Concentration of β-ecydisone (20E) in susceptible and resistant accessions of Pfaffia glomerata infected with Meloidogyne incognita and histological characterisation of resistance
Abstract
Abstract Pfaffia glomerata is a medicinal plant widely distributed in Brazil, which is considered the world's greatest supplier of P. glomerata roots. Among active ingredients contained in this plant, the steroid β-ecydisone (20E) is the most important compound extracted from roots. This steroid presents therapeutic properties for the treatment of diabetes and haemorrhoids, besides having bioenergy, tonic and aphrodisiac effects. The root-knot nematode Meloidogyne spp. is a major limiting factor in root production. Recent studies showed resistance of accessions of P. glomerata to Meloidogyne incognita. The aims of this work were: i) to correlate the concentration of 20E with resistance and susceptibility of P. glomerata accessions to M. incognita in inoculated and non-inoculated plants; ii) to study the effect of the parasitism of M. incognita on the concentration of the steroid 20E in the roots; and iii) to clarify resistance mechanisms by comparing the response of a highly resistant UFV with a highly susceptible accession (Farm) to nematode infection. The concentration of 20E in the healthy susceptible Farm accession was significantly higher than in the healthy resistant UFV accession, showing that the resistance mechanism was not related to 20E concentrations. Plants of the Farm accession infected with M. incognita showed higher levels of 20E than the non-infected control. A positive and significant Pearson correlation coefficient was observed between 20E concentrations and gall indexes. Resistance of UFV to the root-knot nematode M. incognita was associated with unidentified factors that limited nematode penetration or emigration of second-stage juveniles and with post-penetration responses, including the hypersensitive response. Giant cells were sometimes found in the resistant cultivar, but displayed a highly vacuolated and degraded cytoplasm with thinner cell walls than those induced in the susceptible accession. Microscope observations under UV light showed a strong autofluorescence, suggesting that phenolic compounds may be involved in ginseng UFV resistance.
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Cyanotis vaga and Cyanotis arachnoidea belong to monocot species, and several species of ferns that belong to Polypodium are rich sources of ECs.
“Cyanotis vaga and Cyanotis arachnoidea belong to monocot species, and several species of ferns that belong to Polypodium are rich sources of ECs [7,8,30,31,32,33,34,35].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [34]
Taxons used in traditional medicines such as Leuzea carthamoides, Ajuga turkestanica, and several Pfaffia species have abundant ECs in their roots.
“The taxons that are used in traditional medicines such as Leuzea carthamoides, Ajuga turkestanica, and several Pfaffia species have abundant ECs in their roots.”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [34]
In Pfaffia glomerata, 20-HE displays susceptibility and resistance to Meloidogyne infection and histological alteration in roots.
“Meloidogyne spp. impair plant root development; in Pfaffia glomerata, 20-HE displays susceptibility and resistance to Meloidogyne infection and histological alteration in roots [34].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [34]
Cites
A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique
HUSSEY, R. S., & Barker, K. R. (1973) · The Plant disease reporter · Cited by 2,412 · see citing works
Phenolic Compounds and Their Role in Disease Resistance
Nicholson, R. L., & Hammerschmidt, R. (1992) · Annual Review of Phytopathology · doi:10.1146/annurev.py.30.090192.002101 · Cited by 1,400 · see citing works
An improved technique for clearing and staining plant tissues for detection of nematodes.
Bybd, D. W., Kirkpatrick, T., & Barker, K. R. (1983) · PubMed · Cited by 766 · see citing works
Resistance to and Tolerance of Plant Parasitic Nematodes in Plants
Trudgill, D. L. (1991) · Annual Review of Phytopathology · doi:10.1146/annurev.py.29.090191.001123 · Cited by 326 · see citing works
Flegg, J. J. M. (1967) · Annals of Applied Biology · doi:10.1111/j.1744-7348.1967.tb04497.x · Cited by 251 · see citing works
Functional study of the Caenorhabditis elegans secretory‐excretory system using laser microsurgery
Nelson, F. K., & Riddle, D. L. (1984) · Journal of Experimental Zoology · doi:10.1002/jez.1402310107 · Cited by 207 · see citing works
Pegard, A., Brizzard, G., Fazari, A., Soucaze, O., Abad, P., & Djian‐Caporalino, C. (2005) · Phytopathology · doi:10.1094/phyto-95-0158 · Cited by 161 · see citing works
Phytoecdysteroids: A novel defense against plant-parasitic nematodes
Soriano, I. R., Riley, I. T., Potter, M. J., & Bowers, W. S. (2004) · Journal of Chemical Ecology · doi:10.1023/b:joec.0000045584.56515.11 · Cited by 126 · see citing works
Effects of Ingested Phytoecdysteroids on the Growth and Development of Two Lepidopterous Larvae
Kubo, I., Klocke, J. A., & Asano, S. (1983) · Journal of Insect Physiology · doi:10.1016/0022-1910(83)90031-8 · Cited by 123 · see citing works
The histopathology of root-knot nematode infections in soybeans.
Dropkin, V. H., & Nelson, P. E. (1960) · Phytopathology · Cited by 115 · see citing works
Histological Characterization of Resistance to Uromyces viciae-fabae in Faba Bean
SILLERO, J. C., & Rubiales, D. (2002) · Phytopathology · doi:10.1094/phyto.2002.92.3.294 · Cited by 104 · see citing works
Insect allelochemicals from Ajuga plants
Camps, F., & Coll, J. (1993) · Phytochemistry · doi:10.1016/0031-9422(93)85139-i · Cited by 103 · see citing works
Paulson, R. E., & WEBSTER, J. M. (1972) · Physiological Plant Pathology · doi:10.1016/0048-4059(72)90005-7 · Cited by 103 · see citing works
Valette, C., Andary, C., Geiger, J. P., Sarah, J. L., & Nicole, M. (1998) · Phytopathology · doi:10.1094/phyto.1998.88.11.1141 · Cited by 103 · see citing works
Self-citationInduction Patterns of an Extensin Gene in Tobacco upon Nematode Infection
Niebel, A., Engler, J. D. A., Tire, C., Engler, G., MONTAGU, M. V., & Gheysen, G. (1993) · The Plant Cell · doi:10.1105/tpc.5.12.1697 · Cited by 101 · see citing works
Ecdysteroids in adults of the nematode, Dirofilaria immitis
Mendis, A. H. W., Rose, M. E., Rees, H. H., & Goodwin, T. W. (1983) · Molecular and Biochemical Parasitology · doi:10.1016/0166-6851(83)90098-1 · Cited by 85 · see citing works
Ecdysteroids and related molecules in animals and plants
Lafont, R. (1997) · Archives of Insect Biochemistry and Physiology · doi:10.1002/(sici)1520-6327(1997)35:1/2 · Cited by 84 · see citing works
Robbins, W. E., Kaplanis, J. N., Thompson, M. J., Shortino, T. J., & Joyner, S. C. (1970) · Steroids · doi:10.1016/s0039-128x(70)80100-3 · Cited by 81 · see citing works
Mechanisms conferring plant incompatibility to nematodes.
Kaplan, D. T., & Keen, N. T. (1980) · Cited by 79 · see citing works
Sijmons, P. C. (1993) · Plant Molecular Biology · doi:10.1007/bf00021809 · Cited by 76 · see citing works
Ascaris suum: Role of ecdysteroids in molting
Fleming, M. W. (1985) · Experimental Parasitology · doi:10.1016/0014-4894(85)90024-4 · Cited by 74 · see citing works
Anthony, F., Topart, P., Martinez, A., Silva, M. D. C., & Nicole, M. (2005) · Plant Pathology · doi:10.1111/j.1365-3059.2005.01239.x · Cited by 68 · see citing works
Self-citationPost‐infection development and histopathology of Meloidogyne arenaria race 1 on Arachis spp.
Proite, K., Carneiro, R. M. D. G., Falcão, R. E. A., Gomes, A. C. M. M., Leal‐Bertioli, S. C. M., Guimarães, P. M., & Bertioli, D. J. (2008) · Plant Pathology · doi:10.1111/j.1365-3059.2008.01861.x · Cited by 67 · see citing works
Self-citationJuvenile and moulting hormones from nematodes
Rogers, W. P. (1973) · Parasitology · doi:10.1017/s003118200004631x · Cited by 58 · see citing works
Phenol accumulation related to resistance in tomato to infection by root-knot and lesion nematodes.
Hung, C. L., & Rohde, R. A. (1973) · PubMed · Cited by 52 · see citing works
Grundler, F. M. W., Sobczak, M., & Lange, S. (1997) · Physiological and Molecular Plant Pathology · doi:10.1006/pmpp.1997.0100 · Cited by 48 · see citing works
Arnault, C., & Sl�ma, K. (1986) · Journal of Chemical Ecology · doi:10.1007/bf01041947 · Cited by 45 · see citing works
Penetration, Development and reproduction of MELOIDOGYNE ARENARIA on two new resistant VITIS SPP.
Anwar, S. A., & McKenry, M. V. (2000) · Nematropica · Cited by 42 · see citing works
Giebel, J. (1970) · Nematologica · doi:10.1163/187529270x00423 · Cited by 42 · see citing works
Invasion and Migration Behaviour of Sedentary Nematodes
von Mende, N. (1997) · Developments in plant pathology · doi:10.1007/978-94-011-5596-0_5 · Cited by 42 · see citing works
Bajaj, Y. P. S., Atwal, A. S., & Tanda, A. S. (1989) · Nematologica · doi:10.1163/002825989x00124 · Cited by 33 · see citing works
Anwar, S. A., Phillips, M., & Trudgill, D. L. (1994) · Nematologica · doi:10.1163/003525994x00436 · Cited by 19 · see citing works
Effects of ingested phytoecdysteroids in the female soft tickOrnithodoros moubata
Savolainen, V., Wüest, J., Lafont, R., & Connat, J. (1995) · Cellular and Molecular Life Sciences · doi:10.1007/bf02128751 · Cited by 16 · see citing works
Sazonalidade na produção de raízes e teor de β-ecdisona em acessos de fáfia
Júnior, C. C., Ming, L. C., & Cortez, D. A. G. (2008) · Horticultura Brasileira · doi:10.1590/s0102-05362008000300020 · Cited by 13 · see citing works
On hormonal control of moulting in Aphelenchus avenae (Nematoda: Aphelenchida)
Davies, K., & Fisher, J. (1994) · International Journal for Parasitology · doi:10.1016/0020-7519(94)90117-1 · Cited by 12 · see citing works
NATURE OF INHERITED NEMATODE RESISTANCE IN PLANTS
Bingefors, S. (1982) · Elsevier eBooks · doi:10.1016/b978-0-12-326440-4.50013-3 · Cited by 12 · see citing works
Resistência de acessos de Pfaffia glomerata a Meloidogyne incognita Raça 1
Gomes, A. M. M., Mattos, J. K. D. A., Cirotto, P. A. S., & Carneiro, R. M. D. G. (2006) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) · Cited by 6 · see citing works
Self-citation
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1 of the 38 references couldn’t be matched to a known paper.
Cited by
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Caleffi, E. R., Krausová, G., Hyršlová, I., Paredes, L. L. R., dos Santos, M. M., Sassaki, G. L., Gonçalves, R. A. C., & de Oliveira, A. J. B. (2015) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2015.06.053 · Cited by 99 · see citing works
Arif, Y., Singh, P., Bajguz, A., & Hayat, S. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23158664 · Cited by 50 · see citing works
Vardanega, R., Carvalho, P. I. N., Santos, D., & Meireles, M. (2017) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2017.05.007 · Cited by 44 · see citing works
Karyotype, genome size, and in vitro chromosome doubling of Pfaffia glomerata (Spreng.) Pedersen
Gomes, S. S. L., Saldanha, C. W., Neves, C. S., Trevizani, M., Raposo, N. R. B., Notini, M. M., Santos, M. D. O., de Campos, J. M. S., Otoni, W. C., & Viccini, L. F. (2014) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-014-0460-1 · Cited by 43 · see citing works
Techno-economic evaluation of obtaining Brazilian ginseng extracts in potential production scenarios
Vardanega, R., Carvalho, P. I. N., Albarelli, J. Q., dos Santos, D. T., & Meireles, M. A. A. (2016) · Food and Bioproducts Processing · doi:10.1016/j.fbp.2016.10.010 · Cited by 39 · see citing works
Silva, T. D., Chagas, K., Batista, D. S., Felipe, S. H. S., Louback, E., Machado, L. T., Fernandes, A. M., Buttrós, V. H., Koehler, A. D., Farias, L. M., et al. (2019) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-019-10003-9 · Cited by 18 · see citing works
Neves, C. S., Gomes, S. S. L., dos Santos, T. R., de Almeida, M. M., de Souza, Y. O., Garcia, R. M. G., Otoni, W. C., Chedier, L. M., Raposo, N. R. B., Viccini, L. F., et al. (2016) · South African Journal of Botany · doi:10.1016/j.sajb.2016.07.003 · Cited by 16 · see citing works
Ferreira, P. R. B., da Cruz, A. C. F., Batista, D. S., Nery, L. A., Andrade, I. G., Rocha, D. I., Felipe, S. H. S., Koehler, A. D., Nunes‐Nesi, A., & Otoni, W. C. (2019) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-019-01664-w · Cited by 12 · see citing works
Ecdysteroids as defensive chemicals
(2022) · Advances in Insect Physiology · doi:10.1016/bs.aiip.2022.09.001 · Cited by 11 · see citing works
Use of SFC in Extraction of Adaptogens from Brazilian Plants
Carulo, M. F. (2012) · American Journal of Analytical Chemistry · doi:10.4236/ajac.2012.312a129 · Cited by 6 · see citing works
Somavilla, L. (2011) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) · Cited by 3 · see citing works
- Authors
- Gomes, A. C. M. M., Nicole, M., Mattos, J. K., Pereira, S. I. V., Pereira, P., Silva, D. B., Vieira, R., De Capdeville, G., Moita, A. W., & Carneiro, R. M. D. G.
- Affiliations
- Embrapa (BR)
- Institut de Recherche pour le Développement (FR)
- Universidade de Brasília (BR)
- Universidade de Ribeirão Preto (BR)
- 3EMBRAPA – Recursos Genéticos e Biotecnologia, C.P. 02372, 70849-979 Brasilia, DF, Brazil
- Embrapa Hortaliças (BR)
- Venue
- Nematology
- Publisher
- Walter de Gruyter GmbH
- Published
- 2010
- Volume
- 12
- Issue
- 5
- Pages
- 701-709
- ISSN
- 1388-5545 (ISSN-L)
- 1568-5411
- OpenAlex
- W2043712385
- Cited by
- 11
- References
- 38
- Citations by year
- 2022: 2 · 2019: 2 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 1 · 2012: 1
- FWCI
- 0.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nematology (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Nematode management and characterization studies
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Keywords
- Pfaffia glomerata, Meloidogyne incognita, root-knot nematode, plant resistance mechanisms, medicinal plants, nematode susceptibility, hypersensitive response, giant cells, phenolic compounds, autofluorescence, root galls, nematode penetration
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