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5-Flourouracil Loaded N,O-Carboxymethyl Chitosan Nanoparticles as an Anticancer Nanomedicine for Breast Cancer
Abstract
Chitosan and its carboxymethyl derivatives are smart biopolymers that are non-toxic, biocompatible, biodegradable and hence found applications in biomedical field. In the current work, we have developed 5-fluorouracil (5-FU) loaded N,Ocarboxymethyl chitosan (N,O-CMC) nanoparticles (mean diameter: 80 +/- 20 nm, zeta potential: +52.47 +/- 2 mV) for cancer drug delivery. Drug entrapment efficiency (65%) and in vitro drug release studies were carried out spectrophotometricaly. Cellular internalization of the drug loaded nanoparticles was confirmed by fluorescent microscopy and flow cytometric analysis. Results of anticancer activity via MTT, apoptosis and caspase 3 assays showed the toxicity of the drug loaded nanoparticles towards breast cancer cells. As a whole these results indicates the potential of 5-FU loaded N,O-CMC nanoparticles in breast cancer chemotherapy in which the side effects of conventional chemo treatment could be reduced. Furthermore, the results of in vitro hemolytic assay and coagulation assay substantiate the blood compatibility of the system as well.
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The Ge/Pec hydrogels can be utilized as a safe carrier for controlled drug delivery.
“and can be utilized as a safe carrier for controlled drug delivery.52-56”
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Biomedical Applications of Chitosan and Its Derivative Nanoparticles
Zhao, D., Yu, S., Sun, B., Gao, S., Guo, S., & Zhao, K. (2018) · Polymers · doi:10.3390/polym10040462 · Cited by 607
“They are used in drugs and gene delivery [55,56], in biosensors [57], and in fractionated images [58,59].”
BackgroundBiocompatibility of Chitosan Carriers with Application in Drug Delivery
Rodrigues, S., Dionísio, M., Remuñán‐López, C., & Grenha, A. (2012) · Journal of Functional Biomaterials · doi:10.3390/jfb3030615 · Cited by 393
“Chitosan carriers have been frequently tested by means of the above mentioned assays, with a predominance of the MTT assay, not only using the original polymer [30,82–84], but also chitosan derivatives [85,86].”
MethodAnticancer Activity of Chitosan, Chitosan Derivatives, and Their Mechanism of Action
Adhikari, H. S., & Yadav, P. N. (2018) · International Journal of Biomaterials · doi:10.1155/2018/2952085 · Cited by 364
Upadhyaya, L., Singh, J., Agarwal, V. K., & Tewari, R. P. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.04.043 · Cited by 276
Polyphenol-chitosan conjugates: Synthesis, characterization, and applications
Hu, Q., & Luo, Y. (2016) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2016.05.109 · Cited by 276
Fonseca‐Santos, B., & Chorilli, M. (2017) · Materials Science and Engineering C · doi:10.1016/j.msec.2017.03.198 · Cited by 270
Chitosan-based nanoparticles as drug delivery systems: a review on two decades of research
Naskar, S., Kuotsu, K., & Sharma, S. (2018) · Journal of drug targeting · doi:10.1080/1061186x.2018.1512112 · Cited by 217
“CSNPs have been largely used for the delivery of anti-cancer drugs including methotrexate [112], 5-flourouracil [113,114], cysplatin [115], epirubicin [116], curcumin [117,118], mitomycin C [119], docetaxel [120], doxorubicin (DOX) [49,121], paclitaxel [122] and tamoxifen [123], focussing to augment anti-tumour effica…”
BackgroundDing, Y., Shen, S., Sun, H., Sun, K., Liu, F., Qi, Y., & Yan, J. (2014) · Materials Science and Engineering C · doi:10.1016/j.msec.2014.12.036 · Cited by 215
Anitha, A., Deepa, N., Chennazhi, K. P., et al. (2014) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2014.06.004 · Cited by 174
Self-citationSun, X., Liu, C., Omer, A. M., Lu, W., Zhang, S., Jiang, X., Wu, H., Yu, D., & Ouyang, X. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.01.140 · Cited by 174
Anitha, A., Sreeranganathan, M., Chennazhi, K. P., Lakshmanan, V., & Jayakumar, R. (2014) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2014.04.017 · Cited by 156
Self-citationA Review on Drug Delivery System for Tumor Therapy
Liu, G., Yang, L., Chen, G., Xu, F., Yang, F., Yu, H., Li, L., Dong, X., Han, J., Cao, C., et al. (2021) · Frontiers in Pharmacology · doi:10.3389/fphar.2021.735446 · Cited by 147
Polysaccharides based nanomaterials for targeted anti-cancer drug delivery
Dheer, D., Arora, D., Jaglan, S., Rawal, R. K., & Shankar, R. (2016) · Journal of drug targeting · doi:10.3109/1061186x.2016.1172589 · Cited by 132
“is actively working on curcumin loaded N,O-carboxymethyl chitosan nanoparticles (N,OCMC) [40] and 5-fluorouracil (5-FU) loaded N,O-CMC nanoparticles for anticancer drug delivery [44].”
BackgroundDevelopment and evaluation of 5-fluorouracil loaded chitin nanogels for treatment of skin cancer
Sabitha, M., Rejinold, N. S., Nair, A., Lakshmanan, V., Nair, S. V., & Jayakumar, R. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2012.07.060 · Cited by 128
Self-citationBiodegradable polymers for targeted delivery of anti-cancer drugs
Doppalapudi, S., Jain, A., Domb, A. J., & Khan, W. (2016) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2016.1156671 · Cited by 125
“5-Fluorouracilloaded carboxymethyl chitosan nanoparticles showed preferential toxicity in breast cancer cells [57].”
BackgroundMaya, S., Kumar, L. G., Sarmento, B. F. C. C., Rejinold, N. S., Menon, D., Nair, S. V., & Jayakumar, R. (2013) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2012.12.032 · Cited by 113
Self-citationGold nanoparticles enhance 5-fluorouracil anticancer efficacy against colorectal cancer cells
Safwat, M. A., Soliman, G. M., Sayed, D., & Attia, M. A. (2016) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2016.09.076 · Cited by 109
Mariadoss, A. V. A., Vinayagam, R., Senthilkumar, V., Paulpandi, M., Murugan, K., Xu, B., Muthukaliannan, G. K., Kotakadi, V. S., & David, E. K. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.03.031 · Cited by 106
Nivethaa, E. A. K., Dhanavel, S., Narayanan, V., et al. (2014) · RSC Advances · doi:10.1039/c4ra11615a · Cited by 105
Carboxymethyl chitosan represses tumor angiogenesis in vitro and in vivo
Jiang, Z., Han, B., Li, H., Yang, Y. G., & Liu, W. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2015.04.040 · Cited by 101
Wang, J., Zhao, W., Zhang, Z., Liu, X., Xie, T., Wang, L., Xue, Y., & Zhang, Y. (2024) · Advanced Materials · doi:10.1002/adma.202308915 · Cited by 104
Versatile carboxymethyl chitin and chitosan nanomaterials: a review
Narayanan, D., Jayakumar, R., & Chennazhi, K. P. (2014) · Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.1301 · Cited by 95
Self-citationSmitha, K. T., Anitha, A., Furuike, T., et al. (2012) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2012.11.031 · Cited by 80
Self-citationLiu, K., Chen, W., Yang, T., et al. (2017) · International Journal of Nanomedicine · doi:10.2147/ijn.s147028 · Cited by 80
“In vitro hemolysis assay of PMs In vitro hemolysis caused by PMs was determined by threewavelength colorimetry.(46) Fresh blood was taken from different”
Emerging potential of 5-Fluorouracil-loaded chitosan nanoparticles in cancer therapy
Dongsar, T. T., Dongsar, T. S., Gupta, N., Almalki, W. H., Sahebkar, A., & Kesharwani, P. (2023) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2023.104371 · Cited by 72
Guo, A., Wang, F., Lin, W., Xu, X., Tang, T., Shen, Y., & Guo, S. (2014) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2014.03.024 · Cited by 67
Beidokhti, H. R. N., Ghaffarzadegan, R., Mirzakhanlouei, S., Ghazizadeh, L., & Dorkoosh, F. A. (2016) · AAPS PharmSciTech · doi:10.1208/s12249-015-0445-3 · Cited by 59
Naeem, F., Khan, S., Jalil, A., Ranjha, N. M., Riaz, A., Haider, M. S., Sarwar, S., Saher, F., & Afzal, S. (2017) · BioImpacts · doi:10.15171/bi.2017.21 · Cited by 58
Anti-cancer activity of naringenin loaded smart polymeric nanoparticles in breast cancer
Yıldırım, M., Acet, Ö., Yetkin, D., et al. (2022) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2022.103552 · Cited by 57
Nivethaa, E. A. K., Saminathan, D., Rebekah, A., et al. (2016) · Materials Science and Engineering C · doi:10.1016/j.msec.2016.04.080 · Cited by 57
Chitosan cross-linked docetaxel loaded EGF receptor targeted nanoparticles for lung cancer cells
Maya, S., Sarmento, B. F. C. C., Lakshmanan, V., Menon, D., Seabra, V., & Jayakumar, R. (2014) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2014.06.009 · Cited by 53
Self-citationWang, R., Zhi, X., Yao, S., & Zhang, Y. (2015) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2015.05.030 · Cited by 52
Balahura, L.-R., Dinescu, S., Balaș, M., Cernencu, A. I., Lungu, A., Vlăsceanu, G. M., Iovu, H., & Costache, M. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13081189 · Cited by 47
“However, in a study performed by Bertheau et al., it was suggested that TP53 mutation status may differ dependent on the chemotherapeutic treatment administered [41].”
Narayanan, D., Anitha, A., Jayakumar, R., et al. (2013) · Molecular Pharmaceutics · doi:10.1021/mp400184v · Cited by 44
Self-citationLai, J. (2013) · Drug Design Development and Therapy · doi:10.2147/dddt.s53759 · Cited by 38
“2 The use of delivery carriers made of natural 3–5 or synthetic 6–8 materials is able to increase the action of drug, thereby achieving a greater pharmacological response.”
Li, Y., Kim, J. H., Choi, E. H., & Han, I. (2019) · Scientific Reports · doi:10.1038/s41598-019-40371-6 · Cited by 38
Sahatsapan, N., Rojanarata, T., Ngawhirunpat, T., et al. (2021) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2021.102481 · Cited by 38
Kim, J. H., Kim, S., Kwon, I., et al. (2012) · Materials Letters · doi:10.1016/j.matlet.2012.12.078 · Cited by 33
“It has been identified as a biomaterial for wound dressing [8] and drug delivery [9].”
BackgroundSalahuddin, N. A., Awad, S., & Elfiky, M. N. (2022) · RSC Advances · doi:10.1039/d2ra02717h · Cited by 32
Obeidat, W. M., & Lahlouh, I. K. (2025) · AAPS PharmSciTech · doi:10.1208/s12249-025-03100-z · Cited by 34
“…and nanoparticles have been either encapsulated or attached to chitosan nanoparticles to date such as Metho-trexate [421], 5-Flourouracil [422, 423], Epirubicin [424], Curcumin [425, 426], Mitomycin C [427], Docetaxel [428], Doxorubicin [429, 430], Paclitaxel [431], Ascorbate [432], Cisplatin…”
Nivethaa, E. A. K., Baskar, S., Martin, C. A., et al. (2020) · Scientific Reports · doi:10.1038/s41598-020-60888-5 · Cited by 32
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Nivethaa, E. A. K., Saminathan, D., Narayanan, V., et al. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.10.066 · Cited by 32
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Li, D., Tohti, M., Fu, Y., et al. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.130453 · Cited by 30
Chinnaiyan, S. K., Mary, S. A., Kannan, P. R., Gopinath, A., & Madhan, B. (2019) · IET Nanobiotechnology · doi:10.1049/iet-nbt.2019.0007 · Cited by 31
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Sivanesan, I., Muthu, M., Gopal, J., Hasan, N., Ali, S. K., Shin, J., & Oh, J. (2021) · Nanomaterials · doi:10.3390/nano11030821 · Cited by 25
Enhance antimicrobial activity of ZnO nanomaterial׳s (QDs and NPs) and their analytical applications
Wahab, R., Khan, F., Lutfullah, et al. (2014) · Physica E Low-dimensional Systems and Nanostructures · doi:10.1016/j.physe.2014.04.017 · Cited by 23
Wang, F., Zhang, R., Wu, Q., et al. (2014) · ACS Applied Materials & Interfaces · doi:10.1021/am5064052 · Cited by 23
Wang, F., Deng, Z., Yang, Z., et al. (2021) · The Journal of Physical Chemistry C · doi:10.1021/acs.jpcc.1c01801 · Cited by 22
Pourmadadi, M., Aslani, A., Hassan, D., Sani, A., Rahdar, A., Medina, D. I., Abdouss, M., & Ferreira, L. F. R. (2023) · OpenNano · doi:10.1016/j.onano.2023.100177 · Cited by 21
Han, I., Rana, J. N., Kim, J., Choi, E. H., & Kim, Y. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14020465 · Cited by 20
“The method used to prepare a chitosan scaffold has been described in previous reports [26,27].”
MethodEl-Houssiny, A. S., Kamel, N. A., Soliman, A. A. F., et al. (2022) · Advances in Natural Sciences Nanoscience and Nanotechnology · doi:10.1088/2043-6262/ac6c22 · Cited by 15
Jiang, Y., Cao, Y., Yao, Y., Zhang, D., & Wang, Y. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.140144 · Cited by 16
Biomedical applications carboxymethyl chitosans
Biswas, S., Ahmed, T., Islam, M. M., et al. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-817966-6.00014-5 · Cited by 13
Ehsan, I., Kumari, L., Sen, R., et al. (2022) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2022.103689 · Cited by 13
Carboxymethyl chitosan in advanced drug-delivery applications
Das, S. S., Kar, S., Singh, S. K., et al. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-819336-5.00006-6 · Cited by 12
Nivethaa, E. A. K., Dhanavel, S., Narayanan, V., et al. (2021) · RSC Advances · doi:10.1039/d0ra08537e · Cited by 11
Chitosan-based drug delivery systems in cancer therapeutics
Padhi, S., Behera, A., Hasnain, M. S., & Nayak, A. K. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-819336-5.00011-x · Cited by 12
Nanogels: A New Dawn in Antimicrobial Chemotherapy
Sahu, P. K., Das, D., Kashaw, V., Iyer, A. K., & Kashaw, S. K. (2017) · Elsevier eBooks · doi:10.1016/b978-0-323-52733-0.00005-7 · Cited by 13
Cao, Z., Wang, X., Cheng, X., Wang, J., & Tang, R. (2017) · International Journal of Polymeric Materials · doi:10.1080/00914037.2016.1252346 · Cited by 8
Narayanan, D., Gopikrishna, J., Nair, S. V., & Menon, D. (2013) · Advances in polymer science · doi:10.1007/12_2012_199 · Cited by 8
Self-citationResearch Progress of Chitosan-based Multifunctional Nanoparticles in Cancer Targeted Therapy
Lai, C., Lin, S., Liu, W., & Jin, Y. (2023) · Current Medicinal Chemistry · doi:10.2174/0929867330666230416153352 · Cited by 7
Salahuddin, N. A., & Awad, S. (2021) · Chemical Papers · doi:10.1007/s11696-021-01625-8 · Cited by 7
“The FTIR spectrum of 5-FU has shown abroad band between 3500 and 3000 cm−1 (–NH stretching vibrations) (Anitha 2012), absorption band in the region 3100–3000 cm−1 (C–H stretching).”
BackgroundJayaraj, K., Gopi, S., Rajeswari, A., Christy, E. J. S., & Pius, A. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-817968-0.00004-4 · Cited by 7
Malik, Z., Parveen, R., Zahiruddin, S., et al. (2023) · Combinatorial Chemistry & High Throughput Screening · doi:10.2174/1386207326666230609104038 · Cited by 5
Chitosan-Based Nanoformulation as Carriers of Small Molecules for Tissue Regeneration
Narayan, S. (2019) · Springer eBooks · doi:10.1007/978-981-15-0263-7_11 · Cited by 4
Liu, W., Yan, Y., Dong, Y., Cao, J., Bao-xin, L., & Cao, L. (2024) · Food Bioscience · doi:10.1016/j.fbio.2024.105599 · Cited by 3
Rathore, S., Rajoriya, V., Kushwah, V., et al. (2021) · Current Nanomedicine · doi:10.2174/2468187311666210301112644 · Cited by 3
Bassani, H. P., Valenga, F., Sierakowski, M. R., et al. (2013) · International Journal of Phytomedicine · Cited by 3
Synthesis, Characterization and Biomedical Applications of Chitosan and Its Derivatives
Junginer, H., & Sadeghi, A. M. M. (2013) · CRC Press eBooks · doi:10.1201/b15636-4 · Cited by 3
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Basic concepts and advances in hydrogel and nanogel formulations
Sahu, P. K., Kashaw, S. K., Kashaw, V., Begum, J. P. S., & Dahiya, R. (2024) · Elsevier eBooks · doi:10.1016/b978-0-12-819979-4.00001-5 · Cited by 2
Chitosan Nanoparticles as Carrier for Anticancer Drugs: An Overview
Salar, R. K., & Kumar, N. (2018) · Springer eBooks · doi:10.1007/978-981-10-4702-2_8 · Cited by 2
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Sahu, P. K., Kashaw, S. K., Sau, S., & Iyer, A. K. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-818433-2.00012-1 · Cited by 2
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Vasile, C., Nistor, M. T., & Cojocariu, A.-M. (2014) · CRC Press eBooks · doi:10.1201/b17271-4 · Cited by 4
Nanomedicines in Drug Delivery from Synthetic and Natural Sources to Their Clinical Applications
Singha, L. R., Das, P., Ahmed, N., Das, M. K., Das, S., & Deka, T. (2020) · Springer eBooks · doi:10.1007/978-981-15-6255-6_7 · Cited by 1
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Sahu, P. K., Kashaw, S. K., Kashaw, V., & Iyer, A. K. (2023) · Springer eBooks · doi:10.1007/978-981-99-0538-6_11 · Cited by 1
Sathasivam, T., Gugler, M. C., & Pushpamalar, J. (2021) · Nanotechnology in the life sciences · doi:10.1007/978-3-030-61021-0_10 · Cited by 1
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comprehensive overview of drug delivery systems for tumor treatment
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Metadata
- Authors
- Anitha, A., Chennazhi, K. P., Nair, S. V., & Jayakumar, R.
- Affiliations
- Amrita Institute of Medical Sciences and Research Centre (IN)
- Venue
- Journal of Biomedical Nanotechnology
- Publisher
- American Scientific Publishers
- Published
- 2012-02-01
- Volume
- 8
- Issue
- 1
- Pages
- 29-42
- ISSN
- 1550-7033 (ISSN-L)
- OpenAlex
- W2077306877
- Cited by
- 79
- References
- 0
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 6 · 2022: 5 · 2021: 12 · 2020: 4 · 2019: 6 · 2018: 4 · 2017: 5 · 2016: 7 · 2015: 3 · 2014: 11 · 2013: 6 · 2012: 4
- FWCI
- 3.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biomedical Nanotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Physical Sciences › Chemistry › Organic Chemistry › Heterocyclic Compound Synthesis and Bioactivity
- Keywords
- 5-fluorouracil, N,O-carboxymethyl chitosan, chitosan nanoparticles, breast cancer, drug delivery, cancer nanomedicine, encapsulation efficiency, in vitro drug release, apoptosis, caspase-3, hemocompatibility, coagulation tests, biodegradable biopolymers
- MeSH terms
- Animals, Antimetabolites, Antineoplastic, Blood Coagulation Tests, Breast Neoplasms, Cell Survival, Drug Carriers, Female, Flow Cytometry, Fluorouracil, Hemolysis, Humans, Particle Size, Apoptosis, Cell Line, Tumor, Chitosan, Mice, Caspase 3, Nanoparticles
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