Cited by 127Neonatal and fetal brain pathologyFatty Acid Research and HealthNeuroinflammation and Neurodegeneration Mechanisms

Omega-3 Polyunsaturated Fatty Acid Supplementation Confers Long-Term Neuroprotection Against Neonatal Hypoxic–Ischemic Brain Injury Through Anti-Inflammatory Actions

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Zhang, Wenting, Hu, Xiaoming, Yang, Wei, Gao, Yanqin, Chen, Jun·2010·Stroke

Abstract

Background and Purpose— Current available therapies for neonatal hypoxia/ischemia (H/I) brain injury are rather limited. Here, we investigated the effect of omega-3 polyunsaturated fatty acids on brain damage and long-term neurological function after H/I in neonates. Methods— Female rats were treated with or without an omega-3 polyunsaturated fatty acids-enriched diet from the second day of pregnancy until 14 days after parturition. Seven-day-old neonates were subjected to H/I and euthanized 5 weeks later for evaluation of tissue loss. Neurological impairment was assessed progressively for 5 weeks after H/I by grid walking, foot fault, and Morris water maze. Activation of microglia and production of inflammatory mediators were examined up to 7 days after H/I. Results— Omega-3 polyunsaturated fatty acid supplementation significantly reduced brain damage and improved long-term neurological outcomes up to 5 weeks after neonatal H/I injury. Omega-3 polyunsaturated fatty acids exerted an anti-inflammatory effect in microglia both in an in vivo model of H/I and in in vitro microglial cultures subjected to inflammatory stimuli by inhibiting NF-κB activation and subsequent release of inflammatory mediators. Conclusions— Our results suggest that omega-3 polyunsaturated fatty acids confer potent neuroprotection against neonatal H/I brain injury through, at least partially, suppressing a microglial-mediated inflammatory response.

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  • n-3 polyunsaturated fatty acids reduce the inflammatory response, limiting the production of pro-inflammatory mediators by microglial cells following ischemic insults, and enhance glutathione-related antioxidant pathways.

    “Moreover, n-3 PUFAs reduce the inflammatory response, limiting the production of pro-inflammatory mediators by microglial cells following ischemic insults, and enhance glutathione-related antioxidant pathways [135, 136].”

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Authors
Zhang, W., Hu, X., Yang, W., Gao, Y., & Chen, J.
Affiliations
University of Pittsburgh (US)
Venue
Stroke
Published
2010-10
Volume
41
Issue
10
Pages
2341-2347
ISSN
  • 0039-2499 (ISSN-L)
  • 1524-4628
OpenAlex
W2107189849
Cited by
127
References
21
Citations by year
2026: 2 · 2025: 5 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 13 · 2020: 6 · 2019: 7 · 2018: 10 · 2017: 7 · 2016: 15 · 2015: 13 · 2014: 14 · 2013: 10 · 2012: 6
FWCI
7.18
Type
journal article
Language
en
Open access
Locations
  • Stroke (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Neurological Disorders and Stroke (NS 45048, NS-036736, R01NS056118, R01NS045048, R01NS036736, NS056118.)
Topics
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal and fetal brain pathology
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
omega-3 polyunsaturated fatty acids, neonatal hypoxic-ischemic brain injury, neuroprotection, microglia, neuroinflammation, NF-κB signaling, anti-inflammatory activity, dietary supplementation, perinatal nutrition, rat model
MeSH terms
Analysis of Variance, Animals, Animals, Newborn, Brain, Female, Maternal-Fetal Exchange, Pregnancy, Prenatal Exposure Delayed Effects, Time, Fatty Acids, Omega-3, Rats, Sprague-Dawley, Microglia, Neuroprotective Agents, Maze Learning, Reverse Transcriptase Polymerase Chain Reaction, Hypoxia-Ischemia, Brain, Rats

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