Retinol dehydrogenases RDH11 and RDH12 in the mouse retina: Expression levels during development and regulation by oxidative stress
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PURPOSE: RDH11 and RDH12 are closely related retinol dehydrogenases expressed in the retina. RDH12 has been linked to the early-onset retinal dystrophy Leber congenital amaurosis, whereas RDH11 has not been associated with human disease. To understand their physiological roles, the authors investigated their expression during development and their regulation by light-induced oxidative stress in mouse retina. METHODS: Quantitative RT-PCR and immunoblot analysis were used for quantification of RDH11 and RDH12 during development and oxidative stress. Expression during development was measured between embryonic day (E) 12 and postnatal day (P) 210 (7 months) in C57BL/6 mouse eyes. Expression during light-induced oxidative stress was measured between 2 and 24 hours of exposure to light in BALB/c mouse retina. RESULTS: The RDH11 level was low and remarkably constant during development and oxidative stress. RDH12 expression started at P7 and increased until P30 to approximately sevenfold higher than RDH11. Oxidative stress induced by exposure to constant bright light led to a rapid and significant decrease of RDH12 protein. CONCLUSIONS: The low and constant expression of RDH11 suggested a housekeeping function for this enzyme. The onset of RDH12 expression during the maturation of photoreceptor cells suggested a function related to the visual process. The light-induced rapid decrease of RDH12 protein, preceding the decrease of the mRNA, suggested a specific degradation of the protein rather than a regulation of gene expression.
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The close proximity (<350 kb) of two adjacent genes that regulate homologous recombination (ZFYVE26 and RAD51B), two visual genes (RDH11 and RDH12), and PLEKHH1 represents a case of adaptive linkage.
“We hypothesize that the close proximity (<350 kb) of two adjacent genes that regulate homologous recombination (ZFYVE26 and RAD51B, which directly interact with BRCA2), two visual genes (RDH11 and RDH12) related to retinol metabolism and dark adaptation (62), and one of our candidate domestication genes, PLEKHH1 (S2.4 in Dataset S2), represents such a case of adaptive linkage.”
From Comparative analysis of the domestic cat genome reveals genetic signatures underlying feline biology and domestication (2014) · cites this paper as [62]
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- Authors
- Kanan, Y., Wicker, L. D., Al-Ubaidi, M. R., Mandal, N. A., & Kasus-Jacobi, A.
- Affiliations
- University of Oklahoma Health Sciences Center (US)
- From the Departments of Cell Biology and
- Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma.
- Venue
- Investigative Opthalmology & Visual Science
- Published
- 2008-03-01
- Volume
- 49
- Issue
- 3
- Pages
- 1071
- ISSN
- 0146-0404 (ISSN-L)
- 1552-5783
- PMC
- PMC2366097
- OpenAlex
- W2017576225
- Cited by
- 30
- References
- 35
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 2 · 2022: 1 · 2020: 2 · 2019: 4 · 2018: 1 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 4
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Investigative Ophthalmology & Visual Science (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Eye Institute (P30EY012190, R01EY14052, R01EY014052)
- National Center for Research Resources (P20RR017703)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Keywords
- retinal development, oxidative stress, photoreceptor maturation, Leber congenital amaurosis, protein degradation, gene expression regulation, vitamin A metabolism, mouse retina
- MeSH terms
- Aging, Alcohol Oxidoreductases, Animals, Light, Mice, Inbred BALB C, Mice, Inbred C57BL, Oxidoreductases, Radiation Injuries, Experimental, Retina, Retinal Degeneration, RNA, Messenger, Transcription, Genetic, Immunoblotting, In Situ Hybridization, Mice, Knockout, Oxidative Stress, Gene Expression Regulation, Developmental, Reverse Transcriptase Polymerase Chain Reaction, Retinal Dehydrogenase, Mice
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