The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Deformation-Induced Injury of Alveolar Epithelial Cells: Effect of Frequency, Duration, and Amplitude
Abstract
The onset of ventilator-induced lung injury (VILI) is linked to a number of possible mechanisms. To isolate the possible role of alveolar epithelial deformation in the development of VILI, we have developed an in vitro system in which changes in alveolar epithelial cell viability can be measured after exposure to tightly controlled and physiologically relevant deformations. We report here a study of the relative effect of deformation frequency, duration, and amplitude on cell viability. We exposed rat primary alveolar epithelial cells to a variety of biaxial stretch protocols, and assessed deformation-induced cell injury quantitatively, using a fluorescent cell viability assay. Deformation-induced injury was found to depend on repetitive stretching, with cyclic deformations significantly more damaging than tonically held deformations. In cyclically deformed cells, injury occurred rapidly, with the majority of cell death occurring during the first 5 min of deformation. Deformation-induced injury was increased with the frequency of sustained cyclic deformations, but was not dependent on the deformation rate during a single stretch. Reducing the amplitude of cell deformations by superimposing small cyclic deformations on a tonic deformation significantly reduced cell death as compared with large-amplitude deformations with the same peak deformation.
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Cites
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Bai, H., Si, L., Jiang, A., Belgur, C., Zhai, Y., Plebani, R., Oh, C. Y., Rodas, M., Patil, A., Nurani, A., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-29562-4 · Cited by 171
Cyclic stretch-induced reorganization of the cytoskeleton and its role in enhanced gene transfer
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Metadata
- Authors
- TSCHUMPERLIN, D. J., OSWARI, J., & MARGULIES, A. S. S.
- Venue
- American Journal of Respiratory and Critical Care Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-08-01
- Volume
- 162
- Issue
- 2
- Pages
- 357-362
- ISSN
- 1073-449X (ISSN-L)
- 1535-4970
- OpenAlex
- W1888618913
- Cited by
- 347
- References
- 30
- FWCI
- 7.44
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Deformation-Induced Injury of Alveolar Epithelial Cells: Effect of Frequency, Duration, and Amplitude. https://app.cochat.ai/papers/10.1164/ajrccm.162.2.9807003
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- TSCHUMPERLIN, D. J., OSWARI, J., & MARGULIES, A. S. S. (2000). Deformation-Induced Injury of Alveolar Epithelial Cells: Effect of Frequency, Duration, and Amplitude. American Journal of Respiratory and Critical Care Medicine. https://doi.org/10.1164/ajrccm.162.2.9807003
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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