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Cited by 104Circadian rhythm and melatoninStress Responses and CortisolImmunotoxicology and immune responses

Seasonal modulation of the circadian time structure of circulating T and natural killer lymphocyte subsets from healthy subjects.

Open access
Lévi, F A, Canon, C, Touitou, Y, Reinberg, A, Mathé, G·1988·Journal of Clinical Investigation
DOI 10.1172/jci113333PMC PMC329583
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Abstract

A seasonal modulation of the circadian time structure of circulating T and natural killer (NK) lymphocyte subtypes was documented in five healthy men aged 24-36 yr. Venous blood was obtained every 4 h for 24 h from each subject in January, March, June, August, and November 1984. Three subjects were also studied in April and/or August and/or November 1983 for the T subsets only. Mononuclear cells were isolated on Ficoll-Paque gradient and aliquots were incubated with OKT3, OKT4, OKT8, or HNK-1 monoclonal antibodies for characterizing all, T, T helper, T suppressor-cytotoxic, and NK lymphocytes, respectively, under an epifluorescence microscope. An effect of both sampling time and study month was statistically validated (P less than 0.01) with both two-way analysis of variance and cosinor for the peripheral counts in total, pan-T, T helper, and NK lymphocytes (cells per cubic millimeter). Seasonal changes affected both the circadian patterns and the 24-h mean values. Thus the double amplitude (total extent of variation) of the circadian rhythm in circulating total, T and T helper lymphocytes varied between 0 in March (P greater than 0.30; no rhythm) and up to 46-68% of the 24-h-mean (M) in November, with acrophases (times of maximum, 0) localized in the first half of the night (P less than 0.001). Maximal values were found at 8:30 h for both T suppressor-cytotoxic and NK lymphocytes; a smaller second peak was also found at 20:30 h, and a 12-h rhythm was validated by cosinor (P less than 0.0001), with no patient change in waveform along the year scale. A circannual rhythm was statistically validated by cosinor for total (0 in November), pan-T (0 in March), T suppressor-cytotoxic (0 in December), and NK lymphocytes (0 in October). A rhythm with a period equal to 6 mo was found for circulating T helper cells with 0 occurring both in April and October. Seasonal variations in the incidence of several immunologically related diseases may correspond to an endogenous circannual time structure.

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  • However, given low-to-normal total CD4+ and CD8+ T-cells, the apparent decline in memory T-cells could also be explained by the low point of normal T-cell circannual kinetics at the 2-year follow-up.

    “However, in light of the low-to-normal total CD4+ and CD8+ T-cells, this apparent decline could also be explained by the low point of normal T-cell circannual kinetics at the 2-year follow-up [44].”

    From Immune reconstitution and clinical recovery following anti-CD28 antibody (TGN1412)-induced cytokine storm (2021) · cites this paper as [44]

    The Circannual rhythms section reports seasonal variation in OKT8+ T-cell counts and a six-month rhythm in OKT4+ counts, supporting the possibility of normal fluctuations in these populations. The paper studied healthy men, however, and did not measure memory T cells or establish that the cited two-year follow-up fell at a seasonal low point. (LLM, conf=0.85)

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  • Over 2 years, T-cell subsets displayed circannual kinetics, especially in the CD25+/CD28+ subsets incorporating Tregs.

    “Over 2 years, T-cell subsets displayed circannual kinetics [44, 45], especially noted in the CD25+/CD28+ subsets, incorporating Tregs.”

    From Immune reconstitution and clinical recovery following anti-CD28 antibody (TGN1412)-induced cytokine storm (2021) · cites this paper as [44]

    The Circannual rhythms section reports annual rhythms in OKT3+ and OKT8+ T-cell subsets; the Discussion notes that some individuals were studied in the preceding year. However, the Lymphocyte surface marker determinations section lists OKT3, OKT4, OKT8, and HNK-1—not CD25 or CD28—and the paper does not identify a Treg subset or find rhythms especially in one. Page numbers were not available in our copy. (LLM, conf=0.90)

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Cites

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2 of the 47 references couldn’t be matched to a known paper.

Research works printed but not linked: 2

  1. [12]12. Schwab, R. G. 1971. Circannian testicular periodicity in the European starling in the absence of photoperiodic change. In Biochronometry. Proceedings of a Symposium at the National Acad- emy of Sciences USA.
  2. [17]17. evi, F., C. Canon, Y. Touitou, J. Sulon, M. Mechkouri, E. Demey-Ponsart, J. P. Touboul, J. M. Vannetzel, I. Mowzowicz, A. Reinberg, and G. Mathe. 1988. Circadian and circahemidian rhythms in circulating T lymphocyte subtypes and plasma concentrations of testosterone, total and free cortisol in five healthy men. Clin. Exp. Immunoe. In press.

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Authors
Lévi, F. A., Canon, C., Touitou, Y., Reinberg, A., & Mathé, G.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Pitié-Salpêtrière Hospital (FR)
ORCID
Venue
Journal of Clinical Investigation
Publisher
American Society for Clinical Investigation
Published
1988-02-01
Volume
81
Issue
2
Pages
407-413
ISSN
0021-9738 (ISSN-L)
DOI
10.1172/jci113333
PMC
PMC329583
OpenAlex
W1964130172
Cited by
104
References
47
Citations by year
2026: 1 · 2025: 1 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 4 · 2018: 1 · 2015: 1 · 2014: 4 · 2013: 4
FWCI
3.51
Type
journal article
Language
en
Open access
Locations
  • Journal of Clinical Investigation (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
Keywords
circadian rhythm, seasonal variation, T cells, natural killer cells, T helper cells, suppressor/cytotoxic T cells, lymphocyte subsets, circannual rhythm, peripheral blood lymphocyte count, healthy men
MeSH terms
Adult, Antibodies, Monoclonal, Circadian Rhythm, Humans, Killer Cells, Natural, Leukocyte Count, Male, Seasons, T-Lymphocytes

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