A New Radioimmunoassay for Plasma l-Triiodothyronine: Measurements in Thyroid Disease and in Patients Maintained on Hormonal Replacement
Open accessAbstract
A new procedure for the radioimmunoassay of l-triiodothyronine (T(3)) in human plasma is described in which the iodothyronines are separated from the plasma proteins before incubation with a specific antiserum to T(3). The antibody bound and free T(3) are separated with dextran-coated charcoal. In this system, the mean recovery of T(3) added to plasma was 97.9% and both in vitro conversion of l-thyroxine (T(4)) to T(3) and cross-reaction between T(4) and the anti-T(3) antibody were undetectable (less than 0.1%). The assay procedure allowed the measurement of T(3) in up to 0.5 ml of plasma resulting in improved assay sensitivity (6 ng/100 ml). The mean plasma T(3) in normal subjects was 146+/-24 ng/100 ml (sd). Mean T(3) concentration was increased in hyperthyroidism (665+/-289 ng/100 ml) and decreased in hypothyroidism (44+/-26 ng/100 ml). In patients with severe hypothyroidism, plasma T(3) was between 7 and 30 ng/100 ml. Plasma T(3) concentration was relatively constant throughout the day in three euthyroid subjects. In contrast, in hypothyroid subjects on replacement therapy with T(3), a T(4): T(3) combination or desiccated thyroid plasma T(3) was markedly elevated for several hours after ingestion of the medication. Plasma T(3) was unchanged throughout the day in patients treated with T(4). Thus, insofar as plasma T(3) levels are concerned, replacement therapy with T(4) appears to mimic the euthyroid state more closely than other preparations.
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In DTE patients, serum T3 returns to within normal range 12-24 hours after the last dose.
“serum T3 returns to within normal range 12–24 hours after the last dose.”
From Liothyronine and Desiccated Thyroid Extract in the Treatment of Hypothyroidism (2020) · cites this paper as [49]
The Results describe changes in T3 after desiccated thyroid, but do not state that it returns to the normal range within 12–24 hours. The reported return to initial euthyroid values at 24 hours concerns patients taking T3 alone, not desiccated thyroid (Results, Fig. 5 versus Fig. 8). (LLM, conf=0.84)
Experimental — accuracy not yet measuredPatients receiving DTE exhibit an elevation in serum T3 that peaks at about 3 hours after taking the tablet.
“patients receiving DTE exhibit an elevation in serum T3 that peaks at about 3 hours after taking the tablet”
From Liothyronine and Desiccated Thyroid Extract in the Treatment of Hypothyroidism (2020) · cites this paper as [49]
The Results (page not provided) report that plasma T3 rose significantly after desiccated thyroid in two of three patients, but remained unchanged in the third. The passage does not establish that T3 peaked about three hours after the tablet was taken. (LLM, conf=0.87)
Experimental — accuracy not yet measuredThe T3 spike can be seen after either LT3 or DTE administration.
“it can be seen after either LT3 or DTE administration (48,49).”
From Liothyronine and Desiccated Thyroid Extract in the Treatment of Hypothyroidism (2020) · cites this paper as [49]
In DTE patients, serum T3 might fluctuate above the upper limit of the reference range depending on the dose taken.
“this might fluctuate above the upper limit of the reference range depending on the dose taken”
From Liothyronine and Desiccated Thyroid Extract in the Treatment of Hypothyroidism (2020) · cites this paper as [49]
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- Authors
- Surks, M. I., Schadlow, A. R., & Oppenheimer, J. H.
- Affiliations
- Albert Einstein College of Medicine (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1972-12-01
- Volume
- 51
- Issue
- 12
- Pages
- 3104-3113
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC332992
- OpenAlex
- W1994027777
- Cited by
- 188
- References
- 2
- Citations by year
- 2024: 6 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 5 · 2019: 6 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 2 · 2012: 1
- FWCI
- 12.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Cancer Diagnosis and Treatment
- Keywords
- triiodothyronine, radioimmunoassay, thyroid dysfunction, hyperthyroidism, hypothyroidism, thyroid hormone replacement, levothyroxine, desiccated thyroid, diurnal variation
- MeSH terms
- Antibodies, Anti-Idiotypic, Antigen-Antibody Reactions, Blood Proteins, Charcoal, Cross Reactions, Dextrans, Humans, Hyperthyroidism, Hypothyroidism, Iodine Isotopes, Radioimmunoassay, Thyroid Diseases, Thyroid Hormones, Thyroxine, Triiodothyronine
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