The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Long-Term Intratracheal Lipopolysaccharide Exposure in Mice Results in Chronic Lung Inflammation and Persistent Pathology
Abstract
Lipopolysaccharide (LPS), a major proinflammatory glycolipid component of the gram-negative bacterial cell wall, is one of the agents ubiquitously present as contaminant on airborne particles, including air pollution, organic dusts, and cigarette smoke. Chronic exposure to significant levels of LPS is reported to be associated with the development and/or progression of many types of lung diseases, including asthma, chronic bronchitis, and progressive irreversible airflow obstruction, that are all characterized by chronic inflammatory processes in the lung. In the present study, pathologic effects of long-term LPS exposure to the lung were investigated in detail. To this end, a murine model in which mice were exposed to repeated intratracheal instillation of Escherichia coli LPS was developed. We show that long-term LPS instillation in mice results in persistent chronic pulmonary inflammation, characterized by peribronchial and perivascular lymphocytic aggregates (CD4(+), CD8(+), and CD19(+)), parenchymal accumulation of macrophages and CD8(+) T cells, and altered cytokine expression. Furthermore, airway and alveolar alterations such as mucus cell metaplasia, airway wall thickening, and irreversible alveolar enlargement accompanied the chronic inflammatory response. Interestingly, the observed inflammatory and pathologic changes mimic changes observed in human subjects with chronic inflammatory lung diseases, especially chronic obstructive pulmonary disease (COPD), suggesting that this murine model could be applicable to dissect the role of inflammation in the pathogenesis of these disease conditions.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Chronic Obstructive Pulmonary Disease
(2018) · Questions for the Final FFICM Structured Oral Examination · doi:10.1017/9781108233712.025 · Cited by 2,689
Requirement for Macrophage Elastase for Cigarette Smoke-Induced Emphysema in Mice
Hautamaki, R. D., Kobayashi, D. K., Senior, R. M., & Shapiro, S. D. (1997) · Science · doi:10.1126/science.277.5334.2002 · Cited by 1,469
Chronic Obstructive Pulmonary Disease
Barnes, P. J. (2000) · New England Journal of Medicine · doi:10.1056/nejm200007273430407 · Cited by 1,078
Schindler, R., Mancilla, J., Endres, S., Ghorbani, R., Clark, S., & Dinarello, C. (1990) · Blood · doi:10.1182/blood.v75.1.40.40 · Cited by 1,012
Severity of asthma is related to endotoxin in house dust.
Michel, O., Kips, J., Duchateau, J., Vertongen, F., Robert, L., Collet, H., Pauwels, R., & Sergysels, R. (1996) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.154.6.8970348 · Cited by 576
CD8 + ve Cells in the Lungs of Smokers with Chronic Obstructive Pulmonary Disease
SAETTA, M., BARALDO, S., CORBINO, L., TURATO, G., BRACCIONI, F., REA, F., CAVALLESCO, G., TROPEANO, G., MAPP, C. E., MAESTRELLI, P., et al. (1999) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.160.2.9812020 · Cited by 489
Temann, U.-A., Geba, G. P., Rankin, J. A., & Flavell, R. A. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.7.1307 · Cited by 452
Interferon γ Induction of Pulmonary Emphysema in the Adult Murine Lung
Wang, Z., Zheng, T., Zhu, Z., Homer, R. J., Riese, R. J., Chapman, H. A., Shapiro, S. D., & Elias, J. A. (2000) · The Journal of Experimental Medicine · doi:10.1084/jem.192.11.1587 · Cited by 415
Bacterial Endotoxin Is an Active Component of Cigarette Smoke
Hasday, J. D., Bascom, R., Costa, J. J., Fitzgerald, T., & Dubin, W. (1999) · Chest · doi:10.1378/chest.115.3.829 · Cited by 388
Effect of inhaled endotoxin on bronchial reactivity in asthmatic and normal subjects
Michel, O., Duchateau, J., & Sergysels, R. (1989) · Journal of Applied Physiology · doi:10.1152/jappl.1989.66.3.1059 · Cited by 257
Cited by
Animal models of chronic obstructive pulmonary disease
Wright, J. L., Cosio, M., & Churg, A. (2008) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.90200.2008 · Cited by 447
Airway Bacterial Load and FEV 1 Decline in Patients with Chronic Obstructive Pulmonary Disease
Wilkinson, T. M. A., Patel, I. S., Wilks, M., Donaldson, G. C., & Wedzicha, J. A. (2003) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200210-1179oc · Cited by 370
Respiratory Syncytial Virus Up-regulates TLR4 and Sensitizes Airway Epithelial Cells to Endotoxin
Monick, M. M., Yarovinsky, T. O., Powers, L. S., Butler, N. S., Carter, A. B., Gudmundsson, G., & Hunninghake, G. W. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m308093200 · Cited by 276
Ectopic lymphoid tissues and local immunity
Carragher, D. M., Rangel-Moreno, J., & Randall, T. D. (2008) · Seminars in Immunology · doi:10.1016/j.smim.2007.12.004 · Cited by 274
In vitro and ex vivo anti-inflammatory activity of quercetin in healthy volunteers
Boots, A. W., Wilms, L. C., Swennen, E. L. R., Kleinjans, J. C. S., Bast, A., & Haenen, G. R. M. M. (2008) · Nutrition · doi:10.1016/j.nut.2008.03.023 · Cited by 270
Metadata
- Authors
- Vernooy, J. H. J., Dentener, M. A., van Suylen, R. J., Buurman, W. A., & Wouters, E. F. M.
- Venue
- American Journal of Respiratory Cell and Molecular Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-01-01
- Volume
- 26
- Issue
- 1
- Pages
- 152-159
- ISSN
- 1044-1549 (ISSN-L)
- 1535-4989
- OpenAlex
- W1974528705
- Cited by
- 253
- References
- 29
- FWCI
- 8.08
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Long-Term Intratracheal Lipopolysaccharide Exposure in Mice Results in Chronic Lung Inflammation and Persistent Pathology. https://app.cochat.ai/papers/10.1165/ajrcmb.26.1.4652
- The paper
- Vernooy, J. H. J., Dentener, M. A., van Suylen, R. J., Buurman, W. A., & Wouters, E. F. M. (2002). Long-Term Intratracheal Lipopolysaccharide Exposure in Mice Results in Chronic Lung Inflammation and Persistent Pathology. American Journal of Respiratory Cell and Molecular Biology. https://doi.org/10.1165/ajrcmb.26.1.4652
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
