The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Rat and human colonic mucins bind to and inhibit adherence lectin of Entamoeba histolytica.
Open accessAbstract
Establishment of adherence by Entamoeba histolytica is mediated by a 170-kD Gal/GalNAc inhibitable lectin and is required for cytolysis and phagocytosis of mammalian target cells. We studied the biochemical mechanisms of the in vitro interaction between rat and human colonic mucins and axenic E. histolytica trophozoites. Crude mucus prevented amebic adherence to Chinese hamster ovary (CHO) cells by up to 70%. Purification of the colonic mucins by Sepharose 4B chromatography, nuclease digestion, and cesium chloride gradient centrifugation resulted in a 1,000-fold enrichment of the inhibitory mucins. Purified rat mucin inhibited amebic adherence to and cytolysis of homologous rat colonic epithelial cells. Oxidation and enzymatic cleavage of rat mucin Gal and GalNAc residues completely abrogated mucin inhibition of amebic adherence. The binding of rat 125I-mucin to amebae was galactose specific, saturable, reversible, and pH dependent. A monoclonal antibody specific for the 170-kD amebic Gal/GalNAc lectin completely inhibited the binding of rat 125I-mucin. Rat mucin bound to Affigel affinity purified the amebic lectin from conditioned medium. Colonic mucin glycoproteins act as an important host defense by binding to the parasite's adherence lectin, thus preventing amebic attachment to and cytolysis of host epithelial cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269
Cited by
Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier
Martens, E. C., Neumann, M., & Desai, M. S. (2018) · Nature Reviews Microbiology · doi:10.1038/s41579-018-0036-x · Cited by 711
Petri, W. A., Haque, R., & Mann, B. J. (2002) · Annual Review of Microbiology · doi:10.1146/annurev.micro.56.012302.160959 · Cited by 326
Self-citationImmune responses against protozoan parasites: a focus on the emerging role of Nod-like receptors
Gurung, P., & Kanneganti, T.-D. (2016) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-016-2212-3 · Cited by 57
Martínez-Ocaña, J., Maravilla, P., & Olivo-Díaz, A. (2020) · Revista do Instituto de Medicina Tropical de São Paulo · doi:10.1590/s1678-9946202062064 · Cited by 16
Metadata
- Authors
- Chadee, K., Petri, W. A., Innes, D. J., & Ravdin, J. I.
- Venue
- Journal of Clinical Investigation
- Published
- 1987-11-01
- Volume
- 80
- Issue
- 5
- Pages
- 1245-1254
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W1995980810
- Cited by
- 286
- References
- 1
- FWCI
- 8.32
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Rat and human colonic mucins bind to and inhibit adherence lectin of Entamoeba histolytica. https://app.cochat.ai/papers/10.1172/jci113199
- The paper
- Chadee, K., Petri, W. A., Innes, D. J., & Ravdin, J. I. (1987). Rat and human colonic mucins bind to and inhibit adherence lectin of Entamoeba histolytica. Journal of Clinical Investigation. https://doi.org/10.1172/jci113199
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
