The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Quantitation of Mast Cells and Eosinophils in the Bronchial Mucosa of Symptomatic Atopic Asthmatics and Healthy Control Subjects Using Immunohistochemistry
Abstract
We have used fiberoptic bronchoscopy to obtain endobronchial biopsies in which mast cells and eosinophils were enumerated using monoclonal antibodies directed against mast cell tryptase (AA1) and the eosinophil cationic protein (EG2). Eleven symptomatic atopic asthmatics treated with beta 2-agonists alone and six normal subjects were studied. Over a period of 2 wk prior to bronchoscopy, patients recorded asthma symptom scores, bronchodilator usage, and twice-daily peak expiratory flow. Five days before bronchoscopy, methacholine responsiveness was assessed. Two biopsies were taken from the subcarinae, one of which was processed into araldite for immunostaining by the streptavidin biotin immunoperoxidase method and the other into Spurr resin for electron microscopy. The number of AA1 staining mast cells present in the bronchial mucosa was not significantly different in the epithelium or submucosa between the asthmatic and the normal subjects. However, in the biopsies from asthmatics, there were significantly greater numbers of EG2-staining eosinophils in the epithelium (median, 1.2/mm versus zero; p less than 0.005) and in the submucosa (median, 50/mm2 versus 1/mm2; p less than 0.001). Electron microscopy showed morphologic features of mast cell and eosinophil degranulation in the asthmatics. No correlation could be established between mast cell or eosinophil numbers and indices of disease activity of PC20 methacholine, which points to the complexity of mechanisms responsible for the symptoms and the airway hyperresponsiveness of asthma.
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Cites
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Standardization of bronchial inhalation challenge procedures
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Damage of the Airway Epithelium and Bronchial Reactivity in Patients with Asthma
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Self-citationSUBEPITHELIAL FIBROSIS IN THE BRONCHI OF ASTHMATICS
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Lopez, A. F., Williamson, D. J., Gamble, J. R., Begley, C. G., Harlan, J. M., Klebanoff, S. J., Waltersdorph, A., Wong, G., Clark, S. C., & Vadas, M. A. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112705 · Cited by 707
Platelet-activating factor. A potent chemotactic and chemokinetic factor for human eosinophils.
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Cited by
Asthma: From Bronchoconstriction to Airways Inflammation and Remodeling
BOUSQUET, J., JEFFERY, P. K., BUSSE, W. W., JOHNSON, M., & VIGNOLA, A. M. (2000) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.161.5.9903102 · Cited by 1,702
Induction of nitric oxide synthase in asthma
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Self-citationEosinophils: Biological Properties and Role in Health and Disease
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Dissociation between Airway Inflammation and Airway Hyperresponsiveness in Allergic Asthma
CRIMI, E., SPANEVELLO, A., NERI, M., IND, P. W., ROSSI, G. A., & BRUSASCO, V. (1998) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.157.1.9703002 · Cited by 508
Kips, J. C., O'Connor, B. J., Langley, S. J., Woodcock, A., Kerstjens, H. A. M., Postma, D. S., Danzig, M., Cuss, F., & Pauwels, R. A. (2003) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200206-525oc · Cited by 494
Metadata
- Authors
- Djukanović, R., Wilson, J. W., Britten, K. M., Wilson, S. J., Walls, A. F., Roche, W. R., Howarth, P. H., & Holgate, S. T.
- Venue
- American Review of Respiratory Disease
- Publisher
- Oxford University Press (OUP)
- Published
- 1990-10
- Volume
- 142
- Issue
- 4
- Pages
- 863-871
- ISSN
- 0003-0805 (ISSN-L)
- OpenAlex
- W2154945180
- Cited by
- 462
- References
- 25
- FWCI
- 19.93
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Quantitation of Mast Cells and Eosinophils in the Bronchial Mucosa of Symptomatic Atopic Asthmatics and Healthy Control Subjects Using Immunohistochemistry. https://app.cochat.ai/papers/10.1164/ajrccm/142.4.863
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- Djukanović, R., Wilson, J. W., Britten, K. M., Wilson, S. J., Walls, A. F., Roche, W. R., Howarth, P. H., & Holgate, S. T. (1990). Quantitation of Mast Cells and Eosinophils in the Bronchial Mucosa of Symptomatic Atopic Asthmatics and Healthy Control Subjects Using Immunohistochemistry. American Review of Respiratory Disease. https://doi.org/10.1164/ajrccm/142.4.863
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
