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Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human Plasma
Open accessAbstract
A radioimmunoassay has been developed that permits reliable measurements of plasma arginine vasopressin (AVP) at concentrations as low as 0.5 pg/ml in sample volumes of 1 ml or less. Nonhormonal immunoreactivity associated with the plasma proteins is eliminated by acetone precipitation before assay, leaving unaltered a component that is immunologically and chromatographically indistinguishable from standard AVP. Storage of plasma results in a decline in AVP concentration and, thus, must be carefully regulated. The plasma AVP values obtained by our method approximate the anticipated levels and vary in accordance with physiologic expections. In recumbent normal subjects, plasma AVP ranged from (mean +/-SD) 5.4+/-3.4 pg/ml after fluid deprivation to 1.4+/-0.8 pg/ml after water loading, and correlated significantly with both plasma osmolality (r=0.52; P<0.001) and urine osmolality (r=0.77; P<0.001). After fluid restriction, plasma AVP was uniformly normal relative to plasma osmolality in patients with nephrogenic diabetes insipidus and primary polydipsia but was distinctly subnormal in all patients with pituitary diabetes insipidus. The infusion of physiologic amounts of posterior pituitary extract caused a dose-related rise in plasma vasopressin that afterwards declined at the expected rate (t(1/2)=22.5+/-4 min). We conclude that, when used appropriately, our radioimmunoassay method provides a useful way of assessing AVP function in man.
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“Robertson et al. showed that two radioimmunoassays for vasopressin yielded measurements in unextracted plasma that were at least two orders of magnitude greater than those inferred from other evidence, and which did not fluctuate in parallel with endogenous authentic vasopressin (33,58).”
From Intranasal Oxytocin: Myths and Delusions (2016) · cites this paper as [58]
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Cites
Desbuquois, B., & Aurbach, G. D. (1971) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-33-5-732 · Cited by 1,295
Ahmed, A. B., George, B. C., Gonzalez-Auvert, C., & Dingman, J. F. (1967) · Journal of Clinical Investigation · doi:10.1172/jci105504 · Cited by 170
Physiologic Studies of Antidiuretic Hormone by Its Direct Measurement in Human Plasma *
Czaczkes, J. W., Kleeman, C. R., Koenig, M., & Boston, R. (1964) · Journal of Clinical Investigation · doi:10.1172/jci105038 · Cited by 157
The regulation of antidiuretic hormone release in man
Segar, W. E., & Moore, W. W. (1968) · Journal of Clinical Investigation · doi:10.1172/jci105900 · Cited by 155
A Radioimmunoassay for Parathyroid Hormone in Man
Reiss, E., & Canterbury, J. M. (1968) · Experimental Biology and Medicine · doi:10.3181/00379727-128-33050 · Cited by 139
Metabolism of antidiuretic hormones
Lauson, H. D. (1967) · The American Journal of Medicine · doi:10.1016/0002-9343(67)90091-5 · Cited by 133
The effect of a high water intake on the kidney's ability to concentrate the urine in man
de Wardener, H. E., & Herxheimer, A. (1957) · The Journal of Physiology · doi:10.1113/jphysiol.1957.sp005873 · Cited by 132
Radioimmunoassay of Arginine Vasopressin in Human Plasma
Beardwell, C. G. (1971) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-33-2-254 · Cited by 116
Immunoassay of plasma vasopressin in man
Robertson, G. L., Klein, L. A., Roth, J., & Gorden, P. (1970) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.66.4.1298 · Cited by 83
Purification of Oxytocin and Vasopressin by Way of a Protein Complex
Acher, R., Light, A., & du Vigneaud, V. (1958) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)68038-5 · Cited by 74
Roth, J., Glick, S. M., Klein, L. A., & Petersen, M. J. (1966) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-26-6-671 · Cited by 74
Evolution of antidiuretic hormones and their functions
Sawyer, W. H. (1967) · The American Journal of Medicine · doi:10.1016/0002-9343(67)90088-5 · Cited by 58
Yoshida, S., Motohashi, K., Ibayashi, H., & Okinaka, S. (1963) · PubMed · Cited by 55
Binding Properties of Bovine Neurophysins I and II: An Equilibrium Dialysis Study
Breslow, E., & Walter, R. (1972) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)12724-7 · Cited by 54
Physiologic investigation of posterior pituitary binding proteins neurophysin I and neurophysin II
ROBINSON, A. G., Zimmerman, E. A., & FRANTZ, A. G. (1971) · Metabolism · doi:10.1016/0026-0495(71)90040-0 · Cited by 52
Tentative Identification of Arginine Vasotocin in Human Cerebrospinal Fluid
Pavel, S. I. (1970) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-31-4-369 · Cited by 51
Immunochemical Studies with Lysine Vasopressin
Permutt, M. A., Parker, C. W., & Utiger, R. D. (1966) · Endocrinology · doi:10.1210/endo-78-4-809 · Cited by 44
Miller, M., & Moses, A. M. (1969) · Endocrinology · doi:10.1210/endo-84-3-557 · Cited by 44
Radioimmunoassay of Vasopressin: Application to Unextracted Human Urine
Oyama, S. N., Kagan, A., & Glick, S. M. (1971) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-33-5-739 · Cited by 40
Wu, W.-H., & Rockey, J. H. (1969) · Biochemistry · doi:10.1021/bi00835a005 · Cited by 40
Hyperuricemia, a Concomitant of Congenital Vasopressin-Resistant Diabetes Insipidus in the Adult
Görden, P., Robertson, G. L., & Seegmiller, J. E. (1971) · New England Journal of Medicine · doi:10.1056/nejm197105132841903 · Cited by 30
RADIOIMMUNOASSAY FOR PLASMA ANTIDIURETIC HORMONE
Johnston, C. I. (1972) · Journal of Endocrinology · doi:10.1677/joe.0.0520069 · Cited by 29
Antidiuretic hormone levels in normal subjects.
Ww, M. (1971) · PubMed · Cited by 20
Effect of +Gz and +Gx acceleration on peripheral venous ADH levels in humans.
Rogge, J. D., Moore, W. W., Segar, W. E., & Fasola, A. F. (1967) · Journal of Applied Physiology · doi:10.1152/jappl.1967.23.6.870 · Cited by 16
The development of a radioimmunoassay and a plasma extraction method for vasopressin.
Cr, E., Chard, T., KITAU, M. J., & Forsling, M. L. (1970) · PubMed · Cited by 14
[Radioimmunological system for the determination of the antidiuretic hormone].
Ménard, J., & Milliez, P. (1970) · PubMed · Cited by 1
Flagged first, then by citations
Research works printed but not linked: 3
- Lauson, H. B. 1960. Vasopressin and oxytocin in the plasma of man and other mammals. In Hormones In Human Plasma. H. N. Antoniades, editor. Little, Brown and Company, Boston. 1st edition. 225.
- Verney, E. B. 1947. The antidiuretic hormone and fac- tors which determine its release. Proc. R. Soc. Lond. B. Biol. Sci. 135: 25.
- Share, L. 1969. Extracellular fluid volume and vaso- pressin secretion. In Frontiers in Neuroendocrinology. WV. F. Ganong and L. Martini, editors. The Oxford University Press, London. 1st edition. 183.
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Sorted by citations
Assay for the Measurement of Copeptin, a Stable Peptide Derived from the Precursor of Vasopressin
Morgenthaler, N. G., Struck, J., Alonso, C., & Bergmann, A. (2005) · Clinical Chemistry · doi:10.1373/clinchem.2005.060038 · Cited by 1,166
The Role of Blood Osmolality and Volume in Regulating Vasopressin Secretion in the Rat
Dunn, F. L., Brennan, T. J., Nelson, A. E., & Robertson, G. L. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107521 · Cited by 687
Intranasal Oxytocin: Myths and Delusions
Leng, G., & Ludwig, M. (2016) · Biological Psychiatry · doi:10.1016/j.biopsych.2015.05.003 · Cited by 616
The osmoregulation of vasopressin
Robertson, G. L., Shelton, R. L., & Athar, S. (1976) · Kidney International · doi:10.1038/ki.1976.76 · Cited by 524
Osmotic and nonosmotic control of vasopressin release
Schrier, R. W., Berl, T., & Anderson, R. J. (1979) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.1979.236.4.f321 · Cited by 500
Metadata
- Authors
- Robertson, G. L., Mahr, E. A., Athar, S., & Sinha, T.
- Affiliations
- Larue Carter Hospital (US)
- ORCID
- Robertson, Gary L.
- Venue
- Journal of Clinical Investigation
- Published
- 1973-09-01
- Volume
- 52
- Issue
- 9
- Pages
- 2340-2352
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC333039
- OpenAlex
- W1984984454
- Cited by
- 787
- References
- 26
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 15 · 2023: 11 · 2022: 17 · 2021: 11 · 2020: 13 · 2019: 10 · 2018: 13 · 2017: 14 · 2016: 25 · 2015: 14 · 2014: 18 · 2013: 11 · 2012: 15
- FWCI
- 23.58
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Nephrology › Electrolyte and hormonal disorders
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroendocrine regulation and behavior
- Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
- Keywords
- vasopressin, radioimmunoassay, acetone precipitation, plasma osmolality, urine osmolality, fluid deprivation, water loading, nephrogenic diabetes insipidus, primary polydipsia, posterior pituitary extract
- MeSH terms
- Acetone, Antibodies, Antigen-Antibody Reactions, Arginine, Blood Preservation, Blood Pressure, Chromatography, Gel, Chromatography, Ion Exchange, Cross Reactions, Diabetes Insipidus, Diuresis, Humans, Iodine Isotopes, Lysine, Methods, Osmolar Concentration, Oxytocin, Pituitary Hormones, Posterior, Radioimmunoassay, Trimethoprim, Urine, Vasopressins, Vasotocin
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