Potential of a PLA–PEO–PLA-Based Scaffold for Skin Tissue Engineering: In Vitro Evaluation
Abstract
This study aimed to investigate the in vitro behaviour of porous degradable scaffolds of the PLA-PEO-PLA-type designed prior to in vivo evaluation for skin tissue engineering. Two tri-block co-polymers were synthesized from PEO and DL-lactide and their degradation was studied under conditions that mimic a cutaneous wound environment. 3-D porous scaffolds with interconnected pores were fabricated using the salt leaching method and characterized by ESEM and Hg porosimetry. The degrading action of gamma sterilization was studied on the co-polymers. The less degraded one was selected to make porous scaffolds on which human dermal fibroblasts and human epidermal keratinocytes were cultured. The capacity of such scaffolds to act as a dermal equivalent was also considered. Colonization by human dermal fibroblasts was shown after hematoxylin staining and the production of major proteins normally found in the extracellular matrix was assessed by Western blotting of protein extracts. Finally, a skin substitute was generated by seeding human keratinocytes on the dermal equivalent and a new epidermis was characterized by using immuno-histological staining. Results show that gamma sterilization and that degradation under conditions that mimic skin wound healing were acceptable. The fact that fibroblasts produce extracellular matrix and that keratinocytes generated an epidermal barrier argues in favour of the interest of this type of porous scaffold for skin reconstruction.
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The variety of compositions offered by PLA-b-PEG-b-PLA allows a very large range of mechanical properties and degradation times.
“The variety of compositions offered by the PLAb -PEGb -PLA allows a very large range of mechanical properties and degradation times (Garric et al., 2012).”
From Mechanical behaviour׳s evolution of a PLA-b-PEG-b-PLA triblock copolymer during hydrolytic degradation (2016) · cites this paper as Garric et al., 2012
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Breche, Q., Chagnon, G., Machado, G., Girard, E., Nottelet, B., Garric, X., & Favier, D. (2016) · Journal of the Mechanical Behavior of Biomedical Materials · doi:10.1016/j.jmbbm.2016.02.015 · Cited by 30 · see citing works
“The variety of compositions offered by the PLA-b-PEG-b-PLA allows a very large range of mechanical properties and degradation times (Garric et al., 2012).”
BackgroundSelf-citation
- Authors
- Garric, X., Guillaume, O., Dabboue, H., Vert, M., & Molès, J.-P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Corresponding author
- Garric, Xavier
- Venue
- Journal of Biomaterials Science Polymer Edition
- Publisher
- Informa UK Limited
- Published
- 2012-05-08
- Volume
- 23
- Issue
- 13
- Pages
- 1687-1700
- ISSN
- 0920-5063 (ISSN-L)
- 1568-5624
- OpenAlex
- W2069079588
- Cited by
- 34
- References
- 21
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 1 · 2022: 5 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 4 · 2017: 3 · 2016: 6 · 2015: 2 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biomaterials Science Polymer Edition (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-07-EMPB-0035)
- Société Française de Dermatologie et de Pathologie Sexuellement Transmissible
- Topics
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- skin tissue engineering, porous scaffolds, poly(ethylene oxide), poly(L-lactic acid), biodegradable polymers, salt leaching, gamma irradiation sterilization, polymer degradation, dermal equivalent, human dermal fibroblasts, human epidermal keratinocytes, extracellular matrix, skin substitutes, skin barrier, wound healing, skin reconstruction
- MeSH terms
- Epidermal Cells, Cells, Cultured, Dioxanes, Epidermis, Fibroblasts, Humans, Materials Testing, Molecular Weight, Polyesters, Polyethylene Glycols, Wound Healing, Keratinocytes, Porosity, Skin, Artificial, Dermis, Tissue Engineering, Tissue Scaffolds
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