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Cited by 240Erythrocyte Function and PathophysiologyLipid Membrane Structure and BehaviorHemoglobinopathies and Related Disorders

The DOI matches its Crossref record; 30 of the 32 references it cites were found in a registry or an index.

IS HEMOGLOBIN AN ESSENTIAL STRUCTURAL COMPONENT OF HUMAN ERYTHROCYTE MEMBRANES?*

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Weed, Robert I., Reed, Claude F., Berg, George·1963·Journal of Clinical Investigation
DOI 10.1172/jci104747PMC PMC289318
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Abstract

This investigation of human red cell membranes was undertaken in order to determine whether the presence of hemoglobin in the membrane is es- sential for maintenance of the membrane's struc- tural integrity, which has been evaluated by stud- ies of the shape, lipid content and composition, protein content, osmotic responsiveness, and certain enzymic activities of erythrocyte ghosts from which virtually all of the cellular hemoglobin had been removed.Many studies have been done with red cell "ghosts," or posthemolytic residues, because such preparations offer the possibility of analyzing certain physiological, biochemical, electron micro- scopic, and immunologic properties attributable to the membranes themselves, apart from the intracel- lular contents.Representative techniques for preparation of red cell ghosts include hemolysis in distilled water, the effects of which were stud- ied first by Bernstein and his associates (1), the freeze-thaw method described by Jorpes (2), the use of chemical lysins such as digitonin and sa- ponin (3) and the use of 0.1%o sodium chloride saturated with CO2 (4), both as described by Ponder, and the method of successive osmotic hemolysis proposed by Hillier and Hoffman (5).Recently, Danon, Nevo, and Marikovsky (6) have described a modification of the technique of Hil- lier and Hoffman which they have termed "gradual osmotic lysis."This method provides a high yield of ghosts that bear a remarkable resemblance

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  • Low haemoglobin or low haematocrit can lead to a diminished supply of oxygen to the tissues and therefore have a direct effect on endurance performance.

    “Low Hgb, or low HCT, the volume of red blood cells compared with total blood volume, can lead to a diminished supply of oxygen to the tissues, and therefore have a direct effect on endurance performance.”

    From How does hormone transition in transgender women change body composition, muscle strength and haemoglobin? Systematic review with a focus on the implications for sport participation (2021) · cites this paper as [20]

    The Methods describe hematocrit values used to prepare red-cell samples, and the Results examine hemoglobin removal from erythrocyte membranes (page numbers unavailable). Neither those passages nor searches for oxygen transport, tissue oxygen supply, anemia, or exercise address the claimed effect on endurance performance. (LLM, conf=0.77)

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  1. Ponder, E. On properties of the red cell ghost. I. Volume and shape changes during haemolysis and "reversible of haemolysis." J. exp. Biol. 1942, 18, 257.
  2. Danon, D., A. Nevo, and Y. Marikovsky. Prepara- tion of erythrocyte ghosts by gradual haemolysis in hypotonic aqueous solution. Bull. Res. Coun. Israel E 1956, 6, 36.
  3. Carson, P. E., S. L. Schrier, and A. S. Alving. In- activation of glucose-6-phosphate dehydrogenase in human erythrocytes (abstract). J. Lab. clin. Med. 1956, 48, 794.
  4. Kirkman, H. N. Characterization of partially puri- fied glucose-6-phosphate dehydrogenase from nor- mal and primaquine-sensitive erythrocytes (ab- stract). Fed. Proc. 1959, 18, 261.
  5. Erickson, B. N., H. W. Williams, S. S. Bernstein, I. Avrin, R. L. Jones, and I. G. Macy. Lipid dis- tribution of posthemolytic residue or stroma of erythrocytes. J. biol. Chem. 1938, 122, 515.
  6. Ponder, E. Red cell structure and its breakdown. Protoplasmatologia: Handbuch der protoplasma- forschung. Vienna, Springer, 1955, 10, 53.
  7. Westerman, M. P., L. E. Pierce, and W. N. Jensen. Red cell and plasma lipid studies in sickle cell anemia (abstract). Blood 1960, 15, 422.
  8. Hoffman, J. F. Physiological characterization of hu- man red blood cell ghosts. J. gen. Physiol. 1958, 42, 9.

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Metadata

Authors
Weed, R. I., Reed, C. F., & Berg, G.
Affiliations
University of Rochester Medicine (US)
Venue
Journal of Clinical Investigation
Publisher
American Society for Clinical Investigation
Published
1963-04-01
Volume
42
Issue
4
Pages
581-588
ISSN
0021-9738 (ISSN-L)
DOI
10.1172/jci104747
PMC
PMC289318
OpenAlex
W2161381317
Cited by
240
References
32
Citations by year
2026: 1 · 2025: 5 · 2024: 7 · 2023: 9 · 2022: 6 · 2021: 10 · 2020: 15 · 2019: 4 · 2018: 10 · 2017: 8 · 2016: 7 · 2015: 12 · 2014: 4 · 2013: 11 · 2012: 2
FWCI
9.79
Type
journal article
Language
en
Open access
Locations
  • Journal of Clinical Investigation (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
University of Rochester
Topics
  • Health Sciences › Medicine › Physiology (medical) › Erythrocyte Function and Pathophysiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Health Sciences › Medicine › Genetics (clinical) › Hemoglobinopathies and Related Disorders
Keywords
hemoglobin, erythrocyte membrane, membrane integrity, erythrocyte ghosts, osmotic lysis, membrane lipid composition, erythrocyte shape
MeSH terms
Erythrocyte Membrane, Erythrocytes, Hemoglobins, Humans

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