The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Macrophage Metalloelastase Mediates Acute Cigarette Smoke–induced Inflammation via Tumor Necrosis Factor-α Release
Abstract
The cells and proteases that mediate cigarette smoke-induced emphysema are controversial, with evidence favoring either neutrophils and neutrophil-derived serine proteases or macrophages and macrophage-derived metalloproteases as the important effectors. We recently reported that both macrophage metalloelastase (MMP-12) and neutrophils are required for acute cigarette smoke-induced connective tissue breakdown, the precursor of emphysema. Here we show how these disparate observations can be linked. Both wild-type (MMP-12 +/+) mice and mice lacking MMP-12 (MMP-12 -/-) demonstrated rapid increases in whole-lung nuclear factor-kappaB activation and gene expression of proinflammatory cytokines after cigarette smoke exposure, indicating that a lack of MMP-12 does not produce a global failure to upregulate inflammatory mediators. However, only MMP-12 +/+ mice demonstrated increased whole-lung tumor necrosis factor-alpha (TNF-alpha) protein or release of TNF-alpha from cultured alveolar macrophages exposed to smoke in vitro. Levels of whole-lung E-selectin, an endothelial activation marker, were increased in only MMP-12 +/+ mice. These findings suggest that, acutely, MMP-12 mediates smoke-induced inflammation by releasing TNF-alpha from macrophages, with subsequent endothelial activation, neutrophil influx, and proteolytic matrix breakdown caused by neutrophil-derived proteases. TNF-alpha release may be a general mechanism whereby metalloproteases drive cigarette smoke-induced inflammation.
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Cites
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
Requirement for Macrophage Elastase for Cigarette Smoke-Induced Emphysema in Mice
Hautamaki, R. D., Kobayashi, D. K., Senior, R. M., & Shapiro, S. D. (1997) · Science · doi:10.1126/science.277.5334.2002 · Cited by 1,469
Self-citationProcessing of tumour necrosis factor-α precursor by metalloproteinases
Gearing, A. J. H., Beckett, P., Christodoulou, M., Churchill, M., Clements, J., Davidson, A. H., Drummond, A. H., Galloway, W. A., Gilbert, R., Gordon, J. L., et al. (1994) · Nature · doi:10.1038/370555a0 · Cited by 1,194
Human 92- and 72-kilodalton type IV collagenases are elastases.
Senior, R. M., Griffin, G. L., Fliszar, C. J., Shapiro, S. D., Goldberg, G. I., & Welgus, H. G. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)89530-1 · Cited by 545
Self-citationElastases and emphysema. Current assessment of the protease-antiprotease hypothesis.
Janoff, A. (1985) · PubMed · doi:10.1164/arrd.1985.132.2.417 · Cited by 500
Alveolar inflammation and its relation to emphysema in smokers.
Finkelstein, R., Fraser, R. S., Ghezzo, H., & Cosio, M. G. (1995) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.152.5.7582312 · Cited by 483
Russell, R. E. K., Culpitt, S. V., DeMatos, C., Donnelly, L., Smith, M., Wiggins, J., & Barnes, P. J. (2002) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb.26.5.4685 · Cited by 448
Hunninghake, G. W., & Crystal, R. G. (1983) · PubMed · doi:10.1164/arrd.1983.128.5.833 · Cited by 447
Matrix Metalloproteinase Expression and Production by Alveolar Macrophages in Emphysema
FINLAY, G. A., O'DRISCOLL, L. R., RUSSELL, K. J., D'ARCY, E. M., MASTERSON, J. B., FITZGERALD, M. X., & O'CONNOR, C. M. (1997) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.156.1.9612018 · Cited by 440
Cigarette smoke induces interleukin-8 release from human bronchial epithelial cells.
Mio, T., Romberger, D. J., Thompson, A. B., Robbins, R. A., Heires, A., & Rennard, S. I. (1997) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.155.5.9154890 · Cited by 430
Cited by
Matrix metalloproteinases as modulators of inflammation and innate immunity
Parks, W. C., Wilson, C. L., & López-Boado, Y. S. (2004) · Nature Reviews Immunology · doi:10.1038/nri1418 · Cited by 1,929
Mediators of Chronic Obstructive Pulmonary Disease
Barnes, P. J. (2004) · Pharmacological Reviews · doi:10.1124/pr.56.4.2 · Cited by 710
Matrix Metalloproteinases as Modulators of Inflammation
MANICONE, A., & MCGUIRE, J. (2008) · Seminars in Cell & Developmental Biology · doi:10.1016/j.semcdb.2007.07.003 · Cited by 617
Matrix Metalloproteinases in Lung: Multiple, Multifarious, and Multifaceted
Greenlee, K. J., Werb, Z., & Kheradmand, F. (2007) · Physiological Reviews · doi:10.1152/physrev.00022.2006 · Cited by 474
Inflammatory cells in the airways in COPD
O'Donnell, R. (2006) · Thorax · doi:10.1136/thx.2004.024463 · Cited by 217
Metadata
- Authors
- Churg, A., Wang, R. D., Tai, H., Wang, X., Xie, C., Dai, J., Shapiro, S. D., & Wright, J. L.
- Venue
- American Journal of Respiratory and Critical Care Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-04-15
- Volume
- 167
- Issue
- 8
- Pages
- 1083-1089
- ISSN
- 1073-449X (ISSN-L)
- 1535-4970
- OpenAlex
- W2168760383
- Cited by
- 367
- References
- 39
- FWCI
- 24.52
- Type
- journal article
- Language
- en
- Open access
- closed
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- Churg, A., Wang, R. D., Tai, H., Wang, X., Xie, C., Dai, J., Shapiro, S. D., & Wright, J. L. (2003). Macrophage Metalloelastase Mediates Acute Cigarette Smoke–induced Inflammation via Tumor Necrosis Factor-α Release. American Journal of Respiratory and Critical Care Medicine. https://doi.org/10.1164/rccm.200212-1396oc
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