respectively. The level of S -nitrosoalbumin was significantly higher in preeclampsia than in normal pregnancy or nonpregnancy plasma (6.3±1.4, 5.1±0.7, and 4.2±1.0 nmol/mL, respectively). The increased concentration of S -nitrosoalbumin in preeclampsia almost completely accounted for the increased levels of S -nitrosothiols in total plasma. Due to combined increases in nitrosothiols and decreases in protein, the preeclampsia plasma concentration of S -nitrosoalbumin was greatly increased on a per milligram of protein basis (271% and 186% compared with normal nonpregnancy and normal pregnancy plasma, respectively). We conclude that S -nitrosoalbumin and total S -nitrosothiol concentrations are significantly increased in preeclampsia plasma and may reflect insufficient release of NO groups in this condition.
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“At high ADMA concentrations, l-arginine supplementation may not overcome eNOS inhibition, indicating the need to determine l-arginine/ADMA ratios to better assess l-arginine availability (102, 106, 954).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [954]
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Detection and Imaging of Nitric Oxide with Novel Fluorescent Indicators: Diaminofluoresceins
Kojima, H., Nakatsubo, N., Kikuchi, K., Kawahara, S., Kirino, Y., Nagoshi, H., Hirata, Y., & Nagano, T. (1998) · Analytical Chemistry · doi:10.1021/ac9801723 · Cited by 1,391
Pre-eclampsia: more than pregnancy-induced hypertension
Roberts, J. M., & Redman, C. W. G. (1993) · The Lancet · doi:10.1016/0140-6736(93)90889-o · Cited by 1,239
Self-citationNitric oxide circulates in mammalian plasma primarily as an S-nitroso adduct of serum albumin.
Stamler, J. S., Jaraki, O., Osborne, J., Simon, D. I., Keaney, J., Vita, J., Singel, D., Valeri, C. R., & Loscalzo, J. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.16.7674 · Cited by 1,238
Ting, H. H., Timimi, F. K., Boles, K., Creager, S. J., Ganz, P., & Creager, M. A. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118394 · Cited by 869
Chappell, L. C., Seed, P. T., Briley, A. L., Kelly, F. J., Lee, R., Hunt, B. J., Parmar, K., Bewley, S., Shennan, A. H., Steer, P. J., et al. (1999) · The Lancet · doi:10.1016/s0140-6736(99)80010-5 · Cited by 784
Oxidative Stress in the Pathogenesis of Preeclampsia
Hubel, C. A. (1999) · Proceedings of The Society for Experimental Biology and Medicine · doi:10.1046/j.1525-1373.1999.d01-139.x · Cited by 681
Self-citationAscorbic Acid Reverses Endothelial Vasomotor Dysfunction in Patients With Coronary Artery Disease
Levine, G. N., Frei, B., Koulouris, S. N., Gerhard, M. D., Keaney, J. F., & Vita, J. A. (1996) · Circulation · doi:10.1161/01.cir.93.6.1107 · Cited by 582
Lipid peroxidation in pregnancy: New perspectives on preeclampsia
Hubel, C. A., Roberts, J. M., Taylor, R. N., Musci, T. J., Rogers, G. M., & McLaughlin, M. K. (1989) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(89)90778-3 · Cited by 445
Self-citationDemonstration of Rapid Onset Vascular Endothelial Dysfunction After Hyperhomocysteinemia
Chambers, J. C., McGregor, A., Jean-Marie, J., Obeid, O., & Kooner, J. S. (1999) · Circulation · doi:10.1161/01.cir.99.9.1156 · Cited by 427
Research works printed but not linked: 6
- 10.1161/circ.93.6.1107 · DOI
- 10.1161/circ.97.4.363 · DOI
- 10.1161/circ.99.9.1156 · DOI
- 10.1161/circ.96.5.1513 · DOI
- Tyurin VA Tyurina YY Liu SX Hubel CA Kagan VE. Fluorescence assay for quantitation of S-nitrosothiols in biological fluids and cells. Methods Enzymol. In press.
- Tyurin VA, Borisenko GG, Fabisiak JP, Hubel CA, McLaughlin MK, Roberts JM, Kagan VE. Free fatty acid (FFA-)-induced mishandling of copper (Cu) by serum albumin in preeclampsia: contribution of oxidative stress. Toxicologist. 1999;48:506. Abstract.
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Items 101–120 of 120
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Yang, Y. A., & Loscalzo, J. (2007) · Elsevier eBooks · doi:10.1016/b978-044452236-8/50010-1 · Cited by 5
Nitrite and nitrosospecies in blood and tissue: approaching the gap between bench and bedside
Rassaf, T., & Kelm, M. (2007) · Elsevier eBooks · doi:10.1016/b978-044452236-8/50013-7 · Cited by 4
Ishibashi, T., Miwa, T., Nishizawa, N., Shinkawa, I., Yoshida, J., Kawada, T., & Nishio, M. (2011) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.34.1307 · Cited by 6
Nitrogen Oxide (NO) in the Pathogenesis of Preeclampsia
Titov, V. Y., Osipov, A. N., Babenkovа, I. V., Teselkin, Y. О., Шалина, Р. И., Vykhristyuk, Y. V., & Lebedeva, S. Y. (2024) · Bulletin of Experimental Biology and Medicine · doi:10.1007/s10517-024-06255-0 · Cited by 2
Nassi, A., Kim, L. T. T., Girard, A., Griscom, L., Razan, F., Griveau, S., Thouin, L., & Fethi Bedioui, A. (2012) · Microchimica Acta · doi:10.1007/s00604-012-0860-z · Cited by 2
S-nitrosothiol and S-nitrosoalbumin levels in pre-eclampsia plasma
Moodley, J., & Gwababa, S. (2004) · Journal of Obstetrics and Gynaecology · doi:10.1080/014436104100009436 · Cited by 2
Campbell, S., Alexander-, R., Dasgupta, T. P., McGrowder, D. A., & Gordon, L. (2008) · American journal of biochemistry & biotechnology/American journal of biochemistry and biotechnology · doi:10.3844/ajbbsp.2008.265.272 · Cited by 1
Nitric Oxide Generating Polymers (NOGPs).
Hwang, S. (2007) · Deep Blue (University of Michigan)
Purification and Characterization of Novel Denitrosylases from Yeast and Mammals
Anand, P. (2012) · DukeSpace (Duke University)
Nitric oxide-mediated signaling in pulmonary endothelial cells
Stitt‐Fischer, M. S. (2008) · D-Scholarship@Pitt (University of Pittsburgh)
Boskabadi, H., Moeini, M., Tara, F., Tavallaie, S., Saber, H., Hosseini, G., Toroghi, H. M., Ferns, G. A., & Ghayour‐Mobarhan, M. (2013)
Plasmodium-Induced Nitrosative Stress in Anopheles stephensi: The Cost of Host Defense
Marie, T., & Peterson, L. (2005) · VTechWorks (Virginia Tech)
Determinants of vascular responsiveness to endogenous and exogenous activators of guanylate cyclases
Alqasim, A. A. (2006) · Doctoral thesis, University of London.
in cultured rat pulmonary vascular cells Cytoprotective effects of albumin, nitrosated or reduced,
Zhang, L., Wang, X., Chen, A. F., Liu, Z., Stoyanovsky, D. A., Pitt, B. R., Li, H., Xu, J., Wasserloos, K., Jin, L., et al. (2016)
Self-citationVascular Endothelial Cell Dysfunction in Preeclampsia
Goulopoulou, S., de Groot, C. J. M., Taylor, R. N., & Davidge, S. T. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-818417-2.00006-3
Self-citationIsmail, A. G. (2016) · theses.fr (ABES) · doi:10.70675/d94f53bbzc8bcz447bz898fz2047b4ad9fea · Cited by 1
Regulation of the redox balance and its disorders in preeclampsia
Shcherbakov, V. I., Владимировна, Ш. А., & Voevoda, M. I. (2026) · Сибирский научный медицинский журнал · doi:10.18699/ssmj20260407
Metadata
- Authors
- Tyurin, V. A., Liu, S.-X., Tyurina, Y. Y., Sussman, N. B., Hubel, C. A., Roberts, J. M., Taylor, R. N., & Kagan, V. E.
- Affiliations
- University of Pittsburgh (US)
- From the Department of Environmental and Occupational Health (V.A.T., S.-X.L., Y.Y.T., N.B.S., V.E.K.) and the Magee-Womens Research Institute and Department of Obstetrics and Gynecology (C.A.H., J.M.R., V.E.K.), University of Pittsburgh, Pittsburgh, Pa, and the Department of Obstetrics/Gynecology and Reproductive Sciences, University of California, San Francisco (R.N.T.).
- ORCID
- Venue
- Circulation Research
- Published
- 2001-06-08
- Volume
- 88
- Issue
- 11
- Pages
- 1210-1215
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- OpenAlex
- W2156597317
- Cited by
- 120
- References
- 34
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 2 · 2018: 4 · 2017: 1 · 2016: 5 · 2015: 2 · 2014: 4 · 2013: 6 · 2012: 4
- FWCI
- 16.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Circulation Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Magee-Womens Research Institute
- National Institutes of Health (5r01hl064145-04)
- National Heart, Lung, and Blood Institute (1RO1HL64145-01A1, 1RO1HL56829-01)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (P01HD030367, 5PO1HD30367-06)
- Topics
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Clinical Neurology › Neurological Complications and Syndromes
- Keywords
- preeclampsia, S-nitrosothiols, nitric oxide, ascorbate deficiency, nitric oxide release, vascular dysfunction
- MeSH terms
- Adult, Analysis of Variance, Ascorbic Acid Deficiency, Blood Proteins, Electrophoresis, Polyacrylamide Gel, Female, Fluorescent Dyes, Fluorometry, Humans, Mercaptoethanol, Nitric Oxide, Nitroso Compounds, Pre-Eclampsia, Pregnancy, Pregnancy Trimester, Second, Pregnancy Trimester, Third, Reference Values, Sensitivity and Specificity, Serum Albumin, Serum Albumin, Bovine, Body Mass Index, Oxidative Stress, S-Nitrosothiols
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